Communication—Scanning Electrochemical Microscopy Analysis of Interleukin-6 in Oral Cancer

Abstract

Scanning electrochemical microscopy (SECM) offers a highly sensitive and convenient approach for electrochemical mapping of interleukin-6 (IL-6), an oral cancer protein biomarker. In this study, we utilized the SECM to map the distribution of IL-6 for imaging different cancer stages on the tissue sections. The SECM results indicated that IL-6 was expressed higher in cancer tissues as compared to normal tissue and hence, SECM can be employed to improve oral cancer diagnosis. Also, IL-6 analysis can be beneficial for predicting the severity of COVID-19 at an early stage to eliminate the potential risks of serious respiratory complications in affected patients.

AI-Assisted Fusion of Scanning Electrochemical Microscopy Images Using Novel Soft Probe

“…To explore more facts about IL-6 distribution and compare the stability of the novel gold ultramicroelectrode, we also investigated more SECM images and x line scans by carbon soft stylus microelectrode used in the previous study (figure S8). 11 The sensitivity and quality of SECM images or line scans performed by the commercial soft carbon stylus electrode and newly developed electrode were similar. The results of line scan experiments were comparable.…”

“…Finally, the slices were immersed in secondary antibodies -HRP (200 times diluted in PBS), incubated for 1 hour at RT, and washed with Triton X-100 and PBS as in the previous step. 11,39,40…”

“…[3][4][5][6][7] Moreover, scanning probe techniques combined with optical images can offer more information that allows scientists to understand chemical reactions during the catalytic processes and kinetics of certain chemical reactions. [8][9][10][11][12][13][14][15][16] Nevertheless, the quality of SECM images usually depends on the electrode sizes of the electrodes and many uncontrollable factors. [17][18][19][20][21][22] For example, manipulating the fragile glass ultramicroelectrodes and blurred images sometimes frustrates researchers.…”

AI-Assisted Fusion of Scanning Electrochemical Microscopy Images Using Novel Soft Probe

“…To explore more facts about the distribution of IL-6 and compare the stability of the new gold ultramicroelectrode, we also investigated more SECM images and x-line scans using the carbon soft stylus microelectrode used in the previous study (Figure S10). 11 The sensitivity and quality of SECM images or line scans performed by the commercial soft carbon stylus electrode and the newly developed electrode were similar. The results of the line scan experiments were comparable.…”

“…Finally, the slices were immersed in secondary antibodies�HRP (200 times diluted in PBS), incubated for 1 h at RT, and washed with Triton X-100 and PBS as in the previous step. 11,39,40…”