Commercialisation of a recombinant vaccine against<i>Boophilus microplus</i>

Willadsen, Peter; Bird, Peter H.; Cobon, Gary S.; Hungerford, J.

doi:10.1017/s0031182000001487

Cited by 298 publications

(187 citation statements)

References 28 publications

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“…Therefore, the development of alternative control strategies is necessary, in which vaccination against ticks is a desirable method. A concealed antigen based-vaccine against Rhiphicephalus (Boophilus) microplus [7] infestation was successfully commercialized [28]. A concealed antigen in the tick gut, however, is unlikely for the boost of the immune response by natural tick exposure compared with salivary glands antigens.…”

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confidence: 99%

Identification of Genes Encoding Cement-Like Antigens Expressed in the Salivary Glands of Haemaphysalis longicornis

Harnnoi

Sakaguchi

Xuan

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. A cDNA expression library from the salivary glands of hard tick, Haemaphysalis longicornis, was constructed. Immunoscreening was performed using sera of the rabbit repeatedly infested with ticks and seventeen positive clones were obtained. A BLASTP search suggested that 8 sequences matched with that of hypothetical H. longicornis sequence and one clone encoded HL35 antigen U from the same tick species. Eight of 17 gave no match to any sequence reported in the database. The proteins expected from these novel sequences possess common characteristics with cement proteins which assist ticks in their attachment to the host during blood feeding. The expression of these genes in salivary glands was confirmed by RT-PCR. Four of the 8 sequences showed to be upregulated upon blood feeding. These immunodominant antigens are of particular interest as candidates for future cement protein based-tick vaccine.

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Identification of Genes Encoding Cement-Like Antigens Expressed in the Salivary Glands of Haemaphysalis longicornis

Harnnoi

Sakaguchi

Xuan

et al. 2006

The Journal of Veterinary Medical Science

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“…A primeira vacina comercial contra carrapatos foi lançada em 1994,na Austrália, tendo o nome de *TickGARD ® , formulada com o antígeno Bm86 (Willadsen et al, 1995). Posteriormente, um grupo cubano clonou essa proteína em levedura Pichia pastoris, sendo esta a segunda vacina formulada, SILVEIRA, W.H., CARVALHO, G.D. e PECONICK, A.P.…”

Section: Controle Imunológicounclassified

Medidas de controle do carrapato Rhipicephalus microplus: uma breve revisão

Silveira¹,

Carvalho²,

Peconick³

2015

Pubvet

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desse carrapato e as diferentes medidas aplicadas no seu controle. A revisão SILVEIRA, W.H., CARVALHO, G.D. e PECONICK, A.P. Medidas de controle do carrapato Rhipicephalus microplus: uma breve revisão. PUBVET, Londrina, V. 8, N. 10, Ed. 259, Art. 1715, Maio, 2014 de literatura embasou-se na consulta bibliográfica a periódicos científicos e livros da área de Medicina Veterinária e Zootecnia que abordavam o tema controle de carrapatos. Por meio das informações obtidas, pode-se observar que o controle químico se faz soberano, porém os métodos de controle alternativos, onde nesse contexto foram abordados, métodos orgânicos, biológicos e imunológicos, além de práticas de manejo de pastagem, tem se demonstrado efeitos satisfatórios no controle do carrapato R. (B.) microplus.Palavras-chave: bovinos, carrapato, Rhipicephalus (Boophilus) microplus. INTRODUÇÃOOs carrapatos são animais originários da Ásia, mais precisamente da Índia e Ilha de Java. Sua expansão mundial se deu por meio das expedições exploradoras, com o transporte de animais e de mercadorias (GONZALES, 1993; MATTHYSSE, 1984) A espécie de carrapatos mais comum em bovinos no Brasil é Boophilus O carrapato dos bovinos encontra-se amplamente difundido no Brasil, o qual possui o maior rebanho bovino comercial do mundo, estimado em 200 milhões de animais, ele é o principal vetor de patologias de alta morbidade e mortalidade como a anaplasmose e a babesiose bovina. Tais doenças também estão associados aos prejuízos determinados pela infestação por carrapatos, os SILVEIRA, W.H., CARVALHO, G.D. e PECONICK, A.P. Medidas de controle do carrapato Rhipicephalus microplus: uma breve revisão. PUBVET, Londrina, V. 8, N. 10, Ed. 259, Art. 1715, Maio, 2014 quais causam anemia, queda na produção e até mesmo a morte de bovinos (BRITO, 2009 Controle QuímicoPor mais de um século, o controle químico pelo uso de acaricidas tem sido a principal forma de controle dos carrapatos, no entanto o uso indiscriminado dos carrapaticidas tem determinado um grave quadro de resistência, de ordem genética, dos carrapatos em relação as drogas (FURLONG, 1993;SANGSTER, 2001;TAYLOR, 2001). A resistência tem se SILVEIRA, W.H., CARVALHO, G.D. e PECONICK, A.P. Medidas de controle do carrapato Rhipicephalus microplus: uma breve revisão. PUBVET, Londrina, V. 8, N. 10, Ed. 259, Art. 1715, Maio, 2014 desenvolvido cada vez mais rápido, de tal forma que a vida útil dos produtos foi reduzida para quatro a cinco anos em média (HONER e GOMES, 1990 Controle OrgânicoA crescente importância da Agricultura Orgânica no mercado nacional e internacional, gera demanda por trabalhos de experimentação científica que testem a eficiência comparativa de diferentes sistemas e técnicas alternativos ao modelo convencional agroquímico e portanto, aceitáveis no contexto das normas orgânicas de certificação (IFOAM, 1992; FAO/OMS, 1999; Min. da Agricultura, 1999;IBD, 1999) (TOLEDO e SOUZA, 2002.No campo da Pecuária Orgânica, uma das prioridades consiste na busca de métodos de controle fitoterápico e homeopático de par...

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“…Targeting proteins expressed on the gut surface has been a particularly successful approach for vaccine development in blood-feeding nematodes (Knox et al, 2003) and ectoparasites such as ticks (Willadsen et al, 1995). Munn (1977) first described contortin, using electron microscopy, as a helical polymeric extracellular structure present in large amounts in the intestine and the pharynx from the L4 and adult stage of the sheep parasite Haemonchus contortus.…”

Section: Introductionmentioning

confidence: 99%

The intestinal contortin structure in Haemonchus contortus: An immobilised anticoagulant?

Geldhof

Knox

2008

International Journal for Parasitology

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Contortin was the first intestinal antigen of the sheep parasite Haemonchus contortus which induced significant levels of protection when used to vaccinate lambs. This antigen is present in the intestine of L4 and adult worms as a helical polymeric structure attached to the luminal surface of the intestinal cells. However, the nature of the protein itself and its function have never been reported. In the present study, contortin was isolated and analysed by peptide mass fingerprint and LC/MS-MS. These analyses indicated that contortin comprises two major proteins, Hc-PCP1 and Hc-PCP2, with homology to prolyl-carboxypeptidases. The two proteins show 64% amino acid sequence identity to each other and both are comprised of two prolyl-carboxypeptidase S28 type domains organised in a tandem repeat. The transcripts of both genes are present from the L4 stage onwards, coinciding with the onset of blood-feeding. Addition of contortin to a fibrinogen solution significantly inhibited blood coagulation in a dose-dependent manner. Mass-spectrometry indicated that the contortin-enriched fraction degraded the C-terminal end of the fibrinogen alpha-chain, which was shown previously to be essential for clot formation. The process happens within seconds after addition and can be inhibited by the dipeptidyl-peptidase IV inhibitors Diprotin A and Bt-PEG-Glu-Pro P (OPh) 2 . These data suggest that the prolyl-carboxypeptidases are intestinal anticoagulants used by H. contortus to interfere with blood coagulation. Ó

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Commercialisation of a recombinant vaccine againstBoophilus microplus

Cited by 298 publications

References 28 publications

Identification of Genes Encoding Cement-Like Antigens Expressed in the Salivary Glands of Haemaphysalis longicornis

Identification of Genes Encoding Cement-Like Antigens Expressed in the Salivary Glands of Haemaphysalis longicornis

Medidas de controle do carrapato Rhipicephalus microplus: uma breve revisão

The intestinal contortin structure in Haemonchus contortus: An immobilised anticoagulant?

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