Community Structure, Diversity and Potential of Endophytic Bacteria in the Primitive New Zealand Medicinal Plant Pseudowintera colorata

Abstract

Although the importance of the plant microbiome in commercial plant health has been well established, there are limited studies in native medicinal plants. Pseudowintera colorata (horopito) is a native New Zealand medicinal plant recognized for its antimicrobial properties. Denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq analysis of P. colorata plants from ten sites across New Zealand showed that tissue type strongly influenced the diversity and richness of endophytic bacteria (PERMANOVA, P < 0.05). In addition, two OTUs belonging to the genus Pseudomonas (Greengenes ID: 646549 and 138914) were found to be present in >75% of all P. colorata leaf, stem and root samples and were identified as the members of the P. colorata “core endomicrobiome”. Culture-independent analysis was complemented by the recovery of 405 endophytic bacteria from the tissues of P. colorata. Some of these cultured endophytic bacteria (n = 10) showed high antagonism against four different phytopathogenic fungi tested. The influence of endophytic bacteria on plant growth was assessed by inoculating P. colorata seedlings. The mean shoot height of seedlings treated with Bacillus sp. TP1LA1B were longer (1.83×), had higher shoot dry weight (1.8×) and produced more internodes (1.8×) compared to the control.

Arnebia euchroma, a Plant Species of Cold Desert in the Himalayas, Harbors Beneficial Cultivable Endophytes in Roots and Leaves

“…This was true for both bacterial and fungal endophytic microbiota (Table 1). These results corroborate with the study of Purushotham et al (2020) where the richness of endophytic Alpha, Beta, and Gammaproteobacteria was higher in leaves in comparison to stem and roots of primitive New Zealand medicinal plant Pseudowintera colorata. Fernandes et al (2015) and Sharma et al (2018) also observed higher diversity and greater species richness for fungal endophytes in leaf as compared to other plant tissues in Glycine max and Berberis aristata DC.…”

Bioprospecting Beneficial Endophytic Bacterial Communities Associated With Rosmarinus Officinalis for Alleviating Plant Productivity

“…Among all, the strains of Bacillus, Pseudomonas and Cedecea exhibited the highest amounts of Psolubilization, siderophore, IAA, HCN, ammonia and lytic enzymes (chitinase, protease, and amylase). Earlier studies have also indicated that several Bacillus species isolated from medicinal plants produced phytohormones, solubilized P and improved growth of tomato (Abdelshafy Mohamad et al 2020) and Pseudowintera colorata (Purushotham et al 2020). Similarly, Pseudomonas species produced IAA and increased plant biomass of medicinal plant Astragalus mongholicus (Sun et al 2019).…”

Endophytic nitrogen-fixing bacteria DX120E inoculation altered the carbon and nitrogen metabolism in sugarcane

“…The difference in numbers and types of carbohydrates and amino acids enriched in leaves and stems of sugarcane indicated that the variety B8 was stronger than ROC22, 10.3389/fmicb.2022.1000033 Frontiers in Microbiology 13 frontiersin.org and leaves were stronger stems. Changes in metabolites and metabolic pathways might the bacterial penetration in the root cell wall for their host (Purushotham et al, 2020) and decrease external N requirements. So, it was found that endophytic N-fixing bacteria has an essential role in carbon and N metabolism and improvement of sugarcane growth.…”