An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode

Abstract

Cholesterol is an important steroid and hormone precursor, and its level in blood is associated with risk factors for cardiovascular diseases. In this work, a non-enzymatic methodology for cholesterol determination...

Effects of derivatization coupled with GC-FID analysis of cholesterol in some bakery products

“…There are also a group of researchers who successfully quantified cholesterol without derivatization from wide range of samples which includes eggs, milk, vegetable oil, human serum and Korean dishes [ [9] , [10] , [11] , [13] , [18] , [19] ]. Among them Choong et al reported high level of cholesterol (193.4–3166.1 μg/g) in vegetable oil which may be the result of overestimation [ 19 ].…”

“…Various methods have been employed to analyze cholesterol, including gravimetry, colorimetry, fluorimetry, and chromatography [ 2 , [7] , [8] , [9] ]. Among these, gas chromatography is considered the most suitable and reliable method to analyzecholesterol, as it allows for the separation of cholesterol from other similar phytosterol compounds [ 10 ].…”

“…Among these, gas chromatography is considered the most suitable and reliable method to analyzecholesterol, as it allows for the separation of cholesterol from other similar phytosterol compounds [ 10 ]. Researchers used both derivatization and non-derivatization technique in gas chromatographic analysis of cholesterol and cholesterol oxidation products [ [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] ]. Several researchers have successfully developed and validated cholesterol analysis methods without derivatization [ [9] , [10] , [11] ].…”

“…Researchers used both derivatization and non-derivatization technique in gas chromatographic analysis of cholesterol and cholesterol oxidation products [ [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] ]. Several researchers have successfully developed and validated cholesterol analysis methods without derivatization [ [9] , [10] , [11] ]. But still now there is no such study that reveals the effect of derivatizations on the cholesterol content and analysisover non-derivatization.…”