Effects of Low Temperature on Antioxidant and Heat Shock Protein Expression Profiles and Transcriptomic Responses in Crayfish (Cherax destructor)

Abstract

Low temperature is a critical factor restricting the growth and survival of aquatic animals, but research on the mechanism of response to low temperature in Cherax destructor is limited. C. destructor is one of the most important freshwater crustaceans with strong adaptability in Australia, and it has been commercialized gradually in recent years. Here, growth indicators, antioxidant parameters, anti-stress gene expression, and transcriptome sequencing were used on crayfish following 8 weeks of low-temperature acclimation. The results showed that weight gain, length gain, and molting rates decreased as the temperature decreased. The activity of antioxidant enzymes decreased, while the content of antioxidant substances and the expression of anti-stress genes increased. Transcriptome sequencing identified 589 differentially expressed genes, 279 of which were upregulated and 310 downregulated. The gene functions and pathways for endocrine disorders, glucose metabolism, antioxidant defense, and immune responses were identified. In conclusion, although low-temperature acclimation inhibited the basal metabolism and immune ability of crayfish, it also increased the antioxidant substance content and anti-stress-gene expression to protect the organism from low-temperature damage. This study provided molecular insights into the study of low-temperature responses of low-temperature-tolerant crustacean species.

Effects of temperature and salinity on tissue structure, antioxidant capacity and heat shock protein gene expression of Penaeus monodon

“…In Yang,experiment, the expression of HSP60 and HSP70 was signi cantly upregulated, while other HSPs decreased or had no signi cant change at low temperatures in cray sh. His results indicated that HSP60 and HSP70 might have stronger protective effects at lowtemperature acclimation in C. destructor [33]. In our experiment, mRNA expression of most hsps showed a low level of decline under low salinity conditions, which was consistent with the experimental results of Bao et alTherefore, the hepatopancreas exhibit a sensitive HSPs response to promote immune process adaptation to salinity changes.These results suggest that the contribution of energy metabolism to osmotic regulation may lead to the decrease of HSP expression in gill of Apostichopus japonicus under low salinity stress.…”

Efektivitas pakan moist berbasis singkong dan keong pada budidaya lobster air tawar (Cherax quadricarinatus)

“…Suhu merupakan parameter kunci dalam kualitas perairan karena dapat berdampak langsung terhadap metabolisme LAT dan dapat mempengaruhi kualitas perairan lain. Suhu juga dapat berpengaruh terhadap pertumbuhan dan molting pada krustase, termasuk LAT (Yang et al 2022). Dalam penelitian (Yang et al (2022) dan García-Guerrero et al (2013) diketahui bahwa suhu ideal bagi budidaya LAT adalah 25 -30 0 C, meskipun mereka tetap dapat bertahan pada suhu 23 -26 0 C.…”

“…Suhu juga dapat berpengaruh terhadap pertumbuhan dan molting pada krustase, termasuk LAT (Yang et al 2022). Dalam penelitian (Yang et al (2022) dan García-Guerrero et al (2013) diketahui bahwa suhu ideal bagi budidaya LAT adalah 25 -30 0 C, meskipun mereka tetap dapat bertahan pada suhu 23 -26 0 C.…”

Effects of Cold Stress on the Hemolymph of the Pacific White Shrimp Penaeus vannamei

“…Penaeus vannamei, commonly known as whiteleg shrimp, has become one of the most productive aquaculture species in the world due to its significant economic value [1]. Water temperature changes are common stressors in aquaculture; among these stressors, low temperature is a serious environmental stress factor [2][3][4]. Low temperatures can affect shrimp health by suppressing the immune system and disrupting physiological processes [5][6][7].…”