Combined stimulation with cyclic stretching and hypoxia increases production of matrix metalloproteinase-9 and cytokines by macrophages

Oya, Koki; Sakamoto, Naoya; Ohashi, Toshiro; Sato, Masaaki

doi:10.1016/j.bbrc.2011.08.024

Cited by 13 publications

(18 citation statements)

References 29 publications

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“…3A), which agreed with our previous study (Oya, et al, 2011). Levels of MMP-9 were quantified by the relative intensity of bands (Fig.…”

Section: Mmp-9 Production Of Macrophages Exposed To Cyclic Stretchingsupporting

confidence: 91%

“…According to the hypothesis that these conditions induce aberrant changes in macrophage functions as well as morphology, we have investigated the effect of cyclic stretching and low oxygen conditions on macrophages. As a result, we reported that production of MMP-9 from macrophages increased under the combination of 10 % cyclic stretching and 2.2% O 2 , mimicking an oxygen condition in ILT-accumulated AAA walls (Vorp, et al, 2001), compared to static and 5% stretching conditions (Oya, et al, 2011). We also showed that 2.2% O 2 condition affects morphological changes induced by cyclic stretching compared to normal culture condition of 20% O 2 (Oya, et al, 2013).…”

Section: Introductionmentioning

confidence: 75%

“…Production of MMPs by macrophages under these conditions are believed to be implicated in weakening of arterial walls (Vorp, et al, 2001) (Kazi, et al, 2005) (Murdoch, et al, 2005) (Oya, et al, 2011). In the present study, we focused on the effect of oxygen concentrations on MMP production of macrophages subjected to cyclic stretching.…”

Section: Discussionmentioning

confidence: 99%

“…Differentiation of THP-1 to macrophages were induced by culturing cells with 20 µM phorbol 12-myristate 13-acetaste (PMA, Sigma-Aldrich) for 3 days and confirmed by CD14 expression (data not shown), according to our previous studies (Oya, et al, 2011) (Oya, et al, 2013). After PMA treatment, the culture medium was replaced with fresh macrophage-SFM (Invitrogen, USA), and cells were exposed to 10% cyclic stretching at 1 Hz for 24 h under normal (20%O 2 ) or hypoxic low oxygen conditions (1% or 5% O 2 ).…”

Section: Cyclic Stretching Experimentsmentioning

confidence: 99%

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Effect of low oxygen conditions on matrix metalloproteinase-9 production of macrophages subjected to cyclic stretching: involvement of ERK and Rho kinase pathways

Sakamoto

Sadamoto

2017

JBSE

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Macrophages infiltrated in the walls of abdominal aortic aneurysms (AAA) are subjected to cyclic stretching due to pulsatile deformation of blood vessels and low oxygen conditions caused by intraluminal thrombus. These conditions could induce aberrant changes in macrophage functions and lead local weakening of AAA walls. We previously reported that the combination of 10 % cyclic stretching and 2.2% O 2 conditions caused an increase in matrix metalloproteinase-9 (MMP-9) production in macrophages as well as changes in morphological responses to cyclic stretching. In the present study, we investigated the effects of oxygen concentrations on MMP-9 productions and morphological changes of macrophages subjected to cyclic stretching. Macrophages differentiated from THP-1 cells were subjected to 10% cyclic stretching under 5% or 1% O 2 conditions for 24 h, and gelatinolytic activity of MMP-9 in the conditioned medium was assessed by zymography. Cells showed spread and rounded shape under static conditions and elongated and oriented to the direction of stretching after exposure to cyclic stretching, and there were no obvious effects of oxygen concentrations in cell morphology. An O 2 concentration of 5% did not change MMP-9 productions of macrophages in static culture and subjected to cyclic stretching compared to normal cell culture condition of 20% O 2 . In contrast, 1% O 2 condition stimulated MMP-9 production of macrophages both under static culture and cyclic stretching conditions. We also found that treatments of inhibitors for extracellular signal-regulated kinase (ERK) and Rho associated protein kinase (Rho kinase) suppressed the increased MMP-9 productions of macrophages by 1% O 2 condition. These results suggest that lower oxygen conditions such as 1% O 2 stimulate MMP-9 production in macrophages through signaling pathways involving ERK as well as Rho kinase-mediated actin cytoskeletal contractility.

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“…3A), which agreed with our previous study (Oya, et al, 2011). Levels of MMP-9 were quantified by the relative intensity of bands (Fig.…”

Section: Mmp-9 Production Of Macrophages Exposed To Cyclic Stretchingsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Cyclic Stretching Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of low oxygen conditions on matrix metalloproteinase-9 production of macrophages subjected to cyclic stretching: involvement of ERK and Rho kinase pathways

Sakamoto

Sadamoto

2017

JBSE

Self Cite

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“…These macrophages produced interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) leading to increased apoptosis of vascular smooth muscle cells. Hypoxia was also demonstrated to augment the expression of MMP-1, MT-1 MMP, MMP-2, MMP-7 and MMP-9 in SMC derived from human aorta [12,13].…”

Section: Metalloproteinases and Aneurysmsmentioning

confidence: 89%

The Role of Metalloproteinases in the Development of Aneurysm

Siemianowicz¹

2012

Aneurysm

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Biophysical regulation of macrophages in health and disease

Meli

Veerasubramanian

Atcha

et al. 2019

Journal of Leukocyte Biology

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Macrophages perform critical functions for homeostasis and immune defense in tissues throughout the body. These innate immune cells are capable of recognizing and clearing dead cells and pathogens, and orchestrating inflammatory and healing processes that occur in response to injury. In addition, macrophages are involved in the progression of many inflammatory diseases including cardiovascular disease, fibrosis, and cancer. Although it has long been known that macrophages respond dynamically to biochemical signals in their microenvironment, the role of biophysical cues has only recently emerged. Furthermore, many diseases that involve macrophages are also characterized by changes to the tissue biophysical environment. This review will discuss current knowledge about the effects of biophysical cues including matrix stiffness, material topography, and applied mechanical forces, on macrophage behavior. We will also describe the role of molecules that are known to be important for mechanotransduction, including adhesion molecules, ion channels, as well as nuclear mediators such as transcription factors, scaffolding proteins, and epigenetic regulators. Together, this review will illustrate a developing role of biophysical cues in macrophage biology, and also speculate upon molecular targets that may potentially be exploited therapeutically to treat disease.

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Combined stimulation with cyclic stretching and hypoxia increases production of matrix metalloproteinase-9 and cytokines by macrophages

Cited by 13 publications

References 29 publications

Effect of low oxygen conditions on matrix metalloproteinase-9 production of macrophages subjected to cyclic stretching: involvement of ERK and Rho kinase pathways

Effect of low oxygen conditions on matrix metalloproteinase-9 production of macrophages subjected to cyclic stretching: involvement of ERK and Rho kinase pathways

The Role of Metalloproteinases in the Development of Aneurysm

Biophysical regulation of macrophages in health and disease

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