Combined Femtosecond Laser Glass Microprocessing for Liver-on-Chip Device Fabrication

Abstract

Nowadays, lab-on-chip (LOC) devices are attracting more and more attention since they show vast prospects for various biomedical applications. Usually, an LOC is a small device that serves a single laboratory function. LOCs show massive potential for organ-on-chip (OOC) device manufacturing since they could allow for research on the avoidance of various diseases or the avoidance of drug testing on animals or humans. However, this technology is still under development. The dominant technique for the fabrication of such devices is molding, which is very attractive and efficient for mass production, but has many drawbacks for prototyping. This article suggests a femtosecond laser microprocessing technique for the prototyping of an OOC-type device—a liver-on-chip. We demonstrate the production of liver-on-chip devices out of glass by using femtosecond laser-based selective laser etching (SLE) and laser welding techniques. The fabricated device was tested with HepG2(GS) liver cancer cells. During the test, HepG2(GS) cells proliferated in the chip, thus showing the potential of the suggested technique for further OOC development.

Complex in vitro model: A transformative model in drug development and precision medicine

“…Its limited drug adsorption properties render it appropriate for drug investigations that necessitate precise maintenance of drug concentrations. 74 Polymethylmethacrylate, a costeffectiveness transparent material possessing favorable optical attributes and low drug adsorption properties, is widespread used in microfluidic devices and drug compatibility studies. Besides, materials like natural biomaterials (represented by gelatin, alginate, and collagen), PLGA, et al are used to fabricate microfluidic chips.…”

“…Glass, on the other hand, is a frequently used material that exhibits remarkable chemical resistance and optical transparency. Its limited drug adsorption properties render it appropriate for drug investigations that necessitate precise maintenance of drug concentrations 74 . Polymethylmethacrylate, a cost‐effectiveness transparent material possessing favorable optical attributes and low drug adsorption properties, is widespread used in microfluidic devices and drug compatibility studies.…”

Effect of varied beam diameter of picosecond laser on Foturan glass volume microprocessing

“…In the last decade, fabrication of three-dimensional (3D) microstructures inside the transparent materials using ultrashort laser pulses encountered a significant progress in fundamental and applied science, opening up new paths in a vast range of applications [1][2][3][4][5]. The implementation of such a fascinating tool made possible the fabrication of multilayered optofluidic elements [6][7][8], optical waveguides [9], 3D optical memory [10,11] and lab-on-a chip devices [12][13][14][15]. In particular, previous studies demonstrating fabrication of hollow microchannels inside the Foturan glass, type of photostructurable glass [16][17][18], ensured the possibility of using them as 3D microfluidics for cell biology [19].…”