Community spread of extended-spectrum β-lactamase-producing bacteria detected in social insurance hospitals throughout Japan

Shibasaki, Mayumi; Komatsu, Masaru; Sueyoshi, Noriyuki; Maeda, Misaho; Uchida, Takae; Yonezawa, H.; Inagaki, Kenji; Omi, Ayako; Matsumoto, Hidenobu; Murotani, Makiko; Iwamoto, Tsukasa; Kodaka, Yoshihiro; Kieda, Hideto; Tokiwa, Manabu; Masuwa, Bunji; Kinoshita, Mari; Saito, K; Katou, Masahiko

doi:10.1016/j.jiac.2016.03.001

Cited by 22 publications

(14 citation statements)

References 16 publications

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“…The CTX‐M‐9 dominance of the ESBL genotype in the present cohort (63.6%) was comparable with recent data in Japan of patients with various infectious diseases (53.0–77.0%) and healthy food handling labors (56.6%) . CTX‐M‐1, which is dominant in Europe, was detected in 36.3%, and CTX‐M‐2 was found in none of the men.…”

Section: Discussionsupporting

confidence: 89%

Prevalence and characteristics of fecal antimicrobial‐resistant Escherichia coli in a cohort of Japanese men undergoing prostate biopsy

et al. 2017

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The present study showed the fecal carriage of fluoroquinolone-resistant E. coli and extended-spectrum β-lactamase-producing E. coli at 9.8% and 5.9%, respectively, with CTX-M-9 group of extended-spectrum β-lactamase-producing E. coli comprising 63.6%, in Japanese men receiving prostate biopsy. The carriage of fluoroquinolone-resistant and/or extended-spectrum β-lactamase-producing E. coli was significantly related to diabetes.

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Section: Discussionsupporting

confidence: 89%

Prevalence and characteristics of fecal antimicrobial‐resistant Escherichia coli in a cohort of Japanese men undergoing prostate biopsy

et al. 2017

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“…CTX-M-27 and CTX-M-14 genes detected in this study belong to the group of CTX-M-9 (Bonnet, 2004), which is the predominate type in Japan, especially in nosocomial infections (Hawkey and Jones, 2009; Matsumura et al, 2015; Shibasaki et al, 2016). When any infections occur in the hospital, the analysis of CTX-M gene is important for the detection of the bacterial origin and route of transmission.…”

Section: Discussionmentioning

confidence: 63%

Comprehensive Diagnosis of Bacterial Infection Associated with Acute Cholecystitis Using Metagenomic Approach

et al. 2017

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Acute cholecystitis (AC), which is strongly associated with retrograde bacterial infection, is an inflammatory disease that can be fatal if inappropriately treated. Currently, bacterial culture testing, which is basically recommended to detect the etiological agent, is a time-consuming (4–6 days), non-comprehensive approach. To rapidly detect a potential pathogen and predict its antimicrobial susceptibility, we undertook a metagenomic approach to characterize the bacterial infection associated with AC. Six patients (P1–P6) who underwent cholecystectomy for AC were enrolled in this study. Metagenome analysis demonstrated possible single or multiple bacterial infections in four patients (P1, P2, P3, and P4) with 24-h experimental procedures; in addition, the CTX-M extended-spectrum ß-lactamase (ESBL) gene was identified in two bile samples (P1 and P4). Further whole genome sequencing of Escherichia coli isolates suggested that CTX-M-27-producing ST131 and CTX-M-14-producing novel-ST were identified in P1 and P4, respectively. Metagenome analysis of feces and saliva also suggested some imbalance in the microbiota for more comprehensive assessment of patients with AC. In conclusion, metagenome analysis was useful for rapid bacterial diagnostics, including assessing potential antimicrobial susceptibility, in patients with AC.

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“…В Испании частота де текции генов кластера bla CTXM9 у E. coli, колонизирующих слизистую оболочку кишечника больных с гематологиче скими заболеваниями, составила 55% [20], в нашем ис следовании -только у 14,8% Enterobacterales. В Японии гены кластера bla CTXM9 преобладали у Enterobacterales, выделенных из гемокультуры (43,2%) [21], и среди изо лятов, вызвавших внебольничные инфекции (66,9%) [22]. Только один (0,2%) из исследуемых нами изолятов Enterobacterales содержал в своем геноме ген кластера bla CTXM2 .…”

Section: Discussionunclassified

“…Том 20 . №4 АНТИБИОТИКОРЕЗИСТЕНТНОСТЬ кластера bla CTXM2 также была невысокой и варьировала от полного отсутствия до 713,4% [3,20,22].…”

Section: Discussionunclassified

Molecular characterization of extended-spectrum beta-lactamase-producing Enterobacterales isolates collected from blood culture in patients with hematological malignancies

Khrulnova¹,

Коробова²,

Fyodorova³

et al. 2018

CMAC

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Цель. Изучить распределение генов blaCTXM, blaTEM и blaSHV у изолятов Enterobacterales с продукци ей беталактамаз расширенного спектра (БЛРС), выделенных из гемокультуры больных опухолями системы крови. Материалы и методы. Материалом исследования были изоляты Enterobacterales, выделенные из гемокультуры больных, находившихся на стационарном лечении в 10 лечебных учреждениях России (20032015 гг.). Продукция БЛРС у Enterobacterales была определена фенотипическими методами (CLSI 2017). Наличие генов, кодирующих ферменты TEM, SHV и CTXM, определяли методом поли меразной цепной реакции (ПЦР) с использованием специфических праймеров. Результаты. Всего было исследовано 499 изолятов Enterobacterales с продукцией БЛРС. В структуре микроорганизмов преобладали Klebsiella pneumoniae (43,9%) и Escherichia coli (43,3%). Исследуе мые гены были представлены у Enterobacterales как в сочетании (79%), так и изолированно (19,6%). Гены blaCTXM были наиболее распространенными (82,6%), далее следовали гены blaTEM (73,7%) и blaSHV (53,7%). У 1,4% (n=7) изолятов Enterobacterales с продукцией БЛРС исследуемые гены blaCTXM, blaTEM, blaSHV выявлены не были. Гены blaCTXM были обнаружены у 84,9% K. pneumoniae, 89,8% E. coli и 42,6% Enterobacter cloacae. Гены blaCTXM принадлежали к кластерам blaCTXM1 (88,8%), blaCTXM9 (14,8%) и blaCTXM2 (0,2%). Доля изолятов, имеющих сочетание генов двух разных кластеров blaCTXM, составила 4,1%. Выводы. Среди Enterobacterales с продукцией БЛРС наиболее распространенными были гены blaCTXM (82,6%), основная доля которых принадлежала к кластеру blaCTXM1 (88,8%). У большинства исследуемых изолятов (79%) было выявлено сочетание генов разных типов беталактамаз, а у 4,1% CTXMпродуцирующих Enterobacterales-сочетание генов двух разных кластеров.

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Community spread of extended-spectrum β-lactamase-producing bacteria detected in social insurance hospitals throughout Japan

Cited by 22 publications

References 16 publications

Prevalence and characteristics of fecal antimicrobial‐resistant Escherichia coli in a cohort of Japanese men undergoing prostate biopsy

Prevalence and characteristics of fecal antimicrobial‐resistant Escherichia coli in a cohort of Japanese men undergoing prostate biopsy

Comprehensive Diagnosis of Bacterial Infection Associated with Acute Cholecystitis Using Metagenomic Approach

Molecular characterization of extended-spectrum beta-lactamase-producing Enterobacterales isolates collected from blood culture in patients with hematological malignancies

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