Combined impact of TiO2 nanoparticles and antibiotics on the activity and bacterial community of partial nitrification system

Han, Xu; Liu, Binghua; Qi, Wen-Yu; Xu, Meng; Cui, Xiaoyu; Jun, Liu; Li, Qiang

doi:10.1371/journal.pone.0259671

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…TiO 2 NPs are recognized as highly versatile nanoparticles and are used in coating reagents, cosmetics, medicine, and numerous other industries (Fan et al, 2023; Qiu et al, 2021; Sun et al, 2017). TiO 2 NPs mainly exhibit three crystal forms, namely, anatase, rutile, and brookite.…”

Section: Discussionmentioning

confidence: 99%

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Wang,

Yang,

et al. 2024

J of Applied Toxicology

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Titanium dioxide nanoparticles (TiO2 NPs) can cause apoptosis in TM4 cells; however, the underlying mechanism has not been entirely elucidated. The purpose of this study was to investigate the effects of TiO2 NPs on ROS, Ca2+ level, p38/AKT/mTOR pathway, and apoptosis in TM4 cells and to evaluate the role of Ca2+ in p38/AKT/mTOR pathway and apoptosis. After exposure to different concentrations (0, 50, 100, 150, and 200 μg/mL) of TiO2 NPs for 24 h, cell viability, ROS, Ca2+ level, Ca2+‐ATPase activity, p38/AKT/mTOR pathway‐related proteins, apoptosis rate, and apoptosis‐related proteins (Bax, Bcl‐2, Caspase 3, Caspase 9, and p53) were detected. The ROS scavenger NAC was used to determine the effect of ROS on Ca2+ level. The Ca2+ chelator BAPTA‐AM was used to evaluate the role of Ca2+ in p38/AKT/mTOR pathway and apoptosis. TiO2 NPs significantly inhibited cell viability, increased ROS level, and elevated Ca2+ level while suppressing Ca2+‐ATPase activity. TiO2 NPs regulated the p38/AKT/mTOR pathway via increasing p‐p38 level and decreasing p‐AKT and p‐mTOR levels. TiO2 NPs significantly enhanced the apoptosis. NAC attenuated Ca2+ overload and reduction in Ca2+‐ATPase activity caused by TiO2 NPs. BAPTA‐AM alleviated TiO2 NPs‐induced abnormal expression of p38/AKT/mTOR pathway‐related proteins. BAPTA‐AM assuaged the apoptosis caused by TiO2 NPs. Altogether, this study revealed that TiO2 NPs elevated intracellular Ca2+ level through ROS accumulation. Subsequently, the heightened intracellular Ca2+ level was observed to exert regulation over the p38/AKT/mTOR pathway, ultimately culminating in apoptosis. These results provides a complementary understanding to the mechanism of TiO2 NPs‐induced apoptosis in TM4 cells.

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Section: Discussionmentioning

confidence: 99%

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Wang,

Yang,

et al. 2024

J of Applied Toxicology

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“…The combination of TiO 2 NPs with antibiotics has been revealed to be advantageous in improving antibacterial activity. Xu et al [ 78 ] have demonstrated that TiO 2 NPs at a low level had minimal effects on a wastewater treatment plant, whereas, in combination with tetracycline and erythromycin, the impact of antibiotics was enhanced. This enhanced antibiotic toxicity effect was due to the increasing cell permeability induced by nano-titania.…”

Section: Tio 2 Np Antibacterial Activitymentioning

confidence: 99%

Nanoparticle Impact on the Bacterial Adaptation: Focus on Nano-Titania

Ammendolia

Berardis

2022

Nanomaterials

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Titanium dioxide nanoparticles (nano-titania/TiO2 NPs) are used in different fields and applications. However, the release of TiO2 NPs into the environment has raised concerns about their biosafety and biosecurity. In light of the evidence that TiO2 NPs could be used to counteract antibiotic resistance, they have been investigated for their antibacterial activity. Studies reported so far indicate a good performance of TiO2 NPs against bacteria, alone or in combination with antibiotics. However, bacteria are able to invoke multiple response mechanisms in an attempt to adapt to TiO2 NPs. Bacterial adaption arises from global changes in metabolic pathways via the modulation of regulatory networks and can be related to single-cell or multicellular communities. This review describes how the impact of TiO2 NPs on bacteria leads to several changes in microorganisms, mainly during long-term exposure, that can evolve towards adaptation and/or increased virulence. Strategies employed by bacteria to cope with TiO2 NPs suggest that their use as an antibacterial agent has still to be extensively investigated from the point of view of the risk of adaptation, to prevent the development of resistance. At the same time, possible effects on increased virulence following bacterial target modifications by TiO2 NPs on cells or tissues have to be considered.

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TiO₂ nanopowder and nanofilm catalysts in the disinfection and mineralization of S. aureus with solar-simulated radiation

Shqier,

Zyoud,

Helal

et al. 2024

React. Chem. Eng.

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Combined impact of TiO2 nanoparticles and antibiotics on the activity and bacterial community of partial nitrification system

Cited by 3 publications

References 51 publications

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Nanoparticle Impact on the Bacterial Adaptation: Focus on Nano-Titania

TiO₂ nanopowder and nanofilm catalysts in the disinfection and mineralization of S. aureus with solar-simulated radiation

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Combined impact of TiO2 nanoparticles and antibiotics on the activity and bacterial community of partial nitrification system

Cited by 3 publications

References 51 publications

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca2+‐p38/AKT/mTOR pathway in TM4 cells

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca2+‐p38/AKT/mTOR pathway in TM4 cells

Nanoparticle Impact on the Bacterial Adaptation: Focus on Nano-Titania

TiO2 nanopowder and nanofilm catalysts in the disinfection and mineralization of S. aureus with solar-simulated radiation

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Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

TiO₂ nanopowder and nanofilm catalysts in the disinfection and mineralization of S. aureus with solar-simulated radiation