Combined hypoxia and high temperature affect differentially the response of antioxidant enzymes, glutathione and hydrogen peroxide in the white shrimp Litopenaeus vannamei

Estrada-Cárdenas, Paulina; Cruz-Moreno, Dalia G.; González-Ruiz, Ricardo; Peregrino‐Uriarte, Alma B.; Leyva‐Carrillo, Lilia; Camacho‐Jiménez, Laura; Quintero-Reyes, Idania E.; Yépiz-Plascencia, Glória

doi:10.1016/j.cbpa.2021.110909

Cited by 28 publications

(8 citation statements)

References 51 publications

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“…GSH is an antioxidant that acts on ROS [ 57 ], maintains the balance of oxidative stress and the antioxidant system, and converts GSSG to GSH via GR [ 58 ]. In L. vannamei , GSH has a regulatory role in temperature stress [ 4 ]. In the present study, DEGs were significantly enriched in glutathione metabolism, and the GSH content and GR activity were increased in the low-temperature group.…”

Section: Discussionmentioning

confidence: 99%

“…It can inhibit individual growth or even lead to death at low temperatures [ 1 , 2 ]. When the water temperature changes beyond the tolerance temperature range of poikilothermic animals, oxygen free radicals increase, antioxidant-enzyme activity decreases, oxidative damage is aggravated, and immunity becomes suppressed in the organism [ 3 , 4 ]. To adapt to the drastic changes in the surrounding environment, individuals have evolved a series of temperature stress response mechanisms, such as unfolded-protein response and apoptosis [ 5 ], activation of desaturases [ 6 , 7 ], and increased lipid metabolism [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Low Temperature on Antioxidant and Heat Shock Protein Expression Profiles and Transcriptomic Responses in Crayfish (Cherax destructor)

Yang

Jiang

et al. 2022

Antioxidants

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Low temperature is a critical factor restricting the growth and survival of aquatic animals, but research on the mechanism of response to low temperature in Cherax destructor is limited. C. destructor is one of the most important freshwater crustaceans with strong adaptability in Australia, and it has been commercialized gradually in recent years. Here, growth indicators, antioxidant parameters, anti-stress gene expression, and transcriptome sequencing were used on crayfish following 8 weeks of low-temperature acclimation. The results showed that weight gain, length gain, and molting rates decreased as the temperature decreased. The activity of antioxidant enzymes decreased, while the content of antioxidant substances and the expression of anti-stress genes increased. Transcriptome sequencing identified 589 differentially expressed genes, 279 of which were upregulated and 310 downregulated. The gene functions and pathways for endocrine disorders, glucose metabolism, antioxidant defense, and immune responses were identified. In conclusion, although low-temperature acclimation inhibited the basal metabolism and immune ability of crayfish, it also increased the antioxidant substance content and anti-stress-gene expression to protect the organism from low-temperature damage. This study provided molecular insights into the study of low-temperature responses of low-temperature-tolerant crustacean species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Low Temperature on Antioxidant and Heat Shock Protein Expression Profiles and Transcriptomic Responses in Crayfish (Cherax destructor)

Yang

Jiang

et al. 2022

Antioxidants

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“…Increased oxidoreductases during stressful conditions have been demonstrated in crustaceans [ 28 , 29 ]. For instance, the L. vannamei GPx increase, by effect of hypoxia, led to reoxygenation and high temperature stress in a conditional and specific way [ 55 , 56 , 57 ]. Specifically, changes in GSH occurred in C. quadricarinatus exposed to stressful conditions [ 27 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

cDNA Characterization and Expression of Selenium-Dependent CqGPx3 Isoforms in the Crayfish Cherax quadricarinatus under High Temperature and Hypoxia

Hernández-Aguirre

Fuentes-Sidas

Rivera-Rangel

et al. 2022

Genes

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Glutathione peroxidase 3 (GPx3) is the only extracellular selenoprotein (Sel) that enzymatically reduces H2O2 to H2O and O2. Two GPx3 (CqGPx3) cDNAs were characterized from crayfish Cherax quadricarinatus. The nerve cord CqGPx3a isoform encodes for a preprotein containing an N-terminal signal peptide of 32 amino acid residues, with the mature Sel region of 192 residues and a dispensable phosphorylation domain of 36 residues. In contrast, the pereiopods CqGPx3b codes for a precursor protein with 19 residues in the N-terminal signal peptide, then the mature 184 amino acid residues protein and finally a Pro-rich peptide of 42 residues. CqGPx3 are expressed in cerebral ganglia, pereiopods and nerve cord. CqGPx3a is expressed mainly in cerebral ganglia, antennulae and nerve cord, while CqGPx3b was detected mainly in pereiopods. CqGPx3a expression increases with high temperature and hypoxia; meanwhile, CqGPx3b is not affected. We report the presence and differential expression of GPx3 isoforms in crustacean tissues in normal conditions and under stress for high temperature and hypoxia. The two isoforms are tissue specific and condition specific, which could indicate an important role of CqGPx3a in the central nervous system and CqGPx3b in exposed tissues, both involved in different responses to environmental stressors.

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“…This response to increased antioxidant levels in low oxygen conditions is called preparation for oxidative stress (Oliveira et al, 2018). This adaptive mechanism refers to the increased expression and/or activity of antioxidant enzymes during hypoxia, which may attenuate the effects of increased ROS formation during hypoxia (Estrada-Cárdenas et al, 2021). However, it has been suggested that increased mitochondrial ROS formation induced by hypoxia may trigger the activation of antioxidant defenses during hypoxia limitation (Welker et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death

et al. 2022

Front. Physiol.

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Acute hypoxia in water has always been a thorny problem in aquaculture. Oxygen and iron play important roles and are interdependent in fish. Iron is essential for oxygen transport and its concentration tightly controlled to maintain the cellular redox homeostasis. However, it is still unclear the role and mechanism of iron in hypoxic stress of fish. In this study, we investigated the role of iron in hypoxic responses of two zebrafish-derived cell lines. We found hypoxia exposed zebrafish liver cells (ZFL) demonstrated reduced expression of Ferritin and the gene fth31 for mitochondrial iron storage, corresponding to reduction of both intracellular and mitochondrial free iron and significant decrease of ROS levels in multiple cellular components, including mitochondrial ROS and lipid peroxidation level. In parallel, the mitochondrial integrity was severely damaged. Addition of exogenous iron restored the iron and ROS levels in cellular and mitochondria, reduced mitochondrial damage through enhancing mitophagy leading to higher cell viability, while treated the cells with iron chelator (DFO) or ferroptosis inhibitor (Fer-1) showed no improvements of the cellular conditions. In contrast, in hypoxia insensitive zebrafish embryonic fibroblasts cells (ZF4), the expression of genes related to iron metabolism showed opposite trends of change and higher mitochondrial ROS level compared with the ZFL cells. These results suggest that iron homeostasis is important for zebrafish cells to maintain mitochondrial integrity in hypoxic stress, which is cell type dependent. Our study enriched the hypoxia regulation mechanism of fish, which helped to reduce the hypoxia loss in fish farming.

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Combined hypoxia and high temperature affect differentially the response of antioxidant enzymes, glutathione and hydrogen peroxide in the white shrimp Litopenaeus vannamei

Cited by 28 publications

References 51 publications

Effects of Low Temperature on Antioxidant and Heat Shock Protein Expression Profiles and Transcriptomic Responses in Crayfish (Cherax destructor)

Effects of Low Temperature on Antioxidant and Heat Shock Protein Expression Profiles and Transcriptomic Responses in Crayfish (Cherax destructor)

cDNA Characterization and Expression of Selenium-Dependent CqGPx3 Isoforms in the Crayfish Cherax quadricarinatus under High Temperature and Hypoxia

Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death

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