Combining laser diffraction, flow cytometry and optical microscopy to characterize a nanophytoplankton bloom in the Northwestern Mediterranean

LeRoux, Robin A.; Grégori, Gérald; Leblanc, Karine; Carlotti, François; Thyssen, Mélilotus; Dugenne, Mathilde; Pujo‐Pay, Mireille; Conan, Pascal; Jouandet, Marie-Paule; Bhairy, Nagib; Berline, Léo

doi:10.1016/j.pocean.2017.10.010

Cited by 13 publications

(14 citation statements)

References 42 publications

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“…Data for LISST-Deep derived parameters VC, D50, and occasionally ξ, presented in the current study represent a ∼30% increase relative to that available in the literature (Karageorgis et al, 2012;Karageorgis et al, 2014;Karageorgis et al, 2017b;Parinos et al, 2017;Turner et al, 2017;Leroux et al, 2018). A substantial amount of data have been previously reported with LISST-100X, i.e.…”

Section: Spatial and Vertical Variations Of Particulate Matter Optical Propertiessupporting

confidence: 50%

Optical Properties and Biochemical Indices of Marine Particles in the Open Mediterranean Sea: The R/V Maria S. Merian Cruise, March 2018

et al. 2021

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A rich data set on particulate matter optical properties and parameters (beam attenuation coefficient, volume concentration, particle size and PSD slope), accompanied by measurements of biochemical indices (particulate organic carbon, particulate nitrogen and their stable isotopic composition) was obtained from the surface to deep waters across the Mediterranean Sea, in March-April 2018. A decrease of beam attenuation coefficients, total particle volume concentrations, particulate organic carbon and nitrogen concentrations was noted towards the eastern Mediterranean Sea (EMed) in comparison to the western Mediterranean Sea (WMed). LISST-derived optical properties were significantly correlated with water mass characteristics. Overall, the most turbid water mass identified in the Mediterranean Sea was the Surface Atlantic water (AW), and the most transparent was the Transitional Mediterranean Water (TMW) in the Cretan Sea, whereas a general decrease in particulate matter concentration is observed from the WMed towards the EMed. Relatively depleted δ13C-POC values in the particle pool of the open Mediterranean Sea can be attributed to contribution from terrestrial inputs, mainly via atmospheric deposition. Throughout the entire water column, a significant positive correlation between particle beam attenuation coefficient and particulate organic carbon concentration is observed in the open Mediterranean Sea. Such relationship suggests the predominance of organic particles with biogenic origin. POC concentration and particle median diameter D50 are significantly and negatively correlated both in the WMed and the EMed Sea, confirming that small particles are POC-rich. At depth, a prominent decrease of most measured parameters was observed, with the exception of particle median diameter that increased substantially in the EMed towards the deep sea, suggesting potentially enhanced aggregation processes. The low particle size distribution slope ξ observed in the EMed, corresponding to larger particle populations, supports the above notion. Basin-wide Rayleigh-type isotopic fractionation in vertical profiles of δ15N-PN across the Mediterranean Sea, underlines the differences in the trophic characters of the two sub-basins and highlights the role of circulation changes on biogeochemical parameters and the redistribution of particulate matter as a source of nutrients in the water column.

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Section: Spatial and Vertical Variations Of Particulate Matter Optical Propertiessupporting

confidence: 50%

Optical Properties and Biochemical Indices of Marine Particles in the Open Mediterranean Sea: The R/V Maria S. Merian Cruise, March 2018

et al. 2021

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“…Both particulate attenuation and absorption of surface water were measured continuously with a WetLabs ACS using a flow-through system similar to the setup described in Slade et al (2010) 52 . Phytoplankton cells were counted semi-continuously (one sample every hour) using an automated Cytosense flow cytometer (Cytobuoy, NL), connected to the continuous clean pumping system 53 , 54 . The Cytosense pumped its sample from an isolated chamber of 200 ml filled in less than 30 s. Each sample was driven towards the flow cell by a calibrated peristaltic pump from which volume analyzed were calculated.…”

Section: Methodsmentioning

confidence: 99%

Surface ocean microbiota determine cloud precursors

Sellegri

Nicosia

Freney

et al. 2021

Sci Rep

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One pathway by which the oceans influence climate is via the emission of sea spray that may subsequently influence cloud properties. Sea spray emissions are known to be dependent on atmospheric and oceanic physicochemical parameters, but the potential role of ocean biology on sea spray fluxes remains poorly characterized. Here we show a consistent significant relationship between seawater nanophytoplankton cell abundances and sea-spray derived Cloud Condensation Nuclei (CCN) number fluxes, generated using water from three different oceanic regions. This sensitivity of CCN number fluxes to ocean biology is currently unaccounted for in climate models yet our measurements indicate that it influences fluxes by more than one order of magnitude over the range of phytoplankton investigated.

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“…Oceans cover approximately 70 % of the Earth's surface, and sea spray emissions contribute up to 6 kt yr −1 of particulate matter, making them a major source of primary aerosol in the atmosphere (Lewis and Schwartz, 2004). The majority of the mass associated with sea spray emissions is in the form of coarse-mode sea salt particles.…”

Section: Introductionmentioning

confidence: 99%

Mediterranean nascent sea spray organic aerosol and relationships with seawater biogeochemistry

et al. 2021

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Abstract. The organic mass fraction from sea spray aerosol (SSA) is currently a subject of intense research. The majority of this research is dedicated to measurements in ambient air. However a number of studies have recently started to focus on nascent sea spray aerosol. This work presents measurements collected during a 5-week cruise in May and June 2017 in the central and western Mediterranean Sea, an oligotrophic marine region with low phytoplankton biomass. Surface seawater was continuously pumped into a bubble-bursting apparatus to generate nascent sea spray aerosol. Size distributions were measured with a differential mobility particle sizer (DMPS). Chemical characterization of the submicron aerosol was performed with a time-of-flight aerosol chemical speciation monitor (ToF-ACSM) operating with 10 min time resolution and with filter-based chemical analysis on a daily basis. Using positive matrix factorization analysis, the ToF-ACSM non-refractory organic matter (OMNR) was separated into four different organic aerosol types, identified as primary OA (POANR), oxidized OA (OOANR), methanesulfonic acid type OA (MSA-OANR), and mixed OA (MOANR). In parallel, surface seawater biogeochemical properties were monitored providing information on phytoplankton cell abundance and seawater particulate organic carbon (1 h time resolution) and seawater surface microlayer (SML) dissolved organic carbon (DOC) (on a daily basis). Statistically robust correlations (for n>500) were found between MOANR and nanophytoplankton cell abundance, as well as between POANR, OOANR, and particulate organic carbon (POC). Parameterizations of the contributions of different types of organics to the submicron nascent sea spray aerosol are proposed as a function of the seawater biogeochemical properties for use in models.

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Combining laser diffraction, flow cytometry and optical microscopy to characterize a nanophytoplankton bloom in the Northwestern Mediterranean

Abstract: International audienc

Cited by 13 publications

References 42 publications

Optical Properties and Biochemical Indices of Marine Particles in the Open Mediterranean Sea: The R/V Maria S. Merian Cruise, March 2018

Optical Properties and Biochemical Indices of Marine Particles in the Open Mediterranean Sea: The R/V Maria S. Merian Cruise, March 2018

Surface ocean microbiota determine cloud precursors

Mediterranean nascent sea spray organic aerosol and relationships with seawater biogeochemistry

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