Cometabolic biodegradation of methyl tert-butyl ether by a soil consortium. Effect of components present in gasoline.

Garnier, P.M.; Auria, Richard; Augur, Christopher; Revah, Sergio

doi:10.2323/jgam.46.79

Cited by 27 publications

(20 citation statements)

References 15 publications

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“…While this is a common scenario, especially among bacteria isolated from contaminated sites with one or multiple of those compounds, many microorganisms are able to co-metabolize the non-growth substrates concerned only when a growth-supporting substrate is supplemented (Garnier et al 2000;Liu et al 2001a). Strain X1 investigated in this study is a good example.…”

Section: Discussionmentioning

confidence: 99%

Co-metabolic degradation of dimethoate by Raoultella sp. X1

et al. 2008

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A bacterium Raoultella sp. X1, based on its 16S rRNA gene sequence, was isolated. Characteristics regarding the bacterial morphology, physiology, and genetics were investigated with an electron microscopy and conventional microbiological techniques. Although the isolate grew and degraded dimethoate poorly when the chemical was used as a sole carbon and energy source, it was able to remove up to 75% of dimethoate via co-metabolism. With a response surface methodology, we optimized carbon, nitrogen and phosphorus concentrations of the media for dimethoate degradation. Raoultella sp. X1 has a potential to be a useful organism for dimethoate degradation and a model strain for studying this biological process at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Co-metabolic degradation of dimethoate by Raoultella sp. X1

et al. 2008

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“…A number of ß-proteobacteria typified by Methylibium petroleiphilum PM1 utilize both MTBE and TBA as sole sources of carbon and energy for growth and TBA is regarded as an obligate MTBE-derived metabolite in these organisms (Hanson et al 1999;Müller et al 2008;Nakatsu et al 2006;Zaitsev et al 2007). Another more diverse group of bacteria, including pseudomonads (Garnier et al 1999(Garnier et al , 2000Smith and Hyman 2004;Smith et al 2003b), mycobacteria (Smith et al 2003a) and other actinomycetes (Liu et al 2001) can cometabolize MTBE after growth on propane (Steffan et al 1997;Smith et al 2003a), longer chain n-alkanes (Garnier et al 1999;Liu et al 2001;Smith et al 2003b, Smith and and isoalkanes (Hyman et al 2000). The MTBE-oxidizing activity of these organisms has often been attributed to the lack of substrate specificity of monooxygenase enzymes otherwise responsible for initiating alkane oxidation (Hardison et al 1997;Steffan et al 1997;Smith et al 2003a;Smith and Hyman 2004).…”

Section: Introductionmentioning

confidence: 98%

Effects of gasoline components on MTBE and TBA cometabolism by Mycobacterium austroafricanum JOB5

House

Hyman

2009

Biodegradation

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In this study we have examined the effects of individual gasoline hydrocarbons (C(5-10,12,14) n-alkanes, C(5-8) isoalkanes, alicyclics [cyclopentane and methylcyclopentane] and BTEX compounds [benzene, toluene, ethylbenzene, m-, o-, and p-xylene]) on cometabolism of methyl tertiary butyl ether (MTBE) and tertiary butyl alcohol (TBA) by Mycobacterium austroafricanum JOB5. All of the alkanes tested supported growth and both MTBE and TBA oxidation. Growth on C(5-8) n-alkanes and isoalkanes was inhibited by acetylene whereas growth on longer chain n-alkanes was largely unaffected by this gas. However, oxidation of both MTBE and TBA by resting cells was consistently inhibited by acetylene, irrespective of the alkane used as growth-supporting substrate. A model involving two separate but co-expressed alkane-oxidizing enzyme systems is proposed to account for these observations. Cyclopentane, methylcyclopentane, benzene and ethylbenzene did not support growth but these compounds all inhibited MTBE and TBA oxidation by alkane-grown cells. In the case of benzene, the inhibition was shown to be due to competitive interactions with both MTBE and TBA. Several aromatic compounds (p-xylene > toluene > m-xylene) did support growth and cells previously grown on these substrates also oxidized MTBE and TBA. Low concentrations of toluene (<10 microM) stimulated MTBE and TBA oxidation by alkane-grown cells whereas higher concentrations were inhibitory. The effects of acetylene suggest strain JOB5 also has two distinct toluene-oxidizing activities. These results have been discussed in terms of their impact on our understanding of MTBE and TBA cometabolism and the enzymes involved in these processes in mycobacteria and other bacteria.

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“…Alkanes, particularly n-alkanes, are important components of crude oils and its derivatives, such as heating oil, jet fuel, gasoline and kerosene (Marin et al 1995; Berekaa and Steinbüchel 2000). In nature, some microorganisms oxidize aerobically (Berekaa and Steinbüchel 2000; Solano-Serena et al2000; Dutta and Harayama 2001) and anaerobically (Chayabutra and Ju 2000; Kniemeyer et al 2003), co-metabolize (Whyte et al 1997; Garnier et al 2000) and detoxify most of the C4-C20 compounds from linear, branched and cyclic alkanes (Scott and Finnerty 1976; Leahy and Colwell 1990), including low-carbon hydrocarbons, which may affect cell membrane integrity (Marin et al 1995). Particularly, alkanes that are metabolized via oxidation are used as a carbon source for cell growth.…”

Section: Introductionmentioning

confidence: 99%

Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925

2011

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Accidental oil spills and waste disposal are important sources for environmental pollution. We investigated the biodegradation of alkanes by Pseudomonas aeruginosa ATCC 55925 in relation to a rhamnolipid surfactant produced by the same bacterial strain. Results showed that the linear C11-C21 compounds in a heating oil sample degraded from 6% to 100%, whereas the iso-alkanes tended to be recalcitrant unless they were exposed to the biosurfactant; under such condition total biodegradation was achieved. Only the biodegradation of the commercial C12-C19 alkanes could be demonstrated, ranging from 23% to 100%, depending on the experimental conditions. Pristane (a C19 branched alkane) only biodegraded when present alone with the biosurfactant and when included in an artificial mixture even without the biosurfactant. In all cases the biosurfactant significantly enhanced biodegradation. The electron scanning microscopy showed that cells depicted several adaptations to growth on hydrocarbons, such as biopolymeric spheres with embedded cells distributed over different layers on the spherical surfaces and cells linked to each other by extracellular appendages. Electron transmission microscopy revealed transparent inclusions, which were associated with hydrocarbon based-culture cells. These patterns of hydrocarbon biodegradation and cell adaptations depended on the substrate bioavailability, type and length of hydrocarbon.

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Cometabolic biodegradation of methyl tert-butyl ether by a soil consortium. Effect of components present in gasoline.

Cited by 27 publications

References 15 publications

Co-metabolic degradation of dimethoate by Raoultella sp. X1

Co-metabolic degradation of dimethoate by Raoultella sp. X1

Effects of gasoline components on MTBE and TBA cometabolism by Mycobacterium austroafricanum JOB5

Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925

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