Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins

Gilbert, Catherine E; Sztul, Elizabeth; Machamer, Carolyn E.

doi:10.1091/mbc.e17-11-0622

Cited by 18 publications

(14 citation statements)

References 57 publications

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“…Several studies have shown that reduced temperature blocks the particular intracellular steps of protein trafficking through the ER-Golgi-PM pathway (Saraste et al 1986;Kuismanen and Saraste 1989;Presley et al 1997). In mammalian cells, transition vesicles continue to be formed at temperatures below 20°C but their migration to the Golgi apparatus is prevented (Gilbert et al 2018). This temperatureinduced transport block has been developed as a tool to analyze the membrane traffic (Kuismanen and Saraste 1989).…”

Section: Discussionmentioning

confidence: 99%

Florigen trafficking integrates photoperiod and temperature signals in Arabidopsis

Liu

2020

JIPB

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The transition to flowering is the most dramatic phase change in flowering plants and is crucial for reproductive success. A complex regulatory network in plants has evolved to perceive and integrate the endogenous and environmental signals. These signals perceived, including day length and temperature, converge to regulate FLOWERING LOCUS T (FT), which encodes a mobile stimulus required for floral induction in Arabidopsis. Despite the discovery of modulation of FT messenger RNA (mRNA) expression by ambient temperature, whether the trafficking of FT protein is controlled in response to changes in growth temperature is so far unknown. Here, we show that FT transport from companion cells to sieve elements is controlled in a temperature‐dependent manner. This process is mediated by multiple C2 domain and transmembrane region proteins (MCTPs) and a soluble N‐ethylmaleimide‐sensitive factor protein attachment protein receptor (SNARE). Our findings suggest that ambient temperatures regulate both FT mRNA expression and FT protein trafficking to prevent precocious flowering at low temperatures and ensure plant reproductive success under favorable environmental conditions.

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Section: Discussionmentioning

confidence: 99%

Florigen trafficking integrates photoperiod and temperature signals in Arabidopsis

Liu

2020

JIPB

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“…ARFs also recruit non-coat proteins to membranes (e.g., golgin-160, GCC88) (Derby et al. , 2004; Gilbert et al. , 2018) and can activate lipid-modifying enzymes (e.g., phospholipase D, PI(4) 5-kinase) (Brown et al.…”

Section: Arf Family Gtpasesmentioning

confidence: 99%

ARF GTPases and their GEFs and GAPs: concepts and challenges

Sztul

Chen

Casanova

et al. 2019

MBoC

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196

312

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Detailed structural, biochemical, cell biological, and genetic studies of any gene/protein are required to develop models of its actions in cells. Studying a protein family in the aggregate yields additional information, as one can include analyses of their coevolution, acquisition or loss of functionalities, structural pliability, and the emergence of shared or variations in molecular mechanisms. An even richer understanding of cell biology can be achieved through evaluating functionally linked protein families. In this review, we summarize current knowledge of three protein families: the ARF GTPases, the guanine nucleotide exchange factors (ARF GEFs) that activate them, and the GTPase-activating proteins (ARF GAPs) that have the ability to both propagate and terminate signaling. However, despite decades of scrutiny, our understanding of how these essential proteins function in cells remains fragmentary. We believe that the inherent complexity of ARF signaling and its regulation by GEFs and GAPs will require the concerted effort of many laboratories working together, ideally within a consortium to optimally pool information and resources. The collaborative study of these three functionally connected families (≥70 mammalian genes) will yield transformative insights into regulation of cell signaling.

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“…The cell concentration of each group was adjusted to 1.0×10 3 , and then the cells were inoculated into a 96-well plate. Each well was added RPMI-1640 medium containing 10% FBS to 200μl, and the cells were cultured in 5% CO2 incubator at 37°C.…”

Section: Mtt Assaymentioning

confidence: 99%

“…As a member of the Ras gene superfamily, Arf can allosterically activate cholera toxin, enhance its ADP-ribosyl transferase activity, and promote its ADP-ribosylation on Gs protein α subunit. Arf6 is the most specific subtype of Arfs protein family with a molecular weight of about 20 kD, playing an important role in regulating endocytosis, extracellular secretion, endocytic membrane circulation, cell division, membrane lipid metabolism, microcapsule transport and release, formation of intercellular adhesion and junction, as well as cortical actin cytoskeletal remodeling (3). In addition, Arf6 is associated with membrane receptor endocytosis in clathrin-dependent and independent pathways (4,5).…”

Section: Introductionmentioning

confidence: 99%

Effects of Arf6 downregulation on biological characteristics of human prostate cancer cells

Lei

Jia

et al. 2020

Int. braz j urol.

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Objective: To evaluate the effects of Arf6 downregulation on human prostate cancer cells. Materials and Methods: The effects of Arf6 downregulation on cell proliferation, migration, invasion and apoptosis were assessed by MTT, BrdU, scratch, Transwell assays and flow cytometry respectively. AKT, p-AKT, ERK1/2, p-ERK1/2 and Rac1 protein expressions were detected by Western blot. Results: Downregulating Arf6 by siRNA interference suppressed the mRNA and protein expressions of Arf6. The proliferation capacities of siRNA group at 48h, 72h, and 96h were significantly lower than those of control group (P <0.05). The migration distance of siRNA group at 18h was significantly shorter than that of control group (P <0.01). The number of cells penetrating Transwell chamber membrane significantly decreased in siRNA group compared with that of control group (P <0.01). After 24h, negative control and normal control groups had similar apoptotic rates (P >0.05) which were both significantly lower than that of siRNA group (P <0.01). After Arf6 expression was downregulated, p-ERK1/2 and Rac1 protein expressions were significantly lower than those of control group (P <0.05). Conclusion: Downregulating Arf6 expression can inhibit the proliferation, migration and invasion of prostate cancer cells in vitro, which may be related to ERK1/2 phosphorylation and Rac1 downregulation. ARTICLE INFO Bo Tan

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Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins

Cited by 18 publications

References 57 publications

Florigen trafficking integrates photoperiod and temperature signals in Arabidopsis

Florigen trafficking integrates photoperiod and temperature signals in Arabidopsis

ARF GTPases and their GEFs and GAPs: concepts and challenges

Effects of Arf6 downregulation on biological characteristics of human prostate cancer cells

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