Commission Internationale De Botanique Apicole De L'U.I.S.B. : Les Méthodes De La Mélisso-Palynologie

Multi-Element Composition of Honey as a Suitable Tool for Its Authenticity Analysis

“…The pollen analysis was made according to the method of Louveaux et al [1970], using a non-acetolytic method. Ten grams of honey were mixed with about 40 mL of distilled water; then centrifuged at 4500 rpm (3383×g) for 15 min, the supernatant being carefully removed.…”

Palynological evaluation of selected honeys from Romania

“…Both the quantitative and qualitative microscopical analyses were performed according to the method of Louveaux et al (1970), using a non-acetolytic method with some adopted modifications concerning the time and the speed of centrifugation.…”

“…To help combat fraudulent labelling and to identify the geographical and botanical origin of honey, a method of melissopalynology was developed and proposed by the International Commission for Bee Botany (ICBB) (Louveaux et al, 1970). The pollen spectrum of a honey depends on the floral, agricultural and forest conditions where it is produced.…”

“…Examples of plants that are overrepresented include Castanea sativa, Eucalyptus, Brassica napus, while with some other species, the situation is reversed and they are underrepresented in the pollen spectrum. Species in this category include Robinia pseudoacacia, Tilia, Citrus or Rosmarinus (Louveaux et al, 1970;Ricciardelli d'Albore & Vorwohl, 1979;Pérez-Arquillué et al, 1994;Persano-Oddo et al, 1995;von der Ohe et al, 2004).…”

The Pollen Spectrum of the Honey of Tetragonisca Angustula Angustula Latreille (Apidae, Meliponinae)

“…The earliest microscopic studies of honey were made by POSTER (1895); these suggested the possibility of determining the geographical origin of the honey from the pollen contained therein (LouvEAUx, 1968;L OUVEAUX et al, 1970;M AURIZIO , 1975). It was, however, from 1930 onwards that the interest in microscopic investigation of honey increased (MauRtzlo, 1975).…”