New technologies in microbiology and their potential impact on paediatric practice

Narayanan, Manjusha

doi:10.1136/archdischild-2017-314189

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…However, this approach suffers from a relatively poor limit of detection (LOD) of 10 5 -10 6 colony-forming units (CFU). 4 Nucleic acid sequencing also requires expensive instruments and trained personnel, and this method can therefore exceed the budgetary constraints of small clinical laboratories. 3,5 Various biosensors have been implemented to make rapid and sensitive detection of S. aureus or other pathogenic bacteria, such as surface plasmon resonance (SPR), 6 surface-enhanced Raman scattering (SERS), 7 colorimetry, 8 electrochemistry 9 and terahertz (THz) spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive detection ofStaphylococcus aureusby a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification

Yang

Huang

et al. 2020

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Highly sensitive detection ofStaphylococcus aureusby a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification

Yang

Huang

et al. 2020

RSC Adv.

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The management of anti-infective agents in intensive care units: the potential role of a ‘fast’ pharmacology

Cattaneo

Corona

Rosa

et al. 2020

Expert Review of Clinical Pharmacology

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Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

Schulze

Wilson

Cara

et al. 2021

Sensors

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Rapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.

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New technologies in microbiology and their potential impact on paediatric practice

Cited by 3 publications

References 34 publications

Highly sensitive detection ofStaphylococcus aureusby a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification

Highly sensitive detection ofStaphylococcus aureusby a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification

The management of anti-infective agents in intensive care units: the potential role of a ‘fast’ pharmacology

Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

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