Combined use of biocontrol agents and zeolite as a management strategy against Fusarium and Verticillium wilt

Kefalogianni, Io; Gkizi, Danai; Pappa, Eleni; Dulaj, Lorela; Tjamos, Sotiriοs E.; Chatzipavlidis, Iordanis

doi:10.1007/s10526-016-9778-4

Cited by 19 publications

(8 citation statements)

References 51 publications

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“…With the enhancement of people's awareness of environmental protection, the development of biological prevention measures are gaining attention. Pollution-free biological pesticides have become a research hotspot [28,29]. Among all the biological pesticides, biocontrol bacteria or their metabolites are used to control plant diseases, and the work principle is to balance the beneficial and harmful microorganisms around host plants, so as to achieve the purpose of disease prevention and yield increase [30][31][32].…”

Section: Effects Of the Microbial Agents On The Disease Status Of Vermentioning

confidence: 99%

Mixed bacterial fermentation can control the growth and development of Verticillium dahliae

Cheng

Guo

Peng

et al. 2020

Biotechnology & Biotechnological Equipment

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Verticillium wilt is a severe disease caused by Verticillium dahliae, which afflicts many crops, particularly cotton in the Xinjiang province of China. Chemical fungicides are harmful to the environment, and biological control agents against the fungal pathogens of plants provide an alternative to chemicals. Biological control agents include antagonistic bacteria or metabolic products. We investigated the inhibitory effects of two plant-growth-promoting rhizobacterial (PGPR) strains, namely Bacillus tequilensis C-9 and Sphingobacterium A1, against V. dahliae in vitro and in the field. We used the mixed fermentation of two bacteria to inhibit V. dahliae. Antimicrobial assays were performed and showed that spore production and germination, and the virulence protein of V. dahliae were reduced after treatment with cell-free mixed fermentation broth. In field studies, there was improvement in the disease severity, along with declined incidence and index after the treatment in the bud and bell stages. To explore the molecular mechanisms of this inhibition, we studied six metabolism-related genes by real-time quantitative polymerase chain reaction (qRT-PCR). The results showed that in response to mixed fermentation broth, some genes related to growth and development were significantly upregulated, explaining the structural changes observed in V. dahliae. Some genes that regulate oxidative stress were also dramatically induced, indicating that mixed bacterial fermentation had a significant impact on V. dahliae. These results support the idea that the cell-free mixed fermentation broth of Bacillus tequilensis C-9 and Sphingobacterium A1, when applied for biocontrols, can inhibit V. dahliae in vitro and in vivo.Abbreviations: ANOVA: analysis of variance; BE: biocontrol efficacy (%); CFU: colony-forming unit; DI: disease incidence rates (DI %); DS: disease status; PDA: potato dextrose agar; PDB: potato dextrose broth (liquid medium); PGPR: plant-growth-promoting rhizobacteria ARTICLE HISTORY

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Section: Effects Of the Microbial Agents On The Disease Status Of Vermentioning

confidence: 99%

Mixed bacterial fermentation can control the growth and development of Verticillium dahliae

Cheng

Guo

Peng

et al. 2020

Biotechnology & Biotechnological Equipment

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“…Additionally, both F. oxysporum and V. dahliae produce persistent resting structures that survive in the soil for many years in the absence of a susceptible host. Thus far, resistant cultivars, biological control agents, and organic soil amendments are the most effective strategies to control fungal wilt diseases ( Tsuda et al, 2001 ; Suárez-Estrella et al, 2007 ; Markakis et al, 2008 , 2016 ; Angelopoulou et al, 2014 ; Kefalogianni et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Rhizosphere Microbiome Recruited from a Suppressive Compost Improves Plant Fitness and Increases Protection against Vascular Wilt Pathogens of Tomato

et al. 2017

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Suppressive composts represent a sustainable approach to combat soilborne plant pathogens and an alternative to the ineffective chemical fungicides used against those. Nevertheless, suppressiveness to plant pathogens and reliability of composts are often inconsistent with unpredictable effects. While suppressiveness is usually attributed to the compost’s microorganisms, the mechanisms governing microbial recruitment by the roots and the composition of selected microbial communities are not fully elucidated. Herein, the purpose of the study was to evaluate the impact of a compost on tomato plant growth and its suppressiveness against Fusarium oxysporum f. sp. lycopersici (Foxl) and Verticillium dahliae (Vd). First, growth parameters of tomato plants grown in sterile peat-based substrates including 20 and 30% sterile compost (80P/20C-ST and 70P/30C-ST) or non-sterile compost (80P/20C and 70P/30C) were evaluated in a growth room experiment. Plant height, total leaf surface, and fresh and dry weight of plants grown in the non-sterile compost mixes were increased compared to the plants grown in the sterile compost substrates, indicating the plant growth promoting activity of the compost’s microorganisms. Subsequently, compost’s suppressiveness against Foxl and Vd was evaluated with pathogenicity experiments on tomato plants grown in 70P/30C-ST and 70P/30C substrates. Disease intensity was significantly less in plants grown in the non-sterile compost than in those grown in the sterile compost substrate; AUDPC was 2.3- and 1.4-fold less for Foxl and Vd, respectively. Moreover, fungal quantification in planta demonstrated reduced colonization in plants grown in the non-sterile mixture. To further investigate these findings, we characterized the culturable microbiome attracted by the roots compared to the unplanted compost. Bacteria and fungi isolated from unplanted compost and the rhizosphere of plants were sequence-identified. Community-level analysis revealed differential microbial communities between the compost and the rhizosphere, suggesting a clear effect of the plant in the microbiome assembly. Proteobacteria and Actinobacteria were highly enriched in the rhizosphere whereas Firmicutes were strongly represented in both compartments with Bacillus being the most abundant species. Our results shed light on the composition of a microbial consortium that could protect plants against the wilt pathogens of tomato and improve plant overall health.

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“…Supplementary Materials: The following are available online at http://www.mdpi.com/2223-7747/9/11/1622/s1, Table S1: Disease compatibility of specific combinations of race-specific secreted effectors (Ave1 and V2) on resistant cultivars of tomatoes containing different combinations of the race 1 (Ve1) and race 2 (V2) resistance phenotypes, Table S2: Biocontrol agents (BCAs) and biologicals with biocontrol activity against Verticillium dahliae, Table S3: Some examples of organic amendments (OAs) with suppressiveness against Verticillium dahliae, Table S4: Examples of sources of carbon from recent studies for anaerobic soil disinfestation of soil-borne pathogens including Verticillium dahliae. References [137,140,[153][154][155][156][157][158][159][160][161]163,164,166,167,169,174,175,224, are cited in the supplementary materials. Funding: This research was funded by a USDA-NIFA Specialty Crop Research Initiative (SCRI) under award number 2016-51181-25404.…”

Section: Discussionmentioning

confidence: 99%

Opportunities and Challenges in Studies of Host-Pathogen Interactions and Management of Verticillium dahliae in Tomatoes

Acharya

Ingram

et al. 2020

Plants

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Tomatoes (Solanum lycopersicum L.) are a valuable horticultural crop that are grown and consumed worldwide. Optimal production is hindered by several factors, among which Verticillium dahliae, the cause of Verticillium wilt, is considered a major biological constraint in temperate production regions. V. dahliae is difficult to mitigate because it is a vascular pathogen, has a broad host range and worldwide distribution, and can persist in soil for years. Understanding pathogen virulence and genetic diversity, host resistance, and plant-pathogen interactions could ultimately inform the development of integrated strategies to manage the disease. In recent years, considerable research has focused on providing new insights into these processes, as well as the development and integration of environment-friendly management approaches. Here, we discuss the current knowledge on the race and population structure of V. dahliae, including pathogenicity factors, host genes, proteins, enzymes involved in defense, and the emergent management strategies and future research directions for managing Verticillium wilt in tomatoes.

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Combined use of biocontrol agents and zeolite as a management strategy against Fusarium and Verticillium wilt

Cited by 19 publications

References 51 publications

Mixed bacterial fermentation can control the growth and development of Verticillium dahliae

Mixed bacterial fermentation can control the growth and development of Verticillium dahliae

Rhizosphere Microbiome Recruited from a Suppressive Compost Improves Plant Fitness and Increases Protection against Vascular Wilt Pathogens of Tomato

Opportunities and Challenges in Studies of Host-Pathogen Interactions and Management of Verticillium dahliae in Tomatoes

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