Combining cervical and anal-canal specimens for gonorrhea on a single culture plate

Judson, F N; Werness, Bruce A.

doi:10.1128/jcm.12.2.216-219.1980

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…It was as accurate as the method of three full plates in detecting N. gonorrhoeae. The extensive study of Judson and Werness (5), in which females were screened at anal and cervical sites on a single agar plate, and our study of all patients with positive genital Gram stains cultured at genital, anal, and pharyngeal sites on a single plate both support the value of multiple-site culturing on a single plate (5). In our study, detection is enhanced by the screening of all three sites.…”

supporting

confidence: 73%

Cost-effective method of triple-site culturing for Neisseria gonorrhoeae

Gartner¹,

Edwards²

1984

J Clin Microbiol

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The efficacy of the use of a single modified Thayer-Martin triplate versus that of three separate modified Thayer-Martin plates for the recovery of Neisseria gonorrhoeae from pharyngeal, anal, and genital sites were studied. A total of 98 males and 74 females who showed intracellular gram-negative diplococci on genital Gram stain were studied. Of 172 patients, 164 had gonorrhea at one or more sites. In the first group of 45 males and 21 females, a single swab from each site was used to inoculate randomly both a standard plate (100 mm in diameter) and one-third of a triplate. In the second group of 53 males and 53 females, two swabs were used for collection from each site. One of each pair of swabs was randomly inoculated onto a standard plate and a triplate. There was no significant difference between the results obtained by inoculation with single swabs and those obtained by inoculation with two swabs. There were no significant differences between the positivity rates obtained with the triplate and those obtained with three standard plates used at each site. Cost (39% that of three separate plates) and acceptance by clinic and laboratory personnel make the triplate method an economical, accurate, and effective triple-site screening system.

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supporting

confidence: 73%

Cost-effective method of triple-site culturing for Neisseria gonorrhoeae

Gartner¹,

Edwards²

1984

J Clin Microbiol

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“…In comparative studies with multiple media, no medium has achieved 100% isolation rates. Multiple culture specimens reduce the probability of failure to isolate N. gonorrhoeae due to sampling error (4,9). Nonselective media, which tend to optimize gonococcal growth, also may permit luxuriant growth of other microorganisms present at culture sites which sometimes obscure detection of gonococci.…”

Section: Discussionmentioning

confidence: 99%

Comparison of GC-Lect and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae

et al. 1989

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A new selective medium, GC-Lect, was compared with modified Thayer-Martin medium (MTM) for isolation of Neisseria gonorrhoeae. Cultures from 620 sexually transmitted disease clinic patients were directly inoculated onto both media, placed in candle extinction jars, and incubated. N. gonorrhoeae was isolated from 175 (29%) of 607 genital cultures, 3 (3%) of 88 pharyngeal cultures, and 6 (29%) of 21 rectal cultures. Ten cultures were positive only on GC-Lect, and 3 were positive only on MTM. In 3 of the 10 cultures positive only on GC-Lect, overgrowth of a Capnocytophaga sp. may have obscured growth on MTM. In this study, vancomycin-susceptible (MIC, <4.0 ,ug/ml) N. gonorrhoeae was isolated on both media, and none of the isolates missed by either medium were susceptible to vancomycin. No differences were noted between the two media in time required for isolation of N. gonorrhoeae. While isolation rates of N. gonorrhoeae were similar, suppression of nongonococcal bacterial species by GC-Lect was superior to that by MTM. GC-Lect is equal to MTM for detection of N. gonorrhoeae and is superior for suppression of normal flora.

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“…Although such strains may be inhibited in vitro by the concentrations of vancomycin used in most selective media (2 to 4 ,ug/ml), the inhibitory effect is inoculum dependent (19, 23, 24, 25a, 27), and the practical significance of this phenomenon for the isolation of N. gonorrhoeae remains unclear. In addition, it has been suggested (2,4,(10)(11)(12) that dual plating of endocervical or urethral specimens significantly enhances the isolation rate. To reexamine these issues, we conducted a study in an urban sexually transmitted disease (STD) clinic to compare the rates of isolation of N. gonorrhoeae from the urethra or endocervix with modified Thayer-Martin medium (MTM), a vancomycin-free selective medium (VFSM), and noninhibitory chocolate agar (CA).…”

mentioning

confidence: 99%

Isolation of Neisseria gonorrhoeae on selective and nonselective media in a sexually transmitted disease clinic

Bonin¹,

Tanino²,

Handsfield³

1984

J Clin Microbiol

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To assess the practical significance of reported increases in the prevalence of vancomycin-susceptible strains of Neisseria gonorrhoeae on isolation of this organism, antibiotic-free chocolate agar (CA), modified Thayer-Martin medium (MTM), and a vancomycin-free selective medium (VFSM) were compared in a sexually transmitted disease clinic. Among 326 cervical gonococcal infections detected in a comparison of CA with MTM, 92.0% were detected on CA, compared with 98.2% on MTM (P < 0.001). Similarly, among 306 cervical infections detected in a comparison of MTM and VFSM, 95.8% of infections were detected with VFSM, compared with 98.4% for MTM (P = 0.10). For 1,632 urethral infections in men, all three media were equivalent, with none detecting fewer than 98% of the infections. Compared with a single inoculation, dual inoculation of MTM increased the diagnostic yield by 1.5% for 206 urethral infections and 2.4% for 83 cervical infections. In our clinic population, MTM is superior to CA or VFSM for the diagnosis of genital gonococcal infections, especially in women. The increased yield that accrued from inoculation of both MTM and either of the other media was not sufficiently high to warrant routine use of this practice in our clinic.

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Combining cervical and anal-canal specimens for gonorrhea on a single culture plate

Cited by 9 publications

References 8 publications

Cost-effective method of triple-site culturing for Neisseria gonorrhoeae

Cost-effective method of triple-site culturing for Neisseria gonorrhoeae

Comparison of GC-Lect and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae

Isolation of Neisseria gonorrhoeae on selective and nonselective media in a sexually transmitted disease clinic

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