Combined use of gliadins and SSRs to analyse the genetic variability of the Spanish collection of cultivated diploid wheat (Triticum monococcum L. ssp. monococcum)

Abstract

This work studied the combined use of gliadins and SSRs to analyse inter-and intraaccession variability of the Spanish collection of cultivated einkorn {Triticum monococcum L. ssp. monococcum) maintained at the CRF-INIA. In general, gliadin loci presented higher discrimination power than SSRs, reflecting the high variability of the gliadins. The loci on chromosome 6A were the most polymorphic with similar PIC values for both marker systems, showing that these markers are very useful for genetic variability studies in wheat. The gliadin results indicated that the Spanish einkorn collection possessed high genetic diversity, being the differentiation large between varieties and small within them. Some associations between gliadin alleles and geographical and agro-morphological data were found. Agro-morphological relations were also observed in the clusters of the SSRs dendrogram. A high concordance was found between gliadins and SSRs for genotype identification. In addition, both systems provide complementary information to resolve the different cases of intraaccession variability not detected at the agromorphological level, and to identify separately all the genotypes analysed. The combined use of both genetic markers is an excellent tool for genetic resource evaluation in addition to agromorphological evaluation.

The genetic diversity associated with seed proteins in a collection of Spanish underground vetches (Vicia sativa L. subsp. amphicarpa (Dorthes) Asch. et Graebn.)

“…amphicarpa accessions on the basis of their morphological features is complicated given the wide variation and overlapping of these traits (Tardío 1997). Differences in seed storage proteins, however, have proved to be useful in attempts to separate accessions of faba bean, grass pea, wheat, lentil and buckwheat (Pascualini et al 1991;Przybylska et al 2000;Ruiz et al 2007;Routray et al 2007;Scippa et al 2008;Li et al 2008). In contrast to the results of Haider and El-Shanshoury (2000), the accessions of Vicia sativa subsp.…”

Short communication. Collection and characterisation of a population of Triticum boeoticum Boiss., a wild wheat species not previously found in the Mediterranean western region

“…Some agromorphological traits such as very high hairy leaf, and long and less dense spike were clearly different from the Spanish accessions of cultivated einkorn (Fig. 1b), which had hairless leaf, and shorter and very dense spikes (Ruiz et al, 2007). Agro-morphological data also indicated that the sample was different from other T. boeoticum accessions maintained at the CRF-INIA.…”

“…Table 1 shows the agro-morphological data of the accession. The variables were measured according to IBPGR (1985) and Ruiz et al (2007). Some agromorphological traits such as very high hairy leaf, and long and less dense spike were clearly different from the Spanish accessions of cultivated einkorn (Fig.…”

Assessment genetic diversity of einkorn genotypes (Triticum monococcum L.) by gliadin electrophoresis

“…Zhaocai et al (2006) identified 14 and 19 allele variants, respectively at Gli-A1m and Gli-A2m of 41 T. monococcum accessions. Ruiz et al (2007) noted that Gli-A2m was the most polymorphic and the most useful to distinguish between einkorn varieties.…”