Combined treatment with sinomenine and acupuncture on collagen‑induced arthritis through the NF‑κB and MAPK signaling pathway

Xu, Min; Liu, Shaofan; Wan, Ruijie; Chen, Yu

doi:10.3892/ol.2018.8394

Cited by 8 publications

(5 citation statements)

References 29 publications

(35 reference statements)

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“…12 Furthermore, MAPK signaling pathway was reported to be involved in treatment of collagen-induced arthritis by using sinomenine administration combined with acupuncture. 49 Results from these reported studies are consistent with our results of Real-time PCR, indicating that MAPK1 may be the targets of sinomenine.…”

Section: Discussionsupporting

confidence: 89%

Network Pharmacology-Based Approach to Investigate the Molecular Targets of Sinomenine for Treating Breast Cancer

Li¹,

Li²,

Jiang³

et al. 2021

CMAR

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Sinomenine has been known to inhibit the proliferation of breast cancer cells. However, its targets have not been found yet. This study aimed to search for molecular targets of sinomenine for treating breast cancer via network pharmacology. Methods: Potential targets of sinomenine or breast cancer were separately screened from indicated databases. The common targets of both sinomenine and breast cancer were considered as the targets of sinomenine for treating breast cancer. A sinomenine-targetpathway network was constructed based on the obtained results from Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The putative targets of sinomenine were further determined by using protein-protein interaction (PPI) analysis and molecular docking. Finally, the putative targets were verified in vitro and in vivo. Results: Twenty predicted targets were identified through network pharmacological analysis. Gene Ontology (GO) and KEGG pathway enrichment indicated that these predicted targets enriched in the process of MAP kinase activity, VEGF signaling pathway, Relaxin signaling pathway, Growth hormone synthesis, secretion and action. MAPK1, NOS3, NR3C1, NOS1 and NOS2 were further identified as the putative targets by using PPI and molecular docking analysis. Expression of MAPK1, NR3C1, NOS1, NOS2 and NOS3 genes were significantly regulated by sinomenine in both MCF-7 cells and MDA-MB-231 cells. Furthermore, the expression of NR3C1 in human breast cancer specimens was lower than that in para-tumor normal tissues. Meanwhile, the expression of NR3C1 in xenograft tumors was up-regulated after sinomenine treatment. Conclusion: MAPK1, NR3C1, NOS1, NOS2 and NOS3 were identified as the putative targets of sinomenine for treating breast cancer. NR3C1 was preliminarily confirmed as a target of sinomenine in two breast cancer cell lines, xenograft tumor models and human breast cancer specimens. These data indicated that the network pharmacology-based prediction of sinomenine targets for treating breast cancer could be reliable.

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Section: Discussionsupporting

confidence: 89%

Network Pharmacology-Based Approach to Investigate the Molecular Targets of Sinomenine for Treating Breast Cancer

Li¹,

Li²,

Jiang³

et al. 2021

CMAR

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“…The total number of T-lymphocytes is tightly controlled by cytokines. Activated T-lymphocytes are able to act on inflammation through the secretion of IL-17, and stimulation of IL-8 and IL-6 expression ( Kawasaki et al., 2009 ; Minmin et al., 2018 ). Lactobacilli were previously shown to regulate the transcript levels of inflammation related cytokines in previous studies ( Kawasaki et al., 2009 ; Tan et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

Yang

Zhang

et al. 2021

Poultry Science

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We studied the effects of Lactobacillus acidophilus ( L. acidophilus ) on the growth performance, intestinal morphology, barrier function, and immune response of broilers challenged with Escherichia coli O157 ( E. Coli ). A total of 360 1-day-old Cobb male broilers were tested in a 3 × 2 factorial arrangement with 3 dietary L. acidophilus levels (0, 5 × 10 8 CFU/kg, and 10 × 10 8 CFU/kg of diet) and 2 disease challenge treatments (control or E. coli challenged). Results showed that E. coli challenge decreased the ADG, ADFI, and BW of broilers from 15 to 21 d ( P < 0.05), increased the jejunum intestinal wall thickness, and significantly increased the mortality rate. E. coli challenge significantly ( P < 0.05) decreased the serum IgA and IgM contents and peripheral blood CD3+ T cell counts ( P < 0.05), increased the serum CRP, DAO, and LPS levels at 21 d; upregulated the mRNA expression of iNOS, IL-8, IL-1β in the jejunum and iNOS in the spleen, and downregulated the occludin and ZO-1 mRNA expression in the ileum at 21 d compared with uninfected birds ( P < 0.05). Dietary L. acidophilus supplementation consistently showed higher BW, ADG, ADFI, and jejunum and ileum V:C ratio at 14 d and 21 d in the presence and absence of E. coli challenge ( P < 0.05). L. acidophilus supplementation reduced the mortality rate caused by E. coli challenge ( P < 0.05), decreased the serum CRP, DAO, and LPS levels at 14 d and 21 d; upregulated the mRNA expression of occludin and ZO-1 in the jejunum and ileum, and downregulated the mRNA expression of iNOS, IL-8, and IL-1β in the jejunum in E. coli challenged birds at 21 d ( P < 0.05). Dietary supplementation with L. acidophilus can improve the growth performance, intestinal health, and survival of broilers challenged with E. coli .

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“…When cells are stimulated by inflammatory factors, including IL-6 and IL-1β, NF-κB is activated, and P65 enters the nucleus [30,31]. P65 can bind to the nucleotide sequence in the promoter region of the inflammatory factor, and it is involved in the transcription of Tnfa, Il6 and Il1b, thereby enhancing their expression [32,33].…”

Section: Discussionmentioning

confidence: 99%

Acute and Subacute Safety Evaluation of Black Tea Extract (Herbt Tea Essences) in Mice

Ding

Han

et al. 2022

Toxics

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Theabrownin (TB) is a heterogeneous biomacromolecule, extracted from tea, with many functional groups. Importantly, TB possesses diverse health benefits, such as antitumor activity and blood lipid-lowering effects. Presently, the content of TB in tea extract is relatively low. Here, we obtained a deep-processed black tea extract with a high content of TB (close to 80%), which was named Herbt Tea Essences (HTE). Currently, this study was designed to evaluate the biosafety of high-content TB products on mice. We implemented acute and subacute toxic experiments to assess its safety on organs, the serum biochemical and molecular levels. In the acute exposure study, we found that the median lethal dose (LD50) value of HTE was 21.68 g/kg (21.06–24.70 g/kg, greater than 5 g/kg), suggesting that HTE had a low acute toxicity. In the 28-day subacute exposure study, our results showed that no abnormal effects were observed in the 40 and 400 mg/kg/day HTE-treated groups. However, we observed slight nephrotoxicity in the 4000 mg/kg/day HTE-treated group. The HTE-induced nephrotoxic effect might involve the inflammatory response activation mediated by the nuclear transcription factor kappa-B (NF-κB) signaling pathway. This study would provide valuable data for the TB safety assessment and promote this natural biomacromolecule application in daily drinking.

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Combined treatment with sinomenine and acupuncture on collagen‑induced arthritis through the NF‑κB and MAPK signaling pathway

Cited by 8 publications

References 29 publications

Network Pharmacology-Based Approach to Investigate the Molecular Targets of Sinomenine for Treating Breast Cancer

Network Pharmacology-Based Approach to Investigate the Molecular Targets of Sinomenine for Treating Breast Cancer

Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

Acute and Subacute Safety Evaluation of Black Tea Extract (Herbt Tea Essences) in Mice

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