Comparison of GC-Lect and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae

Reichart, Cindy A.; Rupkey, Lucy M.; Brady, William E.; Hook, Edward W.

doi:10.1128/jcm.27.5.808-811.1989

Cited by 10 publications

(9 citation statements)

References 20 publications

(16 reference statements)

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“…The sensitivity of culture for acute gonorrhoea is high, but decreases to 50% in chronic infections [7]. Poor sampling techniques and improper specimen storage and transport can decrease culture sensitivity further [8–10].…”

Section: Comparison Of Culture and Pcr For Detection Of Neisseria Gomentioning

confidence: 99%

“…A 10‐mL sample of urine was also collected from each patient. The first swab was inoculated, at the bedside, on to a selective medium (chocolate agar plus Isovitalex supplements) and used to make a smear for Gram's‐staining [9,18]. The second swab was placed in a tube containing 500 µL of 10 mM Tris‐HCl, pH 8.0, transported under refrigerated conditions to the molecular biology laboratory, and processed as described previously [16,19].…”

Section: Comparison Of Culture and Pcr For Detection Of Neisseria Gomentioning

confidence: 99%

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Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

Mayta

Calderón

Taverna

et al. 2006

Clinical Microbiology and Infection

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Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.

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Section: Comparison Of Culture and Pcr For Detection Of Neisseria Gomentioning

confidence: 99%

Section: Comparison Of Culture and Pcr For Detection Of Neisseria Gomentioning

confidence: 99%

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

Mayta

Calderón

Taverna

et al. 2006

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

“…Neisseria gonorrhoeae culture sensitivity and recovery rate vary across the different studies, ranging from 15 to 90% in genital sites (Reichart et al . 1989; Benn et al . 2007; Harryman et al .…”

Section: Resultsmentioning

confidence: 99%

“…In our clinic, we used a similar system: DeltaSwab®, a polyester flocked swab that could explain our improved recovery rate in some locations, such as rectum, compared with other studies with considerably lower rates (Reichart et al . 1989). Nevertheless, we only recovered cultures from 25% of genital infections in women, whereas some recent studies have reported a higher culture recovery from this site, ranging from 16·2 to 53·0% (Harryman et al .…”

Section: Resultsmentioning

confidence: 99%

Neisseria gonorrhoeae culture growth rates from asymptomatic individuals with a positive nucleic acid amplification test

Nadal-Barón

Salmerón

García

et al. 2022

Letters in Applied Microbiology

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Gonorrhoea infections are frequently diagnosed at extragenital locations in asymptomatic individuals and are historically related to poor recovery in culture, which hinders antimicrobial susceptibility testing. The aim of this study was to evaluate recovery rates of Neisseria gonorrhoeae by culture among asymptomatic individuals who tested positive by nucleic acid amplification tests between 2018 and 2019 in Barcelona (Spain). In total, 10 396 individuals were tested for N. gonorrhoeae on first‐void urine, rectal, pharyngeal and/or vaginal swabs depending on sexual behaviour. Overall infection prevalence was 5·5% (95% confidence interval [CI] 5·0–5·9). Seven hundred and ten samples were positive corresponding to 567 individuals. The most common site of infection was the pharynx (71·3%), followed by rectum (23·1%) and genitals (4·7%) (P < 0·0001). The N. gonorrhoeae recovery rate in culture, time from positive screening to culture specimen and inoculation delay were calculated. Recovery rate was 21·7% in pharynx, 66·9% in rectum and 37·0% in genitals (25·0% vagina, 71·4% urethra) (P < 0·0001). Median culture collection time was 1 [0; 3] days, and median inoculation delay was 5·01 [4·99–7·99] h, with no impact on N. gonorrhoeae recovery, P = 0·8367 and P = 0·7670, respectively. Despite efforts towards optimizing pre‐analytical conditions, the N. gonorrhoeae recovery rate in asymptomatic individuals is unacceptably low (especially for pharynx), representing a problem for monitoring antimicrobial‐resistant infections.

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“…With the increased culture of pharyngeal specimens, the growth of Capnocytophaga species becomes a potential problem in certain geographic areas. Because of its ability to spread over the surface of the plate by using its gliding motility, it can obscure the growth of pathogenic Neisseria species that may be present (9).…”

Section: Resultsmentioning

confidence: 99%

New selective medium for the isolation of Neisseria gonorrhoeae

1989

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GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens. With stock cultures, vancomycinresistant Staphylococcus epidermidis that grew on modified Thayer-Martin medium (MTM) was inhibited on the new medium. Also, vancomycin-susceptible strains of N. gonorrhoeae were much less inhibited on the new medium than on Martin-Lewis agar or MTM. With oropharyngeal cultures of healthy volunteers, Capnocytophaga species were frequently isolated on MTM from two of three manufacturers but were completely inhibited on GC-Lect. In the clinical study, visible growth of N. gonorrhoeae occurred within 24 h in 72% of the positive cultures on GC-Lect, compared with only 52% on the reference medium. A total of 50 positive cultures were obtained with GC-Lect, compared with 49 obtained with MTM. The selectivity of GC-Lect was superior, with only 19 cultures producing growth of normal fora, compared with 78 cultures on MTM after 24 h of incubation. The selectivity was especially improved on GC-Lect with regard to yeasts (2 versus 30 cultures) and gram-positive cocci (5 versus 31 cultures).

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Comparison of GC-Lect and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae

Cited by 10 publications

References 20 publications

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

Neisseria gonorrhoeae culture growth rates from asymptomatic individuals with a positive nucleic acid amplification test

New selective medium for the isolation of Neisseria gonorrhoeae

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