Comparación histoquímica de albura y duramen de tres especies de Quercus

Abstract

Histochemical and some quantitative features of sapwood and heartwood cells of Quercus glabrescens, Q. potosina y Q. eduardii are described. The trees were collected in different sites of Huayacocotla, in Veracruz State. The results show that sapwood of the species are very similar in histochemical and quantitative trends but heartwood differ on chemical deposits of gelatinous fibers, and violet deposits of polisacharids present on rays and also the petrification processes in Q. potosina rays. Cell wall quality and the relationship between dimensions and types of chemical compounds are discussed as well.

Histoquímica, contenido de fenoles totales y actividad antioxidante de hoja y de madera de Litsea glaucescens Kunth (Lauraceae)

“…En los cortes de las hojas y la madera se detectó la lignina (color rojo) empleando el reactivo de Wiesner (fluorogluci-nol al 2% en etanol al 96%) por cinco minutos en secciones frescas y montando con una gota de HCl 6N (Rivera et al, 1999). También se usó el reactivo de Maüle (solución acuosa de permanganato de potasio al 1%) por cinco minutos en secciones frescas.…”

“…Para la detección y localización de los taninos, se empleó la técnica de vainillina-HCl (solución saturada de vainillina en etanol al 95%), se cubrieron secciones frescas durante 5 minutos y montando con una gota de HCl 9N (naranja rojizo) (Rivera et al, 1999).…”

“…Asimismo se empleó Lugol para contrastar lignina (amarillo) de carbohidratos no estructurales (morado azulado) (Rivera et al, 1999).…”

Histology of <em>Austrocedrus chilensis</em> roots during infection by <em>Phytophthora austrocedri</em>

“…For the visualization of structures in the non-inoculated healthy plants, it was used the safraninfast green staining following Ma et al (1992) [10] with modifications; or no staining and direct mounting in water of the sections. Lugol was used to contrast lignin (yellow) to non-structural carbohydrates, mainly starch (bluish to black) [11]. Toluidine blue was used to contrast lignin and polyphenols (turquoise to green), carbohydrates (lilac color) and oils (no color) [11].…”

“…Lugol was used to contrast lignin (yellow) to non-structural carbohydrates, mainly starch (bluish to black) [11]. Toluidine blue was used to contrast lignin and polyphenols (turquoise to green), carbohydrates (lilac color) and oils (no color) [11]. Lignin detection was carried out according to the methodology described by Mauch-Mani and Slusarenko (1996) [12].…”