Combining infrared and mode synthesizing atomic force microscopy: Application to the study of lipid vesicles inside Streptomyces bacteria

Methods to probe the formation of biofilms: applications in foods and related surfaces

“…AFM topography and the corresponding AFM-IR map at 1740 cm À1 IR absorption reveal the lipid production of Streptomyces bacteria in the rst 12 hours of culture growth. 134 This indicates that AFM-IR is sensitive to chemicals inside the bacteria cell and at the biolm surface. It is possible to resolve some of the characterization problems, such as detection of untouchable bio-material, bioproduction and material inside the bio-lm during the specic adhesion.…”

Recent advances in hybrid measurement methods based on atomic force microscopy and surface sensitive measurement techniques

“…Here, the obtained amplitudes are related to the strength of the cantilever oscillation. Studies utilizing PTIR have analyzed the chemical information of biological samples, such as proteins, 279,280 lipids and lipid vesicles, 281,282 cells, 283,284 bacteria, 48,279,285 polymer samples and thin lms, 49,286-288 medical devices, 289 drugs, 290,291 nanostructures, [292][293][294] metal-organic frameworks (MOFs), 295 organo-halide perovskites, 296,297 and nano-patterned metals 298 at a resolution up to 50 nm. Here the improved resolution of the PTIR serves to analyze nanoscale regions of relatively thin samples.…”

“…299 Researchers have shown that polymers and self-assembled monolayers (SAMs) can be investigated via resonance enhanced-atomic force microscopy-infrared spectroscopy (RE-AFM-IR). 299,300 Recently, the coupling of mode synthesizing atomic force microscopy (MS-AFM) 301 with PTIR was proposed, 281 vesicles inside of bacteria could be detected and analyzed in situ. 281 This method can potentially be generalized to nanocontainers inside so matter.…”

Polarization effect in tip-enhanced infrared nanospectroscopy studies of the selective Y5 receptor antagonist Lu AA33810

“…There is growing interest in the field of mid-infrared spectroscopy applications for thin objects at the nanoscale level [1,2]. It is widely known that the infrared absorption of a molecule is greatly strengthened when it is immobilized onto, or is localized in close proximity, to a metal surface, such as Ag, Au, Cu, or In [3].…”