A Novel Small Molecule, 1,3-di-m-tolyl-urea, Inhibits and Disrupts Multispecies Oral Biofilms

Kalimuthu, Shanthini; Cheung, Bmy; Yau, J. Y. Y.; Shanmugam, Karthi; Solomon, Adline Princy; Neelakantan, Prasanna

doi:10.3390/microorganisms8091261

Cited by 8 publications

(6 citation statements)

References 59 publications

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“…It has proved inhibitory effects against S. mutans biofilm formation as well as multispecies biofilm models. For example, Kalimuthu et al have used Streptococcus gordonii as an early colonizer, Fusobacterium nucleatum as a bridge colonizer, and Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans as late colonizers to develop a multispecies oral biofilm model and demonstrates the anti-microflora effect of DMTU against the planktonic cells in the multispecies biofilm model [ 60 ]. ZY354 is another synthetic small molecule with low toxicity against human oral cell lines and acceptable bactericidal function against common oral streptococci (i.e., S. mutans , S. gordonii , and S. sanguinis ).…”

Section: Chemical Antibiofilm Materialsmentioning

confidence: 99%

The Current Antimicrobial and Antibiofilm Activities of Synthetic/Herbal/Biomaterials in Dental Application

Moghaddam

Ranjbar

Yazdanian

et al. 2022

BioMed Research International

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Herbal and chemical products are used for oral care and biofilm treatment and also have been reported to be controversial in the massive trials conducted in this regard. The present review is aimed at evaluating the potential of relevant herbal and chemical products and comparing their outcomes to conventional oral care products and summarizing the current state of evidence of the antibiofilm properties of different products by evaluating studies from the past eleven years. Chlorhexidine gluconate (CHX), essential oils (EOs), and acetylpyridinium chloride were, respectively, the most commonly studied agents in the included studies. As confirmed by all systematic reviews, CHX and EO significantly control the plaque formation and gingival indices. Fluoride is another interesting reagent in oral care products that has shown promising results of oral health improvement, but the evidence quality needs to be refined. The synergy between natural plants and chemical products should be targeted in the future to accede to the formation of new, efficient, and healthy anticaries strategies. Moreover, to discover their biofilm-interfering or biofilm-inhibiting activities, effective clinical trials are needed. In this review article, therapeutic applications of herbal/chemical materials in oral biofilm infections are discussed in recent years (2010-2022).

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Section: Chemical Antibiofilm Materialsmentioning

confidence: 99%

The Current Antimicrobial and Antibiofilm Activities of Synthetic/Herbal/Biomaterials in Dental Application

Moghaddam

Ranjbar

Yazdanian

et al. 2022

BioMed Research International

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“…To assess the biofilm metabolic activity, a reaction solution of PBS, 1 mg/ml of XTT (2,3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide) and menadione (70 μg/ml) was freshly prepared at a ratio of 79:20:1. Two hundred microliters of the reaction solution was added to the wells and incubated in dark conditions for 3 h at 37°C [37]. After incubation, 100 μl of the supernatant was transferred to a new plate and the absorbance was measured at 492 nm.…”

Section: Methodsmentioning

confidence: 99%

“…CC-BY 4.0 International license made available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is The copyright holder for this preprint this version posted March 15, 2021. ; https://doi.org/10.1101/2021.03.15.435450 doi: bioRxiv preprint 6 incubated in dark conditions for 3 h at 37°C [37]. After incubation, 100 µl of the supernatant was transferred to a new plate and the absorbance was measured at 492 nm.…”

Section: Assessment Of Biofilm Metabolic Activitymentioning

confidence: 99%

Trans-cinnamaldehyde attenuatesEnterococcus faecalisvirulence and inhibits biofilm formation

Ali

Matinlinna

Lévesque

et al. 2021

Preprint

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Enterococcus faecalis as an important nosocomial pathogen is critically implicated in the pathogenesis of endocarditis, urinary tract and surgical wound infections. Its major virulence attributes (biofilm formation, production of proteases and hemolytic toxins) enable it to cause extensive host tissue damage. With the alarming increase in enterococcal resistance to antibiotics, novel therapeutics are required to inhibit E. faecalis biofilm formation and virulence. Trans -cinnamaldehyde (TC), the main phytochemical in cinnamon essential oils has demonstrated promising activity against a wide range of pathogens. Here, we comprehensively investigated the effect of TC on planktonic growth, biofilm formation, proteolytic and hemolytic activities, as well as gene regulation in E. faecalis . Our findings revealed that sub-inhibitory concentrations of TC reduced biofilm formation, biofilm exopolysaccharides as well as its proteolytic and hemolytic activities. Mechanistic studies revealed significant down regulation of the quorum sensing fsr locus and downstream gelE , which are major virulence regulators in E. faecalis . Taken together, our study highlights the potential of TC to inhibit E. faecalis biofilm formation and its virulence.

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“…In addition, DMTU significantly inhibited the formation of multispecies biofilms consisting of Streptococcus gordonii , Fusobacterium nucleatum , Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans at 12.5μM without affecting cell viability. DMTU also down-regulated the expression of virulence genes in P. gingivalis such as mfa1, rgpA and rgpB ( Kalimuthu et al., 2020 ).…”

Section: Small Molecules That Inhibit Quorum Sensing Systemmentioning

confidence: 99%

The Application of Small Molecules to the Control of Typical Species Associated With Oral Infectious Diseases

Shen

Lyu

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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Oral microbial dysbiosis is the major causative factor for common oral infectious diseases including dental caries and periodontal diseases. Interventions that can lessen the microbial virulence and reconstitute microbial ecology have drawn increasing attention in the development of novel therapeutics for oral diseases. Antimicrobial small molecules are a series of natural or synthetic bioactive compounds that have shown inhibitory effect on oral microbiota associated with oral infectious diseases. Novel small molecules, which can either selectively inhibit keystone microbes that drive dysbiosis of oral microbiota or inhibit the key virulence of the microbial community without necessarily killing the microbes, are promising for the ecological management of oral diseases. Here we discussed the research progress in the development of antimicrobial small molecules and delivery systems, with a particular focus on their antimicrobial activity against typical species associated with oral infectious diseases and the underlying mechanisms.

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A Novel Small Molecule, 1,3-di-m-tolyl-urea, Inhibits and Disrupts Multispecies Oral Biofilms

Cited by 8 publications

References 59 publications

The Current Antimicrobial and Antibiofilm Activities of Synthetic/Herbal/Biomaterials in Dental Application

The Current Antimicrobial and Antibiofilm Activities of Synthetic/Herbal/Biomaterials in Dental Application

Trans-cinnamaldehyde attenuatesEnterococcus faecalisvirulence and inhibits biofilm formation

The Application of Small Molecules to the Control of Typical Species Associated With Oral Infectious Diseases

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