Combined Enzymatic and Antioxidative Treatment Reduces Ischemia-Reperfusion Injury in Rabbit Skeletal Muscle

Neumayer, Christoph; Fügl, A.; Nanobashvili, J.; Blumer, Roland; Punz, A.; Gruber, Helmut; Polterauer, P; Huk, Ihor

doi:10.1016/j.jss.2005.12.005

Cited by 47 publications

(34 citation statements)

References 44 publications

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“…In our previous study, we have shown that ascorbic acid prevents renal I/R injury by inhibiting oxidative stress in the rat kidney [45]. Neumayer et al [46] showed that Phlogenzym, a rutincontaining flavonoid tablet was able to prevent I/R injury in rabbit skletal muscle. Furthermore, it was reported that rutin and L-arginine co-administration show beneficial effects in a rat model of liver I/R injury [47].…”

Section: Discussionmentioning

confidence: 91%

Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney

Korkmaz

Kolankaya

2010

Journal of Surgical Research

122

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Section: Discussionmentioning

confidence: 91%

Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney

Korkmaz

Kolankaya

2010

Journal of Surgical Research

122

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“…53 It has also been shown that rutin inhibits xanthine oxidase enxymes, which play a role in I/R pathogenesis. 54 Neumayer and colleagues 55 reported that a flavonoid tablet containing rutin was able to prevent I/R injury in rabbit skeletal muscle.…”

Section: Discussionmentioning

confidence: 99%

Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury

Korkmaz

Kolankaya

2013

Can J Surg

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Background: Nitric oxide (NO) seems to play an important role during renal ischemia/reperfusion (I/R) injury. We investigated whether rutin inhibits inducible nitric oxide synthase (iNOS) and reduces 3-nitrotyrosine (3-NT) formation in the kidneys of rats during I/R. Methods:Wistar albino rats were nephrectomized unilaterally and, 2 weeks later, subjected to 45 minutes of left renal pedicle occlusion followed by 3 hours of reperfusion. We intraperitoneally administered L-N6-(1-iminoethyl)lysine (L-NIL; 3 mg/kg) for 30 minutes or rutin (1 g/kg) for 60 minutes before I/R. After reperfusion, kidney samples were taken for immunohistochemical analysis of iNOS and 3-NT. We measured plasma nitrite/nitrate and cyclic guanosine monophosphate (cGMP) to evaluate NO levels.Results: Ischemia/reperfusion caused plasma cGMP to increase significantly. Similarly, plasma nitrite/nitrate was elevated in the I/R group compared with the control group. Histochemical staining was positive for iNOS and 3-NT in the I/R group. Pretreatment with L-NIL or rutin significantly mitigated the elevation of plasma cGMP and nitrite/nitrate. These changes in biochemical parameters were also associated with changes in immunohistochemical appearance. Pretreatment with L-NIL or rutin significantly decreased the incidence and severity of iNOS and 3-NT formation in the kidney tissues. Conclusion:Our findings suggest that high activity of iNOS causes renal I/R injury, and that rutin exerts protective effects, probably by inhibiting iNOS.Contexte : L'oxyde nitrique (NO) semble jouer un rôle important durant la lésion d'ischémie/reperfusion (I/R) rénale. Nous avons vérifié si la rutine inhibe l'oxyde nitrique synthase inductible (iNOS) et réduit la formation de 3-nitrotyrosine (3-NT) dans les reins de rats durant l'I/R. Méthodes :Des rats albinos Wistar ont subi une néphrectomie unilatérale avant d'être soumis 2 semaines plus tard à une occlusion du pédicule rénal gauche d'une durée de 45 minutes, suivie de 3 heures de reperfusion. Nous avons administré de la L-N6-(1-iminoéthyl)lysine (L-NIL; 3 mg/kg) par voie intrapéritonéale pendant 30 minutes ou de la rutine (1 g/kg) pendant 60 minutes avant l'I/R. Après la reperfusion, des échantillons de tissu rénal ont été prélevés pour analyse immunohistochi mique de l'iNOS et de la 3-NT. Nous avons mesuré les taux plasmatiques de nitrite/nitrate et de guanosine monophosphate cyclique (cGMP) pour évaluer les taux de NO.

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“…Interestingly, studies focusing on the skeletal muscle mitochondrial dysfunctions demonstrated that such metabolic myopathy participated significantly in hind limb ischemia physiopathology. Importantly, ischemia reperfusion-induced skeletal muscle mitochondrial dysfunction has also been shown to be reversible, this reversibility being associated with improvement in a patient's symptoms and walking distance [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Contralateral Leg as a Control During Skeletal Muscle Ischemia-Reperfusion

Thaveau

Zoll

Bouitbir

et al. 2009

Journal of Surgical Research

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Combined Enzymatic and Antioxidative Treatment Reduces Ischemia-Reperfusion Injury in Rabbit Skeletal Muscle

Cited by 47 publications

References 44 publications

Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney

Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney

Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury

Contralateral Leg as a Control During Skeletal Muscle Ischemia-Reperfusion

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