Community structure and diversity of endophytic bacteria in seeds of three consecutive generations of Crotalaria pumila growing on metal mine residues

Sánchez-López, Ariadna S.; Thijs, Sofie; Beckers, Bram; González-Chávez, Ma. del Carmen A.; Weyens, Nele; Carrillo-González, Rogelio; Vangronsveld, Jaco

doi:10.1007/s11104-017-3176-2

Cited by 72 publications

(45 citation statements)

References 62 publications

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“…Staphylococcus isolates were, however, recently found consistently associated with plant tissues (Ali et al, 2010), including seeds (Alibrandi et al, 2017;Sánchez-López et al, 2018). Our isolates showed indeed a considerable tolerance to a wide range of abiotic stresses typical of the coastal mangrove environment, like high temperature, osmotic stress and high NaCl concentration, indicating that they are adaptated to this specific habitat.…”

Section: Discussionsupporting

confidence: 49%

Bacterial endophytes of mangrove propagules elicit early establishment of the natural host and promote growth of cereal crops under salt stress

Soldan

Mapelli

Crotti

et al. 2019

Microbiological Research

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Mangroves, dominating tropical intertidal zones and estuaries, are among the most salt tolerant plants, and propagate through reproductive units called propagules. Similarly to other plants' seeds, propagules may harbor beneficial bacteria. Our hypothesis was that mangroves, being able to grow into seawater, should harbor bacteria able to interact with the host and to exert positive effects under salt stress, which could be exploited to improve crop production. Therefore, we isolated bacterial endophytes from mangrove propagules with the aim to test whether these bacteria have a beneficial potential on their natural host and on different crops like barley and rice, cultivated under salt stress. The 172 bacterial isolates obtained were screened for plant growth promotion (PGP) activities in vitro, and the 12 most promising isolates were tested on barley under non-axenic conditions and salt stress. Gordonia terrae KMP456-M40 was the best performing isolate, increasing ear weight by 65%. Basing on the in vivo PGP activity and the root colonization ability, investigated by fluorescence in situ hybridization and confocal microscopy, three strains were additionally tested on mangrove propagule germination and on rice growth. The most effective strain was again G. terrae KMP456-M40, which enhanced the root length of mangrove seedlings and the biomass of salt-stressed rice under axenic conditions up to 65% and 62%, respectively. We demonstrated that propagules, the reproductive units of mangroves, host beneficial bacteria that enhance the potential of mangrove seedlings establishment and confer salt tolerance to cereal crops.

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Section: Discussionsupporting

confidence: 49%

Bacterial endophytes of mangrove propagules elicit early establishment of the natural host and promote growth of cereal crops under salt stress

Soldan

Mapelli

Crotti

et al. 2019

Microbiological Research

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“…The definition of the "core endomicrobiome" is variable within the literature with some research groups defining it as the OTUs present in at least 50% of the samples, with others at 90%. A study on the seeds of Crotalaria pumila revealed Methylobacterium as the dominant OTU and constituted more than 80% of the core microbiome [26]. The genus Pseudomonas is ubiquitous in nature and part of the core endomicrobiome of many plants ranging from model plants like Arabidopsis thaliana to medicinal plants like Cannabis sativa [27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Community Structure, Diversity and Potential of Endophytic Bacteria in the Primitive New Zealand Medicinal Plant Pseudowintera colorata

Purushotham

Jones

Monk

et al. 2020

Plants

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Although the importance of the plant microbiome in commercial plant health has been well established, there are limited studies in native medicinal plants. Pseudowintera colorata (horopito) is a native New Zealand medicinal plant recognized for its antimicrobial properties. Denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq analysis of P. colorata plants from ten sites across New Zealand showed that tissue type strongly influenced the diversity and richness of endophytic bacteria (PERMANOVA, P < 0.05). In addition, two OTUs belonging to the genus Pseudomonas (Greengenes ID: 646549 and 138914) were found to be present in >75% of all P. colorata leaf, stem and root samples and were identified as the members of the P. colorata “core endomicrobiome”. Culture-independent analysis was complemented by the recovery of 405 endophytic bacteria from the tissues of P. colorata. Some of these cultured endophytic bacteria (n = 10) showed high antagonism against four different phytopathogenic fungi tested. The influence of endophytic bacteria on plant growth was assessed by inoculating P. colorata seedlings. The mean shoot height of seedlings treated with Bacillus sp. TP1LA1B were longer (1.83×), had higher shoot dry weight (1.8×) and produced more internodes (1.8×) compared to the control.

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“…(), Johnston‐Monje and Raizada () and Sanchez‐Lopez et al. (). Highly interesting results from their study also showed that the mature second generation seeds had bacterial endophytic populations that showed a strikingly similar pattern of the community as the first generation seeds.…”

Section: Discussionmentioning

confidence: 99%

“…Potentially the most intriguing characteristic of seed bacterial endophytes is their vertical transmission and conservation into the next generation plants (Johnston‐Monje & Raizada, ; Truyens, Weyens, Cuypers, & Vangronsveld, ). Consecutive seed generations consistently show similar endophytic communities with prominent dominant groups (Sanchez‐Lopez et al., ). Additionally, the potential existence of core seed bacterial endophytes was also observed in Oryza sativa ssp.…”

Section: Introductionmentioning

confidence: 94%

Conservation and transmission of seed bacterial endophytes across generations following crossbreeding and repeated inbreeding of rice at different geographic locations

et al. 2018

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There are comparatively diverse bacterial communities inside seeds, which are vertically transmitted and conserved, becoming sources of endophytes in the next generation of host plants. We studied how rice seed endophyte composition changed over time following crossbreeding, repeated inbreeding, subsequent human selection and planting of different rice seeds in different ecogeographical locations. Using terminal-restriction fragment length polymorphism analysis to study bacterial communities, we observed that diversity between the original parents and their offspring may show significant differences in richness, evenness and diversity indices. Heat maps reveal substantial contributions of both or either parent in the shaping of the bacterial seed endophytes of the offspring. Most of the terminal restriction fragments (T-RFs) of the subsequent progeny could be traced to any or both of its parents while unique T-RFs of the offspring suggest external sources of colonization particularly when the seeds were cultivated in different locations. Many similar groups of endophytic bacteria persist in the seeds even after recultivation in different locations, indicating resilience to environmental changes and conservation of bacteria across generations. This study suggests that parent plants contributed to the shaping of seed bacterial endophytes of their offspring, although it is also possible that these soil grown rice plants recruit similar populations of endophytes from the soil generation after generation. This study also highlights some bacterial groups belonging to Herbaspirillum, Microbacterium, Curtobacterium, Stenotrophomonas, Xanthomonas and Enterobacter that may be part of a transmitted and conserved "core microbiota" that are ubiquitous and dominant members of the endophytic communities of the rice seeds.

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Community structure and diversity of endophytic bacteria in seeds of three consecutive generations of Crotalaria pumila growing on metal mine residues

Cited by 72 publications

References 62 publications

Bacterial endophytes of mangrove propagules elicit early establishment of the natural host and promote growth of cereal crops under salt stress

Bacterial endophytes of mangrove propagules elicit early establishment of the natural host and promote growth of cereal crops under salt stress

Community Structure, Diversity and Potential of Endophytic Bacteria in the Primitive New Zealand Medicinal Plant Pseudowintera colorata

Conservation and transmission of seed bacterial endophytes across generations following crossbreeding and repeated inbreeding of rice at different geographic locations

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