Commentary: Systems Biology Approach to Model the Life Cycle of Trypanosoma cruzi

Carrea, Alejandra; Diambra, Luis

doi:10.3389/fcimb.2017.00001

Cited by 87 publications

(137 citation statements)

References 28 publications

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“…It was previously shown through in silico studies that DMTU is a peptidase domain inhibitor of the ABC transporter protein (ComA) in S. mutans [ 33 , 34 ]. This study showed the targeted activity indirectly, by considering only the virulence genes downstream of the ComA protein and that the comA gene was not downregulated.…”

Section: Resultsmentioning

confidence: 99%

“…DMTU (1,3-di-m-tolyl-urea) is a biocompatible, aromatic compound which has been reported to demonstrate antibiofilm effects against the cariogenic bacterium, Streptococcus mutans , by inhibiting its quorum sensing pathway (comDE). Notably, it has immunomodulatory and anti-infective properties in Wistar rats infected with S. mutans [ 33 , 34 ]. Other urea derivatives, such as (S-3, 4-dicholoro benzene)-isothiourea hydrochloride, have been shown to competitively binds to mreB, a cell wall protein that is widely present in Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Small Molecule, 1,3-di-m-tolyl-urea, Inhibits and Disrupts Multispecies Oral Biofilms

et al. 2020

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An imbalance of homeostasis between the microbial communities and the host system leads to dysbiosis in oral micro flora. DMTU (1,3-di-m-tolyl-urea) is a biocompatible compound that was shown to inhibit Streptococcus mutans biofilm by inhibiting its communication system (quorum sensing). Here, we hypothesized that DMTU is able to inhibit multispecies biofilms. We developed a multispecies oral biofilm model, comprising an early colonizer Streptococcus gordonii, a bridge colonizer Fusobacterium nucleatum, and late colonizers Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. We performed comprehensive investigations to demonstrate the effect of DMTU on planktonic cells and biofilms. Our findings showed that DMTU inhibits and disrupts multispecies biofilms without bactericidal effects. Mechanistic studies revealed a significant down regulation of biofilm and virulence-related genes in P. gingivalis. Taken together, our study highlights the potential of DMTU to inhibit polymicrobial biofilm communities and their virulence.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Small Molecule, 1,3-di-m-tolyl-urea, Inhibits and Disrupts Multispecies Oral Biofilms

et al. 2020

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“…Could the chronic inflammation triggered by mucosal leishmaniasis induce the development of lymphoma as can occur in the case of Helicobacter pylori infection and the development of mucosa-associated lymphatic tissue (MALT) lymphoma of the gastrointestinal tract [ 9 , 10 , 11 ]? We believe that the treatment of nasal leishmaniasis may be sufficient to improve local lymphoproliferation and to heal the lesion.…”

Section: Resultsmentioning

confidence: 99%

Mucosal leishmaniasis mimicking T-cell lymphoma in a patient receiving monoclonal antibody against TNFα

et al. 2017

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“…Also two genes that coding for glycolitic enzyme enolases were indicated as externally regulated during transition Tzd2 → Bz21 by our analysis (clusters 364 and 376). It is important to note that enolases are recognized moonlight proteins, i.e., proteins that have dual functions 22,23 ; in T. gondii enolases fulfill a second function as transcriptional regulators implicated in parasite differentiation and cyst formation 24,25 .…”

Section: Modeling the Phenotypic Transitions Of T Gondiimentioning

confidence: 99%

Gene target discovery with network analysis inToxoplasma gondii

Alonso

Corvi

Diambra

2018

Preprint

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Infectious diseases are of great relevance for global health, but needed drugs and vaccines have not been developed yet or are not effective in many cases. In fact, traditional scientific approaches with intense focus on individual genes or proteins have not been successful in providing new treatments. Hence, innovations in technology and computational methods provide new tools to further understand complex biological systems such as pathogens biology. In this work, we propose a system biology approach to analyze transcriptomic data of the parasite Toxoplasma gondii. By means of an optimization procedure, the phenotypic transitions between the stages associated with the life cycle of the parasite were embedded into the dynamics of a gene network model. Thus, through this methodology we were able to reconstruct a gene regulatory network able to emulate the life cycle of the pathogen. The community network analysis has revealed that nodes of the network can be organized in seven communities which allow us to assign putative functions to 339 previously uncharacterized genes, 25 of which are predicted as new pathogenic factors. Furthermore, we identified a small subnetwork module that controls the parasite's life cycle. These new findings can contribute to understand of parasite pathogenesis.

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Commentary: Systems Biology Approach to Model the Life Cycle of Trypanosoma cruzi

Cited by 87 publications

References 28 publications

A Novel Small Molecule, 1,3-di-m-tolyl-urea, Inhibits and Disrupts Multispecies Oral Biofilms

A Novel Small Molecule, 1,3-di-m-tolyl-urea, Inhibits and Disrupts Multispecies Oral Biofilms

Mucosal leishmaniasis mimicking T-cell lymphoma in a patient receiving monoclonal antibody against TNFα

Gene target discovery with network analysis inToxoplasma gondii

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