Co-exposure subacute toxicity of silica nanoparticles and lead acetate on cardiovascular system

Lin, Feng; Yang, Xiaozhe; Shi, Yanfeng; Liang, Shuang; Zhao, Tong; Duan, Junchao; Sun, Zhiwei

doi:10.2147/ijn.s185259

Cited by 38 publications

(23 citation statements)

References 45 publications

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“…Wu et al [23] observed that co-exposure of nSiO 2 and benzo(a)pyrene causes synergistic toxicity to human bronchial epithelial (BEAS-2B) cells. Co-exposure of nSiO 2 and methylmercury (MeHg) or lead (Pb) induces synergistic cardiac toxicity [24,25]. The nSiO 2 and Pb co-exposure induces joint toxicity to human lung epithelial cells (A549) [26,27].…”

Section: Introductionmentioning

confidence: 99%

Co-Exposure to SiO2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells

Ahamed

Akhtar

Alhadlaq

2019

IJERPH

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Widespread application of silica nanoparticles (nSiO2) and ubiquitous metalloid arsenic (As) may increase their chances of co-exposure to human beings in daily life. Nonetheless, studies on combined effects of nSiO2 and As in human cells are lacking. We investigated the co-exposure effects of nSiO2 and As in human liver (HepG2) and human fibroblast (HT1080) cells. Results showed that nSiO2 did not cause cytotoxicity. However, exposure of As caused oxidative stress and apoptosis in both types of cells. Interesting results were that co-exposure of a non-cytotoxic concentration of nSiO2 significantly augmented the As induced toxicity in both cells. Intracellular level of As was higher in the co-exposure group (nSiO2 + As) than the As group alone, suggesting that nSiO2 facilitates the cellular uptake of As. Co-exposure of nSiO2 and As potentiated oxidative stress indicated by pro-oxidants generation (reactive oxygen species, hydrogen peroxide and lipid peroxidation) and antioxidants depletion (glutathione level, and glutathione reductase, superoxide dismutase and catalase activities). In addition, co-exposure of nSiO2 and As also potentiated mitochondria-mediated apoptosis suggested by increased expression of p53, bax, caspase-3 and caspase-9 genes (pro-apoptotic) and decreased expression of bcl-2 gene (anti-apoptotic) along with depleted mitochondrial membrane potential. To the best of our knowledge, this is the first study showing that co-exposure of nSiO2 and As induced augmentation of oxidative stress and mitochondria-mediated apoptosis in HepG2 and HT1080 cells. Hence, careful attention is required for human health assessment following combined exposure to nSiO2 and As.

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Section: Introductionmentioning

confidence: 99%

Co-Exposure to SiO2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells

Ahamed

Akhtar

Alhadlaq

2019

IJERPH

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“…We attempted similar experiments with B[a]P, but could not detect any genotoxic potential of this substance in RAW 264.7 cells. The synergistic interaction of SiO 2 particles with co-contaminants has been reported in various studies, particularly with metals, such as cadmium, methylmercury, arsenic, and lead [13,14,[40][41][42][43][44], or with B[a]P [10][11][12]. Increased genotoxic potential has been highlighted for SiO 2 and B[a]P in epithelial cells [10,11,44].…”

Section: Discussionmentioning

confidence: 94%

Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents

et al. 2020

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Synthetic amorphous silica (SAS) is used in a plethora of applications and included in many daily products to which humans are exposed via inhalation, ingestion, or skin contact. This poses the question of their potential toxicity, particularly towards macrophages, which show specific sensitivity to this material. SAS represents an ideal candidate for the adsorption of environmental contaminants due to its large surface area and could consequently modulate their toxicity. In this study, we assessed the toxicity towards macrophages and intestinal epithelial cells of three SAS particles, either isolated SiO2 nanoparticles (LS30) or SiO2 particles composed of agglomerated-aggregates of fused primary particles, either food-grade (E551) or non-food-grade (Fumed silica). These particles were applied to cells either alone or in combination with genotoxic co-contaminants, i.e., benzo[a]pyrene (B[a]P) and methane methylsulfonate (MMS). We show that macrophages are much more sensitive to these toxic agents than a non-differenciated co-culture of Caco-2 and HT29-MTX cells, used here as a model of intestinal epithelium. Co-exposure to SiO2 and MMS causes DNA damage in a synergistic way, which is not explained by the modulation of DNA repair protein mRNA expression. Together, this suggests that SiO2 particles could adsorb genotoxic agents on their surface and, consequently, increase their DNA damaging potential.

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“…Table 5 shows that the p(AAPBA-b-PTE) NPs did not greatly affect the blood biochemical indexes of mice compared with the normal group. Lin [49] et al observed the subacute effects of low-dose combined poisoning with silica NPs (SiNPs) and lead acetate (PB) on the cardiovascular system of SD rats through blood routine and blood biochemical analysis. It was also indirectly demonstrated that p(AAPBA-b-PTE)…”

Section: Toxicological Researchmentioning

confidence: 99%

Nanoparticles Prepared From Pterostilbene (PTE): A New Strategy for Reducing Blood Glucose and Improving Diabetic Complications

Zhao

Shi

et al. 2021

Preprint

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Aim: New envisions are put forward on the cross application of plant extracts and biomaterials, especially new conjectures are put forward on glucose lowering nanodrug delivery systems.Study design and methods: In this study, pterostilbene (PTE) was esterified with acryloyl chloride firstly, and then 3-acrylamidophenyl boric acid (AAPBA) and PTE esterified by acryloyl chloride were copolymerized into p(AAPBA-b-PTE). The characterization and structure of its polymer were examined. Additionally, p(AAPBA-b-PTE) nanoparticles and insulin loaded p(AAPBA-b-PTE) nanoparticles were prepared. The properties of pH, temperature and glucose sensitivity were investigated. And tested the drug loading and release of NPs. The nanoparticle toxicity was observed through cell and animal experiments, and the nanoparticle biodegradation process under physiological conditions was also observed. Finally, the effects of NPs on reducing blood sugar, antioxidation and improving micro inflammation were investigated in vivo.Results: Based on PTE, we successfully synthesized p(AAPBA-b-PTE) NPs. The NPs were basically round in shape with sizes between 150 and 250 nm. It has good pH and glucose sensitivity. The entrapment efficiency (EE) of insulin loaded NPs is about 56%, and the drug loading (LC) is about 13%. The highest release of insulin was 70%, and the highest release of PTE was 85%. Meanwhile, the insulin could undergo self-regulation according to the change of glucose concentration, thus achieving an effective and sustained release. Both in vivo and in vitro experiments showed that the NPs were safe and nontoxic. Under physiological conditions, it can be completely degraded within 40 days. Fourteen days after the mice were injected with p(AAPBA-b-PTE) NPs, there were no obvious abnormalities in the heart, liver, spleen, lung, and kidney. Moreover, the NPs can effectively reduce blood glucose, improve the antioxidant capacity and improve the micro inflammation status in mice.Conclusions: Using PTE as raw material, p(AAPBA-b-PTE) NPs were successfully prepared, which can effectively reduce blood glucose, improve antioxidant capacity, and reduce inflammatory response. It provided a new way for the combination of plant extracts and biomaterials to regulate and treat diseases through NPs or other dosage forms.

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Co-exposure subacute toxicity of silica nanoparticles and lead acetate on cardiovascular system

Cited by 38 publications

References 45 publications

Co-Exposure to SiO2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells

Co-Exposure to SiO2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells

Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents

Nanoparticles Prepared From Pterostilbene (PTE): A New Strategy for Reducing Blood Glucose and Improving Diabetic Complications

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