Combining magnetic nanoparticle capture and poly-enzyme nanobead amplification for ultrasensitive detection and discrimination of DNA single nucleotide polymorphisms

Lapitan, Lorico DS.; Xu, Yihan; Guo, Yuan; Zhou, Dejian

doi:10.1039/c8nr07641c

Cited by 25 publications

(9 citation statements)

References 69 publications

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“…Recently, some researchers tried to develop novel types of biosensor for virus DNA analysis, such as nanoparticle‐assembled photonic crystal arrays, electrochemical assays, dark field sensors using plasmonic nanoparticles, piezoelectric plate sensors and fluorescence assays . To improve the sensitivity, some amplification strategies have been used like polymerase chain reaction (PCR), ligation‐based amplification, rolling circle amplification (RCA), loop mediated isothermal amplification (LAMP), entropy‐driven catalysis, and hybridization chain reaction (HCR) . The employment of signal sources with high signal intensity may also aid the improvement of sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Carbon Nanodot–Based Fluorescent Method for Virus DNA Analysis with Isothermal Strand Displacement Amplification

Yang

Guo

Wang³

et al. 2019

Part & Part Syst Charact

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In this work, a fluorescent method is developed for ultrasensitive detection of virus DNA, coupling DNA‐modified carbon nanodots (CDs) and an isothermal amplification technology. The sensitivity is significantly improved with cyclic strand displacement polymerization and highly luminescent CDs. Taking the hemagglutinin7 (H7) gene and neuraminidase 9 (N9) gene as examples, the limits of detection are 4.6 × 10−15 and 3.4 × 10−15 m, respectively. Furthermore, the proposed method is highly selective and capable of detecting target sequences in biological samples, indicating great potential for applications in life science research and clinical diagnosis.

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Section: Methodsmentioning

confidence: 99%

Carbon Nanodot–Based Fluorescent Method for Virus DNA Analysis with Isothermal Strand Displacement Amplification

Yang

Guo

Wang³

et al. 2019

Part & Part Syst Charact

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“…The whole method improved the sensitivity and detection of cancer genetic mutations such as V-KI-RAS2 Kirsten rat sarcoma viral oncogene homolog ( KRAS ) gene mutations. These somatic mutations are common in lung and colorectal cancers and were used as a model DNA target in the study [ 96 ]. The method used in the research utilized the hybrid approach in which MNPs were combined with poly-enzyme nanobead signal amplification.…”

Section: Detection Of Snpsmentioning

confidence: 99%

Application of Nanotechnology for Sensitive Detection of Low-Abundance Single-Nucleotide Variations in Genomic DNA: A Review

et al. 2021

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Single-nucleotide polymorphisms (SNPs) are the simplest and most common type of DNA variations in the human genome. This class of attractive genetic markers, along with point mutations, have been associated with the risk of developing a wide range of diseases, including cancer, cardiovascular diseases, autoimmune diseases, and neurodegenerative diseases. Several existing methods to detect SNPs and mutations in body fluids have faced limitations. Therefore, there is a need to focus on developing noninvasive future polymerase chain reaction (PCR)–free tools to detect low-abundant SNPs in such specimens. The detection of small concentrations of SNPs in the presence of a large background of wild-type genes is the biggest hurdle. Hence, the screening and detection of SNPs need efficient and straightforward strategies. Suitable amplification methods are being explored to avoid high-throughput settings and laborious efforts. Therefore, currently, DNA sensing methods are being explored for the ultrasensitive detection of SNPs based on the concept of nanotechnology. Owing to their small size and improved surface area, nanomaterials hold the extensive capacity to be used as biosensors in the genotyping and highly sensitive recognition of single-base mismatch in the presence of incomparable wild-type DNA fragments. Different nanomaterials have been combined with imaging and sensing techniques and amplification methods to facilitate the less time-consuming and easy detection of SNPs in different diseases. This review aims to highlight some of the most recent findings on the aspects of nanotechnology-based SNP sensing methods used for the specific and ultrasensitive detection of low-concentration SNPs and rare mutations.

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Section: Ferrofluid‐based Detection Diagnosis and Therapymentioning

confidence: 99%

“…Similarly, DNA or DNA methylation site (e.g., 5‐hydroxymethylcytosine) could also be quantifiably detected with the help of ferromagnetic particles . Recently, a label‐free full‐match DNA detection platform was set up . In this platform, a pair of DNA probes (capture and signal DNA) were employed, and both of their sequences were complementary to a half of the target DNA.…”

Section: Ferrofluid‐based Detection Diagnosis and Therapymentioning

confidence: 99%

Flexible Ferrofluids: Design and Applications

et al. 2019

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Ferrofluids, also known as ferromagnetic particle suspensions, are materials with an excellent magnetic response, which have attracted increasing interest in both industrial production and scientific research areas. Because of their outstanding features, such as rapid magnetic reaction, flexible flowability, as well as tunable optical and thermal properties, ferrofluids have found applications in various fields, including material science, physics, chemistry, biology, medicine, and engineering. Here, a comprehensive, in‐depth insight into the diverse applications of ferrofluids from material fabrication, droplet manipulation, and biomedicine to energy and machinery is provided. Design of ferrofluid‐related devices, recent developments, as well as present challenges and future prospects are also outlined.

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Combining magnetic nanoparticle capture and poly-enzyme nanobead amplification for ultrasensitive detection and discrimination of DNA single nucleotide polymorphisms

Abstract: An ultrasensitive sensor which combines magnetic capture and poly-enzyme nanobead amplification to quantify a low aM DNA target is developed.

Cited by 25 publications

References 69 publications

Carbon Nanodot–Based Fluorescent Method for Virus DNA Analysis with Isothermal Strand Displacement Amplification

Carbon Nanodot–Based Fluorescent Method for Virus DNA Analysis with Isothermal Strand Displacement Amplification

Application of Nanotechnology for Sensitive Detection of Low-Abundance Single-Nucleotide Variations in Genomic DNA: A Review

Flexible Ferrofluids: Design and Applications

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