Comparative analysis of antioxidant and antiproliferative activities of Rhodomyrtus tomentosa extracts prepared with various solvents

Abstract

Rhodomyrtus tomentosa (Aiton) Hassk. has a wide spectrum of pharmacological effects and has been used to treat wounds, colic diarrhoea, heartburns, abscesses and gynaecopathy. The potential antiproliferative activities of R. tomentosa extracts from different solvents were evaluated in vitro on HepG2, MCF-7 and HT 29 cell lines while antioxidant activity was monitored by radical scavenging assay (DPPH), copper reducing antioxidant capacity (CUPRAC) and β-carotene bleaching assay. Extracts from R. tomentosa show the viability of the cells in concentration-dependent manner. According to the IC50 obtained, the ethyl acetate extracts showed significant antiproliferative activity on HepG2 (IC50 11.47 ± 0.280 μg/mL), MCF-7 (IC50 2.68 ± 0.529 μg/mL) and HT 29 (IC50 16.18 ± 0.538 μg/mL) after 72 h of treatment. Bioassay guided fractionation of the ethyl acetate extract led to the isolation of lupeol. Methanol extracts show significant antioxidant activities in DPPH (EC50 110.25 ± 0.005 μg/ml), CUPRAC (EC50 53.84 ± 0.004) and β-carotene bleaching (EC50 58.62 ± 0.001) due to the presence of high total flavonoid and total phenolic content which were 110.822 ± 0.017 mg butylated hydroxytoluene (BHT)/g and 190.467 ± 0.009 mg gallic acid (GAE)/g respectively. Taken together, the results extracts show the R. tomentosa as a potential source of antioxidant and antiproliferative efficacy.

A Comprehensive Assessment of Bioactive Metabolites, Antioxidant and Antiproliferative Activities of Cyclocarya paliurus (Batal.) Iljinskaja Leaves

“…As hydrogen donor, reactive oxygen quenchers, reducing agent and free radical scavenging agent in redox reactions, phenolic compounds are very important for antioxidant activity [38]. Our results showed that phenolics had a greater contribution to antioxidant activity than triterpenoid in the extracts of C. paliurus leaves, which confirmed the above viewpoint.…”

“…Compared with other plant species, the scavenging capacity of DPPH radical and reducing power in the ethanol extracts of C. paliurus leaves were weaker than the ethanol extracts of pyrola leaves, but the scavenging capacity of ABTS was similar between the two plants from specific locations [34]. Most populations were higher than goji berry and Aspalathus Linearis and some populations were comparable to R. tomentosa in terms of the scavenging capacity of DPPH radical [36][37][38]. The scavenging capacity of ABTS of some populations was higher than specific goji berry and Solanaceae species [36,39].…”

Combined impacts of zein coating enriched with methanolic and ethanolic extracts of sour orange peel and vacuum packing on the shelf life of refrigerated rainbow trout

“…In 2017, Hamid et al . reported that the inhibition of DPPH free radicals in the extracts depends on the concentration, and by increasing the concentration of the extract the inhibitory effect was increased . In contrast, in 2015, Erbil & Digrak observed that the free radical‐scavenging capacity of DPPH decreased with an increase in the concentrations of methanolic extract of lilac sage and balansa…”

Chemical composition, antibacterial activity and action mechanism of different extracts from hawthorn (Crataegus pinnatifida Bge.)

“…The solubility of flavonoids and polyphenols can be affected by aromatic ring, glycosyl form and side chain binding. The more hydroxyl groups in the molecule, the stronger of the polarity rises, and easy to dissolve in high polar solvents 17 . The results from the study was similar to previous study that the methanol was most effective in extracting flavonoids and polyphenols from plants 18 .…”