Comparative analysis of an endopolygalacturonase coding gene in isolates of seven Fusarium species

Posada, M.L.; Patiño, Belén; Heras, Aitor De La; Mirete, Salvador; Vázquez, Carmen M.; González-Jaén, M. Teresa

doi:10.1017/s0953756200003154

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…The ccen-1 gene has at least two homologues in the genome of C. cubensis and has a high sequence similarity to that of C. parasitica (enpg-1) (Gao et al, 1996). High sequence similarity of endoPGs in related fungi is not surprising and has been reported previously in fungi (Posada et al, 2000;Chimwamurombe et al, 2001).…”

Section: Discussionsupporting

confidence: 78%

Molecular Analysis of an Endopolygalacturonase Gene from a Eucalyptus Canker Pathogen, Cryphonectria cubensis

et al. 2002

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Cryphonectria cubensis causes a serious Eucalyptus canker disease. Fungal cell wall degrading enzymes (CWDEs) are important during the early stages of interaction of the fungus with Eucalyptus. To improve our understanding of the molecular regulation of the interaction of Eucalyptus and C. cubensis, the relevant genes involved in this interaction should be identified, cloned and studied. The aim of this study was, therefore, to clone the endopolygalacturonase (endoPG) gene of C. cubensis. C. cubensis was grown on a medium supplemented with Eucalyptus cell wall extracts. Degenerate primers were designed to amplify part of the endoPG gene from C. cubensis genomic DNA. The resulting sequence was used to design specific primers for use in inverse PCR to amplify the entire endoPG gene of C. cubensis (ccen-1). The endoPG sequence of C. cubensis has 93% amino acid sequence similarity to that of the chestnut blight pathogen, Cryphonectria parasitica.

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Section: Discussionsupporting

confidence: 78%

Molecular Analysis of an Endopolygalacturonase Gene from a Eucalyptus Canker Pathogen, Cryphonectria cubensis

et al. 2002

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“…To amplify the fragments of the endo polygalacturonase (pg1) and exo polygalacturonase (pgx4) genes, primer sets endoF + endoR and exoF + exoR were designed based on accessioned sequences (Di Pietro and Roncero 1998;Arie et al 1998;Posada et al 2000) (Table 2). Primers were synthesized by Operon (Tokyo, Japan).…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici

Hirano

Arie

2006

J Gen Plant Pathol

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“…8, Table 9). The other two have the highest homology to two Fusarium oxysporum exopolygalacturonases [74,75].…”

Section: Glycosyl Hydrolase Family 28mentioning

confidence: 99%

The Value of Genome Sequences in the Rapid Identification of Novel Genes Encoding Specific Plant Cell Wall Degrading Enzymes

Vries¹,

Grieken²,

vanKuyk³

et al. 2005

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The potential of a genome sequence for the rapid identification of genes encoding specific enzymes was evaluated using the Aspergillus nidulans genome and plant cell wall polysaccharide degrading enzymes as an example. These enzymes are used in many industrial applications and many genes encoding these enzymes have already been identified in Aspergillus. Detailed in silica analysis of the ORFs assigned to the relevant families of the Carbohydrate Active enzyme database (CAZY, http://afmb.cnrs-mrs.fr/CAZY/index.html), using the Blast and Clustal programs, resulted in a reliable assignment of enzymatic function for most ORFs. This analysis demonstrated that approximately two-third of the A. nidulans ORFs do not yet have a characterised Aspergillus orthologue and also identified some ORFs encoding enzyme functions that have not yet been cloned in Aspergillus. A comparison of the biochemical characteristics of previously purified enzymes from A. nidulans to the A. nidulans ORFs did not result in the identification of the ORF corresponding to the enzyme activity. However, using an elimination strategy the number of candidate ORFs could be reduced to between 2 and 5.The analysis also revealed that the A. nidulans genome contains at least 33 ORFs that encode putative intracellular oligosaccharides degrading enzymes as well as ORFs with homology to oligosaccharides transporters of other organisms. This suggests that oligosaccharides are not exclusively degraded extracellularly, but can also be imported and degraded inside the cell.

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Comparative analysis of an endopolygalacturonase coding gene in isolates of seven Fusarium species

Cited by 12 publications

References 25 publications

Molecular Analysis of an Endopolygalacturonase Gene from a Eucalyptus Canker Pathogen, Cryphonectria cubensis

Molecular Analysis of an Endopolygalacturonase Gene from a Eucalyptus Canker Pathogen, Cryphonectria cubensis

PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici

The Value of Genome Sequences in the Rapid Identification of Novel Genes Encoding Specific Plant Cell Wall Degrading Enzymes

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