Comparative Analyses of Stilbenoids in Canes of Major Vitis vinifera L. Cultivars

Abstract

Grapevine canes are rich in resveratrol and its complex derivatives. These compounds have many biological activities and are needed mainly for health purposes. Canes, which are often wasted, can be used to produce these high-value compounds at low cost. We studied sixteen Vitis vinifera L. cultivars among the most widely cultivated ones worldwide. Polyphenols were extracted from their canes and identified by liquid chromatography-nuclear magnetic resonance spectroscopy. We accurately determined the content of E-ε-viniferin, E-resveratrol, E-piceatannol, and vitisin B and, for the first time, that of hopeaphenol and miyabenol C. The canes did not contain these major stilbene compounds in similar proportions, and their abundance and order of abundance varied according to the cultivar. For instance, Pinot noir has very high levels of E-resveratrol and E-ε-viniferin; Gewurztraminer has very high levels of vitisin B, and Carignan and Riesling have very high levels of hopeaphenol. These findings suggest that the right cultivar should be used to obtain the highest yield of a polyphenol of interest.

Varietal Distributions of Stilbenes in Grape Cane of Vitis vinifera L.

“…Contents of stilbenes are not depending only on the variety but mainly on the storing condition or the process of preparation before extracting, drying, etc. For example, content of e-viniferin in Chardonnay was found to be 2,089 ±334 mg kg -1 d.w. as published by Lambert et al (2013) which is similar to our result of 1,887 ±89 mg kg -1 as reported herein, but contents of trans-resveratrol were totally different: 190 ±87 mg kg -1 (Lambert et al, 2013) and our value was only 1,276 ±75 mg kg -1 . This big difference can be caused by various drying processes.…”

“…In the study published by Lambert et al (2013) drying occurred at the temperature of 40 °C during 15 days period but in our case there was immediately freezing and lyophilisation; in our previous study (Soural et. al., 2015) drying took place at the room temperature (22 °C approximately) for 2.5 months in darkness.…”

Biosynthetic Origin of E-Resveratrol Accumulation in Grape Canes during Postharvest Storage

“…29 Recently, pruned wood from the vineyard has been shown to accumulate a broad spectrum of stilbenoids ranging from E-resveratrol to complex stilbene oligomers. 11,28,30 Therefore, grape canes represent a promising bioresource containing valuable bioactive compounds with potential applications in medicine because of multiple pharmacological properties of stilbenoids 31 and in agriculture as an alternative source of anti-phytopathogenic substances. 12 Despite recent advances in the evaluation of stilbenoid composition of grape canes, 31−33 varying results have been reported.…”

Composition and Tissue-Specific Distribution of Stilbenoids in Grape Canes Are Affected by Downy Mildew Pressure in the Vineyard

“…32 The effective content in stilbenoids is known to be highly variable, but the determining factors influencing this composition remain poorly understood. 29,33 Although the impact of environmental cues on grape stilbenoid metabolism has been well described for leaves and berries, the influence of biotic stress during spring and summer on the stilbenoid composition of winter-pruned grape canes is unknown. In the present study, we considered the influence of downy mildew during the growing season on stilbenoid accumulation in grape canes.…”