Biosynthetic Origin of <i>E</i>-Resveratrol Accumulation in Grape Canes during Postharvest Storage

Houillé, Benjamin; Besseau, Sébastien; Courdavault, Vincent; Oudin, Audrey; Glévarec, Gaëlle; Delanoue, Guillaume; Guérin, Laurence; Simkin, Andrew J.; Papon, Nicolas; Clastre, Marc; Giglioli-Guivarc’h, Nathalie; Lanoue, Arnaud

doi:10.1021/jf505316a

Cited by 61 publications

(89 citation statements)

References 49 publications

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“…It has already been demonstrated that, after cutting, grape canes if stored properly over some months, accumulate large quantities of stilbenoid . This increase depends on storage time, temperature, relative moisture during storage, grape variety, size of cane pieces during storage, and whether the grapes were cultivated using a conventional or organic mode . However, the combined effect of pruning and storage time on the stilbene accumulation rate has not been studied before.…”

Section: Resultsmentioning

confidence: 99%

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Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

et al. 2020

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BACKGROUND: Grapevine canes represent a large source of waste derived from grape cultivation. In the present study, the effect of different processes of storage and different pruning times on the stilbene accumulation on Pinot noir canes was analyzed. Whether the alteration of the secondary metabolism accompanying leafroll symptom expressions could affect the stilbenoid accumulation in canes harvested at pruning time was also investigated.RESULTS: The maximum accumulation of trans-resveratrol and trans-piceatannol was obtained in canes harvested in October and dried at 40°C. Even in grape canes harvested in October, November, and December and stored for different times at room temperature (20 ± 2°C) a marked increase in trans-resveratrol and trans-piceatannol was evident, which reached a maximum at around 8 weeks of storage. A significant higher accumulation of trans-resveratrol and trans-piceatannol was also found in canes harvested from symptomatic plants compared to those harvested from asymptomatic plants for all the pruning times.CONCLUSION: This study confirms that the biosynthetic enzyme activities and, particularly, those involved in the stilbene pathway, persist during Pinot noir cane storage at different harvest times, with different storage times and conditions, and different sanitary status.

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Section: Resultsmentioning

confidence: 99%

“…During grape development, stilbenoids are accumulated in the whole plant and especially in woody tissues . Later, during autumn‐winter pruning, large amounts of stilbenoids can be retrieved from canes, making these byproducts a valuable source of bioactive and antioxidant compounds …”

Section: Introductionmentioning

confidence: 99%

Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

et al. 2020

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“…Stilbenes are constitutively accumulated in grape canes at the high level of about 10 g kgDW -1 (Houillé et al, 2015). Pruning results in the production each year of a very large quantity of grape canes.…”

Section: Introductionmentioning

confidence: 99%

<em>Vitis vinifera</em> canes, a source of stilbenoids against downy mildew

et al. 2016

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Aim:To investigate the antifungal efficacy of grape cane extracts enriched in stilbenes against Plasmopara viticola by in vivo experiments on grape plants. Methods and results:Experiments on grape plants were carried out from 2007 to 2008 at commercial vineyards in « Le Tourne » (southwest region of France) on Vitis vinifera L. cv Merlot plants and in a greenhouse, where 45-day-old plants (Merlot) were used. Grapevine plants were treated with stilbene extract (STE) or « Bordeaux mixture » (BM) as positive control. STE, prepared from Vitis vinifera canes, contained 33% of total stilbenes ranging from monomers to tetramers and was used at 5g L -1 . The degree of protection for downy mildew was determined as the percentage of infected leaf surface area and pathogen attack frequency. On young plants in greenhouse, P. viticola inoculation and three treatments with STE were carried out. After the treatments, the disease reduction was from 59 to 69% for pathogen attack frequency and from 83 to 88% for infected leaf surface for the two modes of infestation (artificial and secondary). STE triggered a similar intense decrease in downy mildew infection as recorded for BM. In the vineyard assay, downy mildew infection was allowed to occur naturally. Field grown plants were treated every 7 th day for eight weeks or every 14 th day for eight weeks during June and July. We observed that STE was also able to prevent the infection of P. viticola in vineyard but to a lesser extent. Treatment with STE every 7 th day provided better results in terms of infection frequency and leaf area than that every 14 th day. STE treatment of plants every 7 th day in vineyard reduced the frequency of attack by 16 to 39% and leaf surface infection by 57 to 61%, which was lower than that recorded for BM. STE also displayed in vitro inhibitory effects on the release of zoospores from sporangia and the germination of zoospores of P. viticola. Zoospore germination was completely inhibited at a concentration of 100 mg L -1 and zoospore release was inhibited at 330 mg L -1 . Conclusions:Treatment of young grapevine plants in greenhouse with stilbene extract from grapevine canes offered considerable protection against P. viticola that was identical to that provided by Bordeaux mixture. STE was also able to prevent the infection of P. viticola in vineyard but to a lesser extent. Treatment with STE every 7 th day in vineyard provided better results than that every 14 th day.Significance and impact of the study: Stilbenes from grapevine canes are good candidates as natural fungicides against downy mildew. Given the very large quantity of grape canes available each year, this strategy could be scaled up to control this grapevine disease in a sustainable manner.Abstract manuscript

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“…20 Comparative studies on the spatial repartition of stilbenoids in young grape plants showed that resveratrol accumulation is predominant in stems 27 and that its accumulation herein persists after grape harvest. 28,29 Therefore, winter pruning of grape vine represents an annual step producing stilbenoid-rich wastes. The important volume of pruned wood (1−5 tons/hectare/year) and the world's grape growing surface (7.5 million hectares) make the grape canes a prominent source of stilbenoids.…”

Section: ■ Introductionmentioning

confidence: 99%

“…32 The effective content in stilbenoids is known to be highly variable, but the determining factors influencing this composition remain poorly understood. 29,33 Although the impact of environmental cues on grape stilbenoid metabolism has been well described for leaves and berries, the influence of biotic stress during spring and summer on the stilbenoid composition of winter-pruned grape canes is unknown. In the present study, we considered the influence of downy mildew during the growing season on stilbenoid accumulation in grape canes.…”

Section: ■ Introductionmentioning

confidence: 99%

Composition and Tissue-Specific Distribution of Stilbenoids in Grape Canes Are Affected by Downy Mildew Pressure in the Vineyard

Houillé

Besseau

Delanoue

et al. 2015

J. Agric. Food Chem.

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Grape canes are byproducts of viticulture containing valuable bioactive stilbenoids including monomers and oligomers of E-resveratrol. Although effective contents in stilbenoids are known to be highly variable, the determining factors influencing this composition remain poorly understood. As stilbenoids are locally induced defense compounds in response to phytopathogens, this study assessed the impact of downy mildew infection during the growing season on the stilbenoid composition of winter-harvested grape canes. The spatial distribution between pith, conducting tissues, and cortex of E-piceatannol, E-resveratrol, E-ε-viniferin, ampelopsin A, E-miyabenol C, Z/E-vitisin B, hopeaphenol, and isohopeaphenol in grape canes from infected vineyards was strongly altered. In conducting tissues, representing the main site of stilbenoid accumulation, E-ε-viniferin content was higher and E-resveratrol content was lower. These findings suppose that the health status in vineyards could modify the composition of stilbenoids in winter-harvested grape canes and subsequently the potential biological properties of the valuable extracts.

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Biosynthetic Origin of E-Resveratrol Accumulation in Grape Canes during Postharvest Storage

Cited by 61 publications

References 49 publications

Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

<em>Vitis vinifera</em> canes, a source of stilbenoids against downy mildew

Composition and Tissue-Specific Distribution of Stilbenoids in Grape Canes Are Affected by Downy Mildew Pressure in the Vineyard

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