Combining Normal/Reversed-Phase HPTLC with Univariate Calibration for the Piperine Quantification with Traditional and Ultrasound-Assisted Extracts of Various Food Spices of Piper nigrum L. under Green Analytical Chemistry Viewpoint

Abstract

Due to unavailability of sustainable analytical techniques for the quantitation of piperine (PPN) in food and pharmaceutical samples, there was a need to develop a rapid and sensitive sustainable analytical technique for the quantitation of PPN. Therefore, the current research presents a fast and highly sensitive normal/reversed-phase high-performance thin-layer chromatography (HPTLC) technique with classical univariate calibration for the quantitation of PPN in various food spices of black pepper with traditional (TE) and ultrasound-assisted extracts (UBE) of various food spices of Piper nigrum L. under green analytical chemistry viewpoint. The amount of PPN in TE of four different spices of black pepper—namely BPMH, BPLU, BPSH, and BPPA—was found to be 309.53, 304.97, 282.82, and 232.73 mg g−1, respectively using a sustainable normal-phase HPTLC technique. However, the amount of PPN in UBE of BPMH, BPLU, BPSH, and BPPA was recorded as 318.52, 314.60, 292.41, and 241.82 mg g−1, respectively using a sustainable normal phase HPTLC technique. The greenness of normal/reversed-phase HPTLC technique was predicted using AGREE metric approach. The eco-scale was found to be 0.90, suggested excellent greenness of normal/reversed-phase technique. UBE of PPN was also found to be superior over TE of PPN. Overall, the results of this research suggested that the proposed normal/reversed-phase densitometry technique could be effectively used for the quantitation of PPN in food and pharmaceutical samples.

Investigating Antiarthritic Potential of Nanostructured Clove Oil (Syzygium aromaticum) in FCA-Induced Arthritic Rats: Pharmaceutical Action and Delivery Strategies

“…HPTLC method was employed for all analyses of marker compound as it is a simple and economical method [ 36 ]. The HPTLC approach was used to quantify an active ingredient of CO, namely EU (CAMAG, Muttenz, Switzerland).…”

“…We adopted an aqueous titration method followed by high shear mixing for formulation development. A high-performance thin layer chromatography (HPTLC) method was employed for all analyses of the marker compound as it is a simple and economical method [ 36 ]. Pharmaceutical evaluation, product stability, and biological studies were performed as per previously published reports [ 2 , 32 , 37 ].…”

Validated HPTLC and antioxidant activities for quality control of catechin in a fermented tea (Camellia sinensis var. assamica)

“…Method specificity of HPTLC was normally obtained by comparing retardation factor ( R f ) of standard analyte. R f value of analyte should not be interfered with other substances in the sample matrix (Alam et al., 2020; Alqarni et al., 2021; Foudah et al., 2020). Peak purity was evaluated by comparing the spectrums of standard catechin with CE sample.…”

Determination of Thymol in Commercial Formulation, Essential Oils, Traditional, and Ultrasound-Based Extracts of Thymus vulgaris and Origanum vulgare Using a Greener HPTLC Approach

“…In addition, the greener or environmental-friendly HPTLC approaches are not yet documented for thymol analysis in commercial formulations, essential oils, and plant-based phytopharmaceuticals. Greener HPTLC approaches offer many merits, which include "simplicity, economicity, low operation cost, short analysis time, parallel analysis of multiple samples, detection clarity, and reduction in environmental toxicity" compared to other analytical approaches [40][41][42][43]. Hence, a normal-phase HPTLC approach for thymol analysis was utilized in this study.…”