Combining surface chemistry modification and <i>in situ</i> small-angle scattering characterization to understand and optimize the biological behavior of nanomedicines

Goas, Marine Le; Roussel, Tom; Kalbazova, Maria; Carriere, David; Barruet, Elodie; Geertsen, Valérie; Fadda, G. C.; Testard, Fabienne; Carrot, Géraldine; Renault, Jean-Philippe

doi:10.1039/d0tb01167c

Cited by 4 publications

(11 citation statements)

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“…The library of polymethacrylate-grafted AuNPs was synthesized through a direct method, described in a previous paper . Five methacrylate monomers were used: methacrylic acid (MAA), n -butyl methacrylate ( n BuMA), hydroxylethyl methacrylate (HEMA), dimethylaminoethyl methacrylate (DMAEMA), and poly(ethylene glycol) methacrylate (PEGMA) (see chemical structures in Figure S1).…”

Section: Resultsmentioning

confidence: 99%

“…Detailed characterization of the resulting NPs was performed using various techniques, all of which were described in a previous paper: ζ-potential, TEM, SAXS, and SANS. Briefly, for each type of NP, SAXS and TEM enabled to determine the shape and mean diameter of the gold core, while SANS allowed to obtain the radius of gyration of the whole NP via two different methods (Guinier approximation and star model), as well as the grafting density of polymer ligands.…”

Section: Resultsmentioning

confidence: 99%

“…All characteristics are summarized in Table . Using SAXS, excellent colloidal stability was shown in various biological media for all NPs, whatever the nature of the polymer corona …”

Section: Resultsmentioning

confidence: 99%

“…The synthesis and characterization have already been described elsewhere. , Briefly, a variety of polymethacrylate ligands was obtained through an atom-transfer radical polymerization (ATRP). Gold nanoparticles (AuNPs) were then synthesized directly in the presence of the polymethacrylate ligands, via the reduction of HAuCl 4 .…”

Section: Methodsmentioning

confidence: 99%

“…The chosen hybrid NPs were composed of a gold core covered by grafted polymethacrylate chains of different nature. These nano-objects could be considered as models as they shared the same structural properties but differed by their charge, ligand size, and hydrophobicity/hydrophilicity balance, as it has already been shown in a previous study . Reliable comparison of the impact of their physicochemical properties was therefore possible.…”

Section: Introductionmentioning

confidence: 98%

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How Do Surface Properties of Nanoparticles Influence Their Diffusion in the Extracellular Matrix? A Model Study in Matrigel Using Polymer-Grafted Nanoparticles

et al. 2020

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Diffusion of nanomedicines inside the extracellular matrix (ECM) has been identified as a key factor to achieve homogeneous distribution and therefore therapeutic efficacy. Here, we sought to determine the impact of nanoparticles’ (NPs) surface properties on their ability to diffuse in the ECM. As model nano-objects, we used a library of gold nanoparticles grafted with a versatile polymethacrylate corona, which enabled the surface properties to be modified. To accurately recreate the features of the native ECM, diffusion studies were carried out in a tumor-derived gel (Matrigel). We developed two methods to evaluate the diffusion ability of NPs inside this model gel: an easy-to-implement one based on optical monitoring and another one using small-angle X-ray scattering (SAXS) measurements. Both enabled the determination of the diffusion coefficients of NPs and comparison of the influence of their various surface properties, while the SAXS technique also allowed to monitor the NPs’ structure as they diffused inside the gel. Positive charges and hydrophobicity were found to particularly hinder diffusion, and the different results suggested on the whole the presence of NPs–matrix interactions, therefore underlying the importance of the ECM model. The accuracy of the tumor-derived gels used in this study was evidenced by in vivo experiments involving intratumoral injections of NPs on mice, which showed that diffusion patterns in the peripheral tumor tissues were quite similar to the ones obtained within the chosen ECM model.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…All characteristics are summarized in Table . Using SAXS, excellent colloidal stability was shown in various biological media for all NPs, whatever the nature of the polymer corona …”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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