Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil)

Balasubramaniam, Bharathiraja; Perumal, Ayyappasamy Sudalaiyadum; Jayaraman, Jayamuthunagai; Jayakumar, M.; Ramanujam, Praveenkumar

doi:10.1016/j.wasman.2012.03.011

Cited by 48 publications

(14 citation statements)

References 24 publications

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“…A more recent example of the use of waste oil has been described by Balasubramaniam et al 132 Waste cooking oil, obtained from e.g. restaurants and canteens and mainly containing sunflower oil, was used as a substrate for biodiesel production.…”

Section: Biodiesel Productionmentioning

confidence: 99%

Immobilised enzymes in biorenewables production

et al. 2013

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Oils, fats, carbohydrates, lignin, and amino acids are all important raw materials for the production of biorenewables. These compounds already play an important role in everyday life in the form of wood, fabrics, starch, paper and rubber. Enzymatic reactions do, in principle, allow the transformation of these raw materials into biorenewables under mild and sustainable conditions. There are a few examples of processes using immobilised enzymes that are already applied on an industrial scale, such as the production of High-Fructose Corn Syrup, but these are still rather rare. Fortunately, there is a rapid expansion in the research efforts that try to improve this, driven by a combination of economic and ecological reasons. This review focusses on those efforts, by looking at attempts to use fatty acids, carbohydrates, proteins and lignin (and their building blocks), as substrates in the synthesis of biorenewables using immobilised enzymes. Therefore, many examples (390 references) from the recent literature are discussed, in which we look both at the specific reactions as well as to the methods of immobilisation of the enzymes, as the latter are shown to be a crucial factor with respect to stability and reuse. The applications of the renewables produced in this way range from building blocks for the pharmaceutical and polymer industry, transport fuels, to additives for the food industry. A critical evaluation of the relevant factors that need to be improved for large-scale use of these examples is presented in the outlook of this review.

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Section: Biodiesel Productionmentioning

confidence: 99%

Immobilised enzymes in biorenewables production

et al. 2013

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“…Hence this problem could be solved by using heterogeneous catalyst [12,27,53] and also on pretreatment with acid homogenous catalyst [18,[54][55][56] or heterogeneous catalyst [11,17,[57][58][59] to esterify the free fatty acid to form free fatty acid ester. [10,16,20,21,[32][33][34][35][36][37][38] Journal of Energy 5…”

Section: Free Fatty Acidmentioning

confidence: 99%

Recent Strategy of Biodiesel Production from Waste Cooking Oil and Process Influencing Parameters: A Review

Gnanaprakasam¹,

Sivakumar²,

Surendhar³

et al. 2013

Journal of Energy

118

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Cost of biodiesel produced from virgin vegetable oil through transesterification is higher than that of fossil fuel, because of high raw material cost. To minimize the biofuel cost, in recent days waste cooking oil was used as feedstock. Catalysts used in this process are usually acids, base, and lipase. Since lipase catalysts are much expensive, the usage of lipase in biodiesel production is limited. In most cases, NaOH is used as alkaline catalyst, because of its low cost and higher reaction rate. In the case of waste cooking oil containing high percentage of free fatty acid, alkaline catalyst reacts with free fatty acid and forms soap by saponification reaction. Also, it reduces the biodiesel conversions. In order to reduce the level of fatty acid content, waste cooking oil is pretreated with acid catalyst to undergo esterification reaction, which also requires high operating conditions. In this review paper, various parameters influencing the process of biofuel production such as reaction rate, catalyst concentration, temperature, stirrer speed, catalyst type, alcohol used, alcohol to oil ratio, free fatty acid content, and water content have been summarized.

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“…In this study, RSM was adopted to optimize the process of methanolysis of tung oil to FAME by the immobilized chimeric lipase from R. oryzae . Based on the results obtained from single factor experiments and other most commonly used factors in the transesterification reactions catalyzed by R. oryzae lipases [13,35,36], the major factors affecting the transesterfication were water content, ratio of methanol to oil, and enzyme dosage. A five-level-three-factor central composite rotatable design (CCRD) was adopted to evaluate the effects of the above three factors on the FAME yield.…”

Section: Resultsmentioning

confidence: 99%

High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Xiang

Chen

Guo

et al. 2013

Biotechnol Biofuels

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BackgroundProduction of biodiesel from non-edible oils is receiving increasing attention. Tung oil, called “China wood oil” is one kind of promising non-edible biodiesel oil in China. To our knowledge, tung oil has not been used to produce biodiesel by enzymatic method. The enzymatic production of biodiesel has been investigated extensively by using Rhizopus oryzae lipase as catalyst. However, the high cost of R. oryzae lipase remains a barrier for its industrial applications. Through different heterologous expression strategies and fermentation techniques, the highest expression level of the lipase from R. oryzae reached 1334 U/mL in Pichia pastoris, which is still not optimistic for industry applications.ResultsThe prosequence of lipases from Rhizopus sp. is very important for the folding and secretion of an active lipase. A chimeric lipase from R. oryzae was constructed by replacing the prosequence with that from the R. chinensis lipase and expressed in P. pastoris. The maximum activity of the chimera reached 4050 U/mL, which was 11 fold higher than that of the parent. The properties of the chimera were studied. The immobilized chimera was used successfully for biodiesel production from tung oil, which achieved higher FAME yield compared with the free chimeric lipase, non-chimeric lipase and mature lipase. By response surface methodology, three variables, water content, methanol to tung oil molar ratio and enzyme dosage were proved to be crucial parameters for biosynthesis of FAME and the FAME yield reached 91.9±2.5% at the optimized conditions by adding 5.66 wt.% of the initial water based on oil weight, 3.88 of methanol to tung oil molar ratio and 13.24 wt.% of enzyme concentration based on oil weight at 40°C.ConclusionsThis is the first report on improving the expression level of the lipase from R. oryzae by replacing prosequences. The immobilized chimera was used successfully for biodiesel production from tung oil. Using tung oil as non-edible raw material and a chimeric lipase from R. oryzae as an economic catalyst make this study a promising one for biodiesel applications.

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Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil)

Cited by 48 publications

References 24 publications

Immobilised enzymes in biorenewables production

Immobilised enzymes in biorenewables production

Recent Strategy of Biodiesel Production from Waste Cooking Oil and Process Influencing Parameters: A Review

High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

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