Commercial Methods for Identification and Susceptibility Testing of Fungi

Moser, Stephen A.; Wicker, Jason A.

doi:10.1002/9781119021872.ch13

Cited by 4 publications

(3 citation statements)

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“…Rapid tests identification of opportunistic fungal pathogens in immunocompromised children and those with HIV or solid organ transplants: Quick TAT as compaed with culture 22Direct testing of CSF (latex agglutination test on a card), enzyme immunoassays for serum/CSF or point-of-care test (POCT) strip test (lateral flow assay akin to pregnancy strip tests) for cryptococcal antigen.Rapid identification of positive fungal growth from sputum, bronchioalveolar lavage, CSF and body fluids/pus/tissues by automated systems (molecular PCR for 18 s/5.8 s rDNA subunit or internal transcribed spacer of the fungus)—this can be targeted directly on normally sterile specimens, but methods are not validated or standardised completely yet.Protein-based identification by MALDI-ToF/MS or rapid conventional biochemical methods.Molecular testing (Accuprobe R ) for dimorphic fungi—has been Food and Drug Administration approved for use in the USA.Multiplex PCR technology (Luminex R ) for most common clinically relevant fungal pathogens (7-plex or 11-plex panels) is currently being explored/evaluated for rapid TAT and simultaneous detection of mixed infections. …”

Section: Introductionmentioning

confidence: 99%

New technologies in microbiology and their potential impact on paediatric practice

Narayanan

2018

Arch Dis Child

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Section: Introductionmentioning

confidence: 99%

New technologies in microbiology and their potential impact on paediatric practice

Narayanan

2018

Arch Dis Child

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“…Recommended techniques for antifungal susceptibility testing of molds are the reference techniques of CLSI and EUCAST. The gradient concentration strip method has shown good correlation with the reference techniques for yeasts and filamentous fungi (28). Nevertheless, only a few studies evaluated the correlation between gradient concentration strips and CLSI (14,(16)(17)(18)(19)(20)(21)(22) or EUCAST (13,15) methodology for Mucorales species.…”

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confidence: 99%

Evaluation of the Gradient Concentration Strip Method for Antifungal Susceptibility Testing of Isavuconazole and Comparators for Mucorales Species

Vidal

Schwarz

Dannaoui

2019

Antimicrob Agents Chemother

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MIC values for amphotericin B and three azoles determined by the EUCAST reference technique and by gradient concentration strips were compared for 30 Mucorales isolates belonging to clinically important species. Essential agreement (EA) within ±2 dilution steps at 24 hours between the techniques was 83.3% for isavuconazole. EAs for itraconazole, amphotericin B, and posaconazole were 86.7%, 73.3%, and 56.7%, respectively. A good agreement was obtained between visual and spectrophotometric readings for EUCAST.

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“…Recently, several clinical evaluations to detect Aspergillus DNA, either in respiratory or in blood-based samples, have clearly shown the diagnostic value of this biomarker (4)(5)(6)(7)(8)(9). In addition, methodological recommendations have been established for PCR protocols (10), and different standardized Aspergillus quantitative PCR (qPCR) kits have been commercialized (11,12). These recent advances show that PCR is now mature for routine use in clinical settings.…”

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Molecular Diagnosis of Invasive Aspergillosis and Detection of Azole Resistance by a Newly Commercialized PCR Kit

et al. 2017

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is the main species responsible for aspergillosis in humans. The diagnosis of aspergillosis remains difficult, and the rapid emergence of azole resistance in is worrisome. The aim of this study was to validate the new MycoGENIE real-time PCR kit and to evaluate its performance on clinical samples for the detection of and its azole resistance. This multiplex assay detects DNA from the species complex by targeting the multicopy 28S rRNA gene and specific TR and L98H mutations in the single-copy-number gene of The specificity of mutation detection was assessed by testing DNA samples from 25 wild-type or mutated clinical isolates. Clinical validation was performed on 88 respiratory samples obtained from 62 patients and on 69 serum samples obtained from 16 patients with proven or probable aspergillosis and 13 patients without aspergillosis. The limit of detection was <1 copy for the 28S rRNA gene and 6 copies for the gene harboring the TR and L98H alterations. No cross-reactivity was detected with various fungi and bacteria. All isolates harboring the TR and L98H mutations were accurately detected by quantitative PCR (qPCR) analysis. With respiratory samples, qPCR results showed a sensitivity and specificity of 92.9% and 90.1%, respectively, while with serum samples, the sensitivity and specificity were 100% and 84.6%, respectively. Our study demonstrated that this new real-time PCR kit enables sensitive and rapid detection of DNA and azole resistance due to TR and L98H mutations in clinical samples.

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Commercial Methods for Identification and Susceptibility Testing of Fungi

Cited by 4 publications

References 304 publications

New technologies in microbiology and their potential impact on paediatric practice

New technologies in microbiology and their potential impact on paediatric practice

Evaluation of the Gradient Concentration Strip Method for Antifungal Susceptibility Testing of Isavuconazole and Comparators for Mucorales Species

Molecular Diagnosis of Invasive Aspergillosis and Detection of Azole Resistance by a Newly Commercialized PCR Kit

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