2012
DOI: 10.1186/1471-2164-13-511
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Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development

Abstract: BackgroundDuring sexual development, filamentous ascomycetes form complex, three-dimensional fruiting bodies for the protection and dispersal of sexual spores. Fruiting bodies contain a number of cell types not found in vegetative mycelium, and these morphological differences are thought to be mediated by changes in gene expression. However, little is known about the spatial distribution of gene expression in fungal development. Here, we used laser microdissection (LM) and RNA-seq to determine gene expression … Show more

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Cited by 72 publications
(127 citation statements)
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“…RNA-seq data show that both pheromone receptor genes pre1 and pre2 and the two mating type genes SmtA-1 and SmtA-3 are up-regulated in Δnox1 mycelia and protoperithecia. Similarly, genes for melanin biosynthesis (pks, teh, sdh, and tih) and two genes associated with fruiting body maturation, app and tap1, are down-regulated in Δnox1 mycelia and protoperithecia, similar to the situation in other pro mutants (Nowrousian et al , 2007Teichert et al 2012), whereas they only show minor expression changes in fertile mutants Δnox2 and Δste12 compared to wild type ( Figure 4C). These findings are in line with the proposed hypothesis of a common pro mutant-specific expression pattern.…”
supporting
confidence: 60%
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“…RNA-seq data show that both pheromone receptor genes pre1 and pre2 and the two mating type genes SmtA-1 and SmtA-3 are up-regulated in Δnox1 mycelia and protoperithecia. Similarly, genes for melanin biosynthesis (pks, teh, sdh, and tih) and two genes associated with fruiting body maturation, app and tap1, are down-regulated in Δnox1 mycelia and protoperithecia, similar to the situation in other pro mutants (Nowrousian et al , 2007Teichert et al 2012), whereas they only show minor expression changes in fertile mutants Δnox2 and Δste12 compared to wild type ( Figure 4C). These findings are in line with the proposed hypothesis of a common pro mutant-specific expression pattern.…”
supporting
confidence: 60%
“…Cultivation for ascospore germination assays and DNA extraction were performed as described previously (Nowrousian and Cebula 2005;Kamerewerd et al 2008;Teichert et al 2012). For rescue of fertility, strains were inoculated on filter paper covering solid BMM medium.…”
Section: Methodsmentioning
confidence: 99%
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“…The 18S rRNA gene sequencing was used earlier for species identification in different studies [21]. For the amplification of the SOD-1 gene, a sequence of N. crassa (M58687.1) was used to design the primers because the genomic sequence of N. crassa was found most closely related to S. macrospora than any other sequenced filamentous fungi [22][23]. When SOD-1 gene sequences were aligned with the reference gene of the N. crassa (M58687.1), a total of 25 base substitutions were observed in the exonic region (Fig.…”
Section: Discussionmentioning
confidence: 99%