Common laboratory research methods for detection and quantification of cholesterol

Bukiya, Anna N.; Li, Hanxuan; Mysiewicz, Steven; Li, Wei

doi:10.1016/b978-0-323-85857-1.00005-5

Cited by 2 publications

(2 citation statements)

References 160 publications

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“…The Sphygmomanometer will be used to measure both SBP and DBP after 15 to 30 minutes of immobile sitting [8]. The biochemical data of all research participants were collected, including fasting blood glucose, total cholesterol, LDL, VLDL, triglyceride, and HDL cholesterol levels [9][10][11]. They were instructed to report for the biochemical tests after an overnight fast of 8 to 10 hours.…”

Section: Methodsmentioning

confidence: 99%

Anthropological and Biochemical Metabolic Factors in the Normoglycemic, Pre-Diabetic, and Diabetic Metabolic Population

Sorout,

Kacker,

Saboo

et al. 2023

EUMJ

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Background: The term "metabolic syndrome" (MetS) describes a collection of risk factors with metabolic origins that increase the chance of developing type 2 diabetes and cardiovascular disease (CVD). Diabetes is becoming more prevalent in underdeveloped countries, which challenges already limited health expenditures. Aim and Objective: To determine the burden of anthropometric and biochemical metabolic risk factors in normoglycemic, pre diabetics and diabetics metabolic population. Material and Methods: Study conducted in Physiology Department at RUHS College of Medical Sciences and associated hospital, Jaipur, after receiving the Ethical Clearance. The study's sample size was 300. Parameters recorded for data collection: anthropometric (Body Mass Index, Waist Hip Ratio), Blood pressure, biochemical (Fasting blood glucose, HbA1C, Lipid profile parameters). Result: 300 metabolic syndrome subjects were divided into three groups based on their Fasting Blood Glucose level and HbA1C level according to the American Diabetes Association for Diabetes Classification as normal [79 (26.33%)], pre-diabetic [85 (28.33%)], and diabetic [136 (45.33%)].There was significant difference of mean values of age, Waist Hip Ratio, Systolic Blood Pressure, Diastolic Blood Pressure, Fasting Blood Glucose, HbA1C, total cholesterol, High Density Lipoprotein, Low Density Lipoprotein and Triglyceride in three groups. Conclusion: According to the findings of the current study, the metabolic population of pre-diabetics and diabetics has a greater prevalence of metabolic risk factors. The metabolic risk factors grows when fasting blood glucose and HbA1C levels rise. It can also be said that these risk factors contribute to the onset of diabetes, which leads to metabolic syndrome.

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Section: Methodsmentioning

confidence: 99%

Anthropological and Biochemical Metabolic Factors in the Normoglycemic, Pre-Diabetic, and Diabetic Metabolic Population

Sorout,

Kacker,

Saboo

et al. 2023

EUMJ

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show abstract

“…The concentration of the oxidized colorimetric reagent is then measured by photometry ( λ = 500 nm), and the concentration of colored species is directly proportional to the cholesterol content. 4 Although commercial kits for cholesterol determination are easily available, the relatively high number of analytical steps and reagents involved in the process makes it a tedious and time-consuming procedure.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode

et al. 2023

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Common laboratory research methods for detection and quantification of cholesterol

Cited by 2 publications

References 160 publications

Anthropological and Biochemical Metabolic Factors in the Normoglycemic, Pre-Diabetic, and Diabetic Metabolic Population

Anthropological and Biochemical Metabolic Factors in the Normoglycemic, Pre-Diabetic, and Diabetic Metabolic Population

An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode

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