“…Many transport factors might induce stress in animals, such as distance and duration, microclimatic conditions, trailer design (Schwartzkopf-Genswein et al, 2012) and stocking density (Warriss et al, 1998;Pilcher et al, 2011), as well as the location inside the trailer (Barton Gade et al, 1996;, mixing of unfamiliar animals at loading (De jong et al, 2000;Barton Gade, 2008a and2008b), microclimatic conditions inside the trailer (Driessen, et al, 2020) loading/unloading systems (Faucitano & Goumon, 2018), trailer vibration (Stephens et al, 1985;Gebresenbet et al, 2011;Alambarrio et al, 2022).…”