Carbon Nanodot–Based Fluorescent Method for Virus DNA Analysis with Isothermal Strand Displacement Amplification

Abstract

In this work, a fluorescent method is developed for ultrasensitive detection of virus DNA, coupling DNA‐modified carbon nanodots (CDs) and an isothermal amplification technology. The sensitivity is significantly improved with cyclic strand displacement polymerization and highly luminescent CDs. Taking the hemagglutinin7 (H7) gene and neuraminidase 9 (N9) gene as examples, the limits of detection are 4.6 × 10−15 and 3.4 × 10−15 m, respectively. Furthermore, the proposed method is highly selective and capable of detecting target sequences in biological samples, indicating great potential for applications in life science research and clinical diagnosis.

Carbon Dots: A New Type of Carbon-Based Nanomaterial with Wide Applications

“… 77 In addition, benefiting from the dual-model response to pH changing as well as the established analytical method, wound pH could be both predicted theoretically and estimated visually in the case of blood contamination and long-term observations. Besides applications in ions and pH sensing, CDs also show selectivity toward a wide range of biomolecules including amino acids (such as cysteine), 78 glutathione, 79 vitamins (such as vitamin B 12 , vitamin B 7 , ascorbic acid), 80 − 82 formaldehyde, 83 glucose, 84 DNA, and proteins, 61 , 85 − 87 which are associated with health issues and diseases. Therefore, CDs can provide valuable insights into the diagnostics and early precaution of diseases.…”

Smartphone-Based Fluorescence Detection of Al3+ and H2O Based on the Use of Dual-Emission Biomass Carbon Dots

“…CDs have received large amount of attention from various disciplines due to the excellent electrical, optical, and other chemi-physical properties, such as good water solubility, excellent biocompatibility, size-dependent photoluminescence, fluorescence stability, etc. − Since the report of CDs by Xu in 2004, those properties have be widely used in bioimaging, , optical probe, − drug delivery and electrocatalysis . Recently, significant progress has been made on the synthesis and application of multicolor emission CDs. ,− …”