Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response

Rammal, Hassan; Bour, Camille; Dubus, Marie; Entz, Laura; Aubert, Léa; Gangloff, Sophie C.; Audonnet, Sandra; Bercu, Nicolae B.; Boulmedais, Fouzia; Mauprivez, Cédric; Kerdjoudj, Halima

doi:10.3390/ijms19113458

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…Figure 1H summarizes the effects of agonists and antagonist of CaSR in PMs. Therefore, our data demonstrate the expression and function of the CaSR as a GPCR in macrophages, consistent with previous reports 24,25 …”

Section: Resultssupporting

confidence: 93%

The CaSR/TRPV4 coupling mediates pro‐inflammatory macrophage function

et al. 2023

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Aim: Although calcium-sensing receptor (CaSR) and transient receptor potential vanilloid 4 (TRPV4) channels are functionally expressed on macrophages, it is unclear if they work coordinately to mediate macrophage function. The present study investigates whether CaSR couples to TRPV4 channels and mediates macrophage polarization via Ca 2+ signaling. Methods: The role of CaSR/TRPV4/Ca 2+ signaling was assessed in lipopolysaccharide (LPS)-treated peritoneal macrophages (PMs) from wild-type (WT) and TRPV4 knockout (TRPV4 KO) mice. The expression and function of CaSR and TRPV4 in PMs were analyzed by immunofluorescence and digital Ca 2+ imaging. The correlation factors of M1 polarization, CCR7, IL-1β, and TNFα were detected using q-PCR, western blot, and ELISA. Results: We found that PMs expressed CaSR and TRPV4, and CaSR activationinduced marked Ca 2+ signaling predominately through extracellular Ca 2+ entry, which was inhibited by selective pharmacological blockers of CaSR and TRPV4 channels. The CaSR activation-induced Ca 2+ signaling was significantly attenuated in PMs from TRPV4 KO mice compared to those from WT mice. Moreover, the CaSR activation-induced Ca 2+ entry via TRPV4 channels was inhibited by blocking phospholipases A2 (PLA2)/cytochromeP450 (CYP450) and phospholipase C (PLC)/Protein kinase C (PKC) pathways. Finally, CaSR activation promoted the expression and release of M1-associated cytokines IL-1β and TNFɑ, which were attenuated in PMs from TRPV4 KO mice. Conclusion: We reveal a novel coupling of the CaSR and TRPV4 channels via PLA2/CYP450 and PLC/PKC pathways, promoting a Ca 2+ -dependent M1 macrophage polarization. Modulation of this coupling and downstream pathways may become a potential strategy for the prevention/treatment of immune-related disease.

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Section: Resultssupporting

confidence: 93%

The CaSR/TRPV4 coupling mediates pro‐inflammatory macrophage function

et al. 2023

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“…Additionally, it was suggested that rough surfaces can promote inflammatory responses by mediating macrophage polarization. [42] It was consistent with the qPCR results in Section 3.5, for the expression of iNOS, CD14, and FOS (pro-inflammatory genes) was downregulated in the HAMA/HANB group, while the expression of NR4A1 and Arg-1 (anti-inflammatory genes) was upregulated. Therefore, we inferred that the smooth surface of HAMA/HANB isolated cells and inhibit inflammation, which might be one of the reasons for the acceleration of the wound healing process.…”

Section: Hama/hanb Treatment Prevented Severe Abdominal Adhesionsupporting

confidence: 89%

A Strong Adhesive Biological Hydrogel for Colon Leakage Repair and Abdominal Adhesion Prevention

Mao

Huang

Zhang

et al. 2023

Adv Healthcare Materials

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Colon leakage is one of the most severe complications in abdominal trauma or surgery cases. It can lead to severe abdominal infection and abdominal adhesions, resulting in prolonged hospital stays and increased mortality. In this study, a photosensitive hydrogel is proposed, which can swiftly form a strong adhesion coating on the damaged colon after UV irradiation, to realize quick cure and suture‐free repair of colon leakage. The newly developed biological gel consists of hyaluronic acid methacryloyl (HAMA) and hyaluronic acid o‐nitroso benzaldehyde (HANB) in the optimal ratio of 3: 1, which exerts both the rapid photocuring properties of HAMA and the strong tissue adhesion properties of HANB. HAMA/HANB shows excellent adhesion stability on wet surfaces, presenting controllable mechanical properties, ductility, adhesion stability, and chemical stability; it also evades foreign body response, which relieves the degree of abdominal adhesion. The underlying mechanism for HAMA/HANB promoting wound healing in colon leakage involves the reconstruction of the colon barrier, as well as the regulation of the immune reaction and neovascularization. In all, HAMA/HANB is a promising alternative suture‐free approach for repairing colon leakage; it has a reliable healing effect and is expected to be extended to clinical application for other organ injuries.This article is protected by copyright. All rights reserved

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“…The Hybrid coating possesses excellent bioactivity and capability of inducing an overwhelmingly positive response of stromal cells and monocytes in favor of bone regeneration. [26][27][28]41 Obtained by a versatile simultaneous spraying of interacting species, this coating of about 1 µm in thickness is composed of amorphous calcium phosphate and carbonated poorly crystalline hydroxyapatite, with a Ca/P ratio close to 1.30, wrapped within chitosan/hyaluronic acid polysaccharide complex (Fig. 1A and B).…”

Section: Resultsmentioning

confidence: 99%