Combined exposure to nano-silica and lead induced potentiation of oxidative stress and DNA damage in human lung epithelial cells

Lu, Chunfeng; Yuan, Xiaoyan; Li, Lizhong; Zhou, Wei; Zhao, Jun; Wang, Yimei; Peng, Shuangqing

doi:10.1016/j.ecoenv.2015.09.030

Cited by 57 publications

(29 citation statements)

References 54 publications

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“…After hydrogen peroxide exposure, the SOD activity has been decreased signi cantly, reaching 40% in A549 cells (Figure 8(a)). In contrast to Lu et al [54] study, the SOD activity has not been statistically altered by lung cells exposed to nano-SiO 2 alone compared with control cells.…”

Section: Volcanic Ash E Ect On the Reactive Oxygen Speciescontrasting

confidence: 91%

“…generated in the mitochondria or in the other systems is converted by the SOD into hydrogen peroxide (H 2 O 2 ), which in turn is also converted into water (H 2 O) by catalase or glutathione peroxidase (GPx) [53]. erefore, this antioxidant enzyme is very important to maintain the balance between the production of reactive oxygen species and antioxidants, since it can initiate oxidative chain reactions and lipid peroxidation that cause serious cellular damage [54].…”

Section: Volcanic Ash E Ect On the Reactive Oxygen Speciesmentioning

confidence: 99%

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Biological Impact of Exposure to Extremely Fine-Grained Volcanic Ash

Aguilera

Viteri

Seqqat

et al. 2018

Journal of Nanotechnology

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At the northwestern edge of South America is located Ecuador. This place is a classical example of an active continental margin with widespread active volcanism. Detailed studies about the impact of volcanic ash on human health are still lacking. Therefore, the disease of exposed populations is unknown. The objective of the present investigation was to assess the biological impact of Pichincha volcanic ash on cell culture and inflammation in murine lung tissues that will contribute to the understanding of the hazards. In this study, the in vivo phase was performed in mice C57BL/6 exposed to several doses of volcanic ash (0.5, 1, and 3.75 mg/100 g mouse body weight). The body weight and survival were controlled during seven days of treatment. The expression of inflammation markers NRLP 3, caspase-1, pro-IL-1, IL-1β, IL-6, IL-8, and h-HPRT was analyzed. The in vitro phase was performed in lung cancer cells A549, peritoneal macrophages, and McCoy cells exposing them to different concentrations of volcanic ash (80, 320, and 1280 μg/cm3) to determine the cytotoxicity and the production of reactive oxygen species. The ash initiated activation of the inflammasome complex NRLP 3 and the initiation of a proinflammatory activity in the murine lung tissue depending on the concentration of this agent. The viability of A549 and McCoy cell decreased with the length of exposure and increased with the concentration of volcanic ash. The activity in superoxide dismutase decreased by about 60%, leading to the formation of reactive oxygen species. These results associated with compounds contained in Pichincha volcanic ash are considered hazardous elements which induce inflammation leading to activate inflammasome NRLP, releasing reactive oxygen species, and producing changes in cell morphology and density, all of which are expression of cytotoxicity.

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Section: Volcanic Ash E Ect On the Reactive Oxygen Speciescontrasting

confidence: 91%

Section: Volcanic Ash E Ect On the Reactive Oxygen Speciesmentioning

confidence: 99%

Biological Impact of Exposure to Extremely Fine-Grained Volcanic Ash

Aguilera

Viteri

Seqqat

et al. 2018

Journal of Nanotechnology

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“…16,17 The interaction types were as follows: 1) additive effect: F5, P0.05, and E A×B  E A + E B ; 2) synergistic effect: F5, P0.05, and E A×B  E A + E B ; and 3) antagonistic effect: F5, P0.05, and E A×B  E A + E B . 18…”

Section: Discussionmentioning

confidence: 99%

“…1,2 In addition, SiNPs are the main constituents of atmospheric particulate matter, 3 while lead (Pb) is another well-known toxic environmental air pollutant derived from anthropogenic activities. 4 Nanoparticles possess a unique feature wherein heavy metals could be adsorbed on the surface and subsequently result in adverse toxicity. 5 Concerns about the combined toxicity of atmospheric particulates and pollutants on living organisms are receiving great attention.…”

Section: Introductionmentioning

confidence: 99%

Co-exposure subacute toxicity of silica nanoparticles and lead acetate on cardiovascular system

Lin

Yang

Shi

et al. 2018

IJN

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BackgroundThe harmful effects following the release of nanomaterials into environment are of great concern today.PurposeIn this study, subacute effect due to co-exposure to low-dose silica nanoparticles (SiNPs) and lead acetate (Pb) on cardiovascular system was detected in Sprague Dawley male rats.Materials and MethodsHistopathological and ultrastructural changes of heart, aortic arch and abdominal aorta were detected. Blood routine and blood biochemistry examinations were used to show the changes of blood components. The fibrinolytic and plasmin factors, inflammation-related factors and myocardial-related enzyme in serum were analysised by ELISA and Western blot assay.ResultsHistopathological and ultrastructural examination of heart, aortic arch, and abdominal aorta showed that serious damage occurred in co-exposure group (n=6/group). Blood routine examination showed that leukocytosis and thrombocytopenia increased markedly, while changes in the erythrocyte count were not obvious in the co-exposure group. The expression of alanine transaminase (ALT) decreased obviously in co-exposure group, while no significant changes were noted in the expression of aspartate aminotransferase (AST), cholesterol (CHO), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein-cholesterol (LDL-C) in the co-exposure group on blood biochemistry analysis. In addition, data from ELISA analysis showed that the levels of fibrinolytic and plasmin factors, including thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), tissue-type plasminogen activator (t-PA), tissue factor pathway inhibitor (TFPI), and antithrombin III (AT III), were decreased, while those of human fibrinogen (FIB) and D-dimer (D2D) increased significantly in the co-exposure group. Moreover, the myocardial-related enzyme in serum, tested by ELISA, and cardiovascular-related protein expression of atrial natriuretic peptide and brain natriuretic peptide, tested by Western blot assay, was increased in the heart. Furthermore, the expression of inflammation factors such as C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) was increased in heart tissue subjected to combined exposure, which was manifested by Western blot assay, while the protein levels of angiotensin II (ANG II) and endothelin 1 were (ET-1) elevated in blood vessels in the co-exposure group.ConclusionIn conclusion, the major interactions involved in subacute toxicity due to co-exposure to low doses of SiNPs and Pb on cardiovascular system were expected to be additive and synergistic in nature. Co-exposure to SiNPs and Pb could aggravate the cardiovascular toxicity via endothelial damage, hypercoagulation, and cardiac injury in vivo.

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“…In line with our results, several studies have shown that ZnO NPs or other types of NPs induce DNA damage in a variety of cell types. [50][51][52][53][54][55][56][57][58][59][60] In vivo exposure to ZnO NPs alters the structure of seminiferous tubules and spermatozoa morphologies…”

Section: Zno Nps Induce Nuclear Dna Leakage and Breakagementioning

confidence: 99%

Cytotoxic effects of ZnO nanoparticles on mouse testicular cells

Han¹,

Yan²,

Ge³

et al. 2016

IJN

101

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Nanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model

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Combined exposure to nano-silica and lead induced potentiation of oxidative stress and DNA damage in human lung epithelial cells

Cited by 57 publications

References 54 publications

Biological Impact of Exposure to Extremely Fine-Grained Volcanic Ash

Biological Impact of Exposure to Extremely Fine-Grained Volcanic Ash

Co-exposure subacute toxicity of silica nanoparticles and lead acetate on cardiovascular system

Cytotoxic effects of ZnO nanoparticles on mouse testicular cells

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