Commercial Scale Micropropagation of Sugarcane: Constraints and Remedies

Lal, Madan; Tiwari, Ajay; Gupta, G. N.; Kavita, .

doi:10.1007/s12355-014-0345-y

Cited by 31 publications

(19 citation statements)

References 32 publications

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“…Axillary shoot development has been considered as the most trusted and preferred method of micropropagation for the maintenance of clonal fidelity (Lal et al, 2014).…”

Section: Micropropagation For Rapid Seed Cane Multiplicationmentioning

confidence: 99%

“…Contamination of cultures microbes is a severe problem that not only reduces the frequency of shoot culture initiation from the source explants but also the total number of shoots produced at various cycles due to loss of cultures (Lal et al, 2014).…”

Section: Micropropagation For Rapid Seed Cane Multiplicationmentioning

confidence: 99%

“…Use of somaclonal variation that results from either in vitro culture or mutagenic treatments is one of the ways of diversifying the genetic pool and potentially introducing desirable traits (Snyman et al, 2011). The term "somaclonal variation" is used to describe any kind of genetic or epigenetic variation detected in plants derived from cell cultures, irrespective of the morphogenic route or explant used (Lal et al, 2014;Larkin and Scowcroft, 1981). However, other names such as protoclonal, gametoclonal and mericlonal variation are often used to describe variants from protoplasts, anthers and meristem culture, respectively (Bairu et al, 2010).…”

Section: Somaclonal Variation For Sugarcane Improvementmentioning

confidence: 99%

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Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Wekesa¹,

Onguso²,

Nyende³

et al. 2015

Afr. J. Biotechnol.

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Sugarcane (Saccharum officinarum L.) is one of the most important crops in Kenya and has wide range of economic importance. The sugar industry contributes up to 15% to the country's agricultural gross domestic product and an estimated 25% of the population depends on the industry for their livelihood. However, the industry has been facing several challenges including declining yields due to use of poor quality planting materials. There is an increasing pressure to enhance the productivity of sugarcane in order to sustain profitable sugar industries in Kenya, while there are several diseases attacking sugarcane and reducing its quality. Seed multiplication of newly released varieties of sugarcane is one of the major constraints in Kenya as it takes 6-7 years to produce sufficient quantity of improved seed material. In vitro culture offers a practical and fast method for mass propagation of disease-free clonal materials. Successful protocols for shoot tip culture, callus culture, embryo culture, virus free plant production and somatic embryogenesis have already been established. Thus, in vitro technology can be used to enhance productivity of sugarcane in Kenya. Despite several advantages of applying micro-propagation technique in sugarcane such as quick multiplication of newly released varieties, rejuvenation of old deteriorated varieties; production of disease free seed; easy transportation of seed material; elimination of viruses; high cane productivity and sugar yield etc., this technique is not gaining popularity up to the desired extent. There are several constraints like the high cost of production and appearance of some variants in micropropagated population among others. The present article describes the status, challenges and opportunities of in vitro technology for the sugar industry in Kenya. Though, some problems have now been resolved to considerable extents which have been described in this review however, some constraints still require intensive research work to be resolved so that a safe and efficient exploitation of this technique can be ensured in sugarcane seed production programmes for enhanced yields and quality.

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“…Axillary shoot development has been considered as the most trusted and preferred method of micropropagation for the maintenance of clonal fidelity (Lal et al, 2014).…”

Section: Micropropagation For Rapid Seed Cane Multiplicationmentioning

confidence: 99%

Section: Micropropagation For Rapid Seed Cane Multiplicationmentioning

confidence: 99%

Section: Somaclonal Variation For Sugarcane Improvementmentioning

confidence: 99%

See 1 more Smart Citation

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Wekesa¹,

Onguso²,

Nyende³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Despite the importance of sugarcane cultivation, its production is generally characterized by low yield in the field due to, among other causes, virtually zero renewal of plantations because of the lack of vegetative materials certified free of pests and diseases. According to Flores [2] and Lal et al [3], sugarcane varieties age over the years, losing their productive power, which can deteriorate and eventually disappear from the commercial crop. In many countries, most vegetative seed is propagated by conventional methods by sowing bud-containing cuttings.…”

Section: Introductionmentioning

confidence: 99%

“…Sugarcane micropropagation has allowed the rapid multiplication of new varieties, rejuvenation of old deteriorated varieties and sanitation of diseased varieties [3] and has also facilitated the exchange of in vitro plant material. Currently, semiautomation of micropropagation by temporary immersion systems (TISs) offers a practical strategy to reduce production costs [5].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Propagation of Sugarcane for Certified Seed Production

Bello-Bello¹,

Mendoza-Mexicano²,

Pérez-Sato³

2018

Sugarcane - Technology and Research

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Micropropagation of sugarcane is important to obtain pathogen-free plants, genetically homogeneous and invigorate. The micropropagation procedure is divided into stages for the sake of better understanding. Micropropagation for large-scale sugarcane production using a temporary immersion system (TIS) is described. In addition, the aim of this chapter is to report, from the laboratory to the field, the best way to establish and use basic seed (primary seed), semicommercial seed (foundation or secondary seed) and commercial seed production. In conclusion, commercial sugarcane micropropagation enables the massive multiplication of plants to obtain certified vitroplants and increase the sugarcane and sugar productivity per unit area.

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Cell and Tissue Culture Approaches in Relation to Crop Improvement

Gosal

Wani

2018

Biotechnologies of Crop Improvement, Volume 1

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Commercial Scale Micropropagation of Sugarcane: Constraints and Remedies

Cited by 31 publications

References 32 publications

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

In Vitro Propagation of Sugarcane for Certified Seed Production

Cell and Tissue Culture Approaches in Relation to Crop Improvement

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