Common Tasks in Microscopic and Ultrastructural Image Analysis Using ImageJ

Papadopulos, Francesca Marzia; Spinelli, Matthew A; Valente, Sabrina; Foroni, Laura; Orrico, Catia; Alviano, Francesco; Pasquinelli, Gianandrea

doi:10.1080/01913120701719189

Cited by 201 publications

(118 citation statements)

References 10 publications

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“…Ten random areas from each section were photographed using digital camera. Then, cross-sectional areas of the muscle fibers were measured using ImageJ software as suggested previously (Papadopulos et al, 2007;Minamoto et al, 2007). After that, the average muscle fiber cross-sectional area was calculated for each animal in each group.…”

Section: Muscle Loss Assessmentmentioning

confidence: 99%

Apoptotic Mediators are Upregulated in the Skeletal Muscle of Chronic/Progressive Mouse Model of Parkinson's Disease

Erekat

2015

The Anatomical Record

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Apoptosis has been implicated in the pathogenesis of Parkinson disease (PD). Parkinson disease is characterized by skeletal muscle abnormalities. The aim of this study is to illustrate the impact of PD induction on the expression of apoptotic mediators. Twenty normal albino mice were randomly selected and equally divided in control and PD groups. Chronic Parkinsonism was induced in the PD group using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and probenecid (MPTP/p). After that, samples from gastrocnemius muscles were evaluated by immunohistochemistry to examine the expression of p53 and active caspase-3 in the two groups of animals. P53 and active caspase-3 expression was significantly higher in gastrocnemius skeletal muscle in PD mice compared with that in the control mice (P value <0.01). Furthermore, we show PD gastrocnemius muscle atrophy measured by significant reduction (P < 0.01) in the muscle fiber cross-sectional area. Thus, our present data suggest that PD induction increased the expression of the apoptotic mediators p53 and active caspase-3 in gastrocnemius muscle, indicating the induction of apoptosis, which was correlative with gastrocnemius muscle atrophy subsequent to the induction of PD.

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Section: Muscle Loss Assessmentmentioning

confidence: 99%

Apoptotic Mediators are Upregulated in the Skeletal Muscle of Chronic/Progressive Mouse Model of Parkinson's Disease

Erekat

2015

The Anatomical Record

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“…Length of exposure was optimized to avoid saturation of silver grains in any of the incubations. Quantification of silver grain density on Thioploca cells was done by image analysis, using the Image J software (Papadopulos et al, 2007).…”

Section: Quantitative Microautoradiographymentioning

confidence: 99%

Physiology and behaviour of marine Thioploca

et al. 2009

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Among prokaryotes, the large vacuolated marine sulphur bacteria are unique in their ability to store, transport and metabolize significant quantities of sulphur, nitrogen, phosphorus and carbon compounds. In this study, unresolved questions of metabolism, storage management and behaviour were addressed in laboratory experiments with Thioploca species collected on the continental shelf off Chile. The Thioploca cells had an aerobic metabolism with a potential oxygen uptake rate of 1760 lmol O 2 per dm 3 biovolume per h, equivalent to 4.4 nmol O 2 per min per mg protein. When high ambient sulphide concentrations (B200 lM) were present, a sulphide uptake of 6220 ± 2230 lmol H 2 S per dm 3 per h, (mean ± s.e.m., n ¼ 4) was measured. This sulphide uptake rate was six times higher than the oxidation rate of elemental sulphur by oxygen or nitrate, thus indicating a rapid sulphur accumulation by Thioploca. Thioploca reduce nitrate to ammonium and we found that dinitrogen was not produced, neither through denitrification nor through anammox activity. Unexpectedly, polyphosphate storage was not detectable by microautoradiography in physiological assays or by staining and microscopy. Carbon dioxide fixation increased when nitrate and nitrite were externally available and when organic carbon was added to incubations. Sulphide addition did not increase carbon dioxide fixation, indicating that Thioploca use excess of sulphide to rapidly accumulate sulphur rather than to accelerate growth. This is interpreted as an adaptation to infrequent high sulphate reduction rates in the seabed. The physiology and behaviour of Thioploca are summarized and the adaptations to an environment, dominated by infrequent oxygen availability and periods of high sulphide abundance, are discussed.

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“…SEM backscattered images were first filtered to reduce the noise and then converted to binary image by threshold operation to see the area covered by gold particles (in black) and that of the substrate (in white). Then, ImageJ can measure this covering [10]. The ITO example is shown in Fig.…”

Section: Xps and Sem Experimental Results Analysis Processmentioning

confidence: 99%

“…Because the average atomic number of gold is far from that of TCO atoms, the backscattering mode allowed us to obtain well-contrasted pictures for further treatment by ImageJ software. This last one was used to estimate the rate coverage of the TCO films by the gold nanodots [10]. Only the powerful resolution of this field emission SEM with its large-angle backscattered detector allowed us to obtain images at a very high magnification in backscattered mode.…”

Section: Methodsmentioning

confidence: 99%

On the ultrathin gold film used as buffer layer at the transparent conductive anode/organic electron donor interface

et al. 2011

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Previously, we have shown that a gold thin film of only 0.5 nm introduced at the interface between the indium tin oxide or ZnO anode and the organic electron donor in organic photovoltaic cells induces a strong improvement of the cell efficiency. Of course a thickness of 0.5 nm corresponds only to an averaged thickness, the films being too thin to be continuous. For a clear understanding of the physical mechanisms that are responsible for this improved behaviour, it is important to know the fractional coverage and the island height of this thin Au film. In the present work, we have used two different techniques, such as treated scanning electron microscope images and analysis of the inelastic part of peaks of X-ray photoelectron spectroscopy spectra, to estimate the gold coverage and island height of the transparent conductive anode. There is an excellent agreement between the results achieved by both methods. Only 15% of the anode is covered, which proves the high efficiency of gold as an anode buffer layer in organic photovoltaic devices.

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Common Tasks in Microscopic and Ultrastructural Image Analysis Using ImageJ

Cited by 201 publications

References 10 publications

Apoptotic Mediators are Upregulated in the Skeletal Muscle of Chronic/Progressive Mouse Model of Parkinson's Disease

Apoptotic Mediators are Upregulated in the Skeletal Muscle of Chronic/Progressive Mouse Model of Parkinson's Disease

Physiology and behaviour of marine Thioploca

On the ultrathin gold film used as buffer layer at the transparent conductive anode/organic electron donor interface

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