Combined Effects of X Irradiation and Hyperthermia on CHO Cells for Various Temperatures and Orders of Application

Sapareto, Stephen A.; Hopwood, Larry E.; Dewey, William C.

doi:10.2307/3574816

Cited by 123 publications

(37 citation statements)

References 20 publications

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“…The thermal radiosensitization was maximum for both V79 and C3H-1OT 1/2 cells when simultaneously treated with heat and X-rays. This agrees with other studies using different cell lines (Sapareto et al, 1978;Joshi et al, 1978). In both the V79 and C3H-1OT 1/2 cells, the recovery during an incubation interval at 37°C was larger when heat at 42.0°C followed X-rays, compared to X-rays following heat.…”

Section: Discussionsupporting

confidence: 92%

“…Differences may also be due to the nature of the cell since differences in the thermal sensitivity of normal and transformed or cancerous cells in culture have also been observed (Overgaard et al, 1977;Giovanella et al, 1976; Kase and Hahn, 1975;Muckle and Dickson, 1971). Furthermore, thermotolerance was found to influence cellular heat sensitivity and thermal radiosensitization (Henle et al, 1979;Sapareto et al, 1978;.…”

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confidence: 95%

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Thermal radiosensitization in Chinese hamster (V79) and mouse C3H 10T 1/2 cells. The thermotolerance effect

Raaphorst¹,

Azzam²

1983

Br J Cancer

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Summary The sensitivity of V79 cells and normal or morphologically transformed C3H-1OT 1/2 cells to Xrays, heat or heat plus X-rays was examined. The normal and transformed C3H-1OT 1/2 cell lines were equally sensitive to heat at 42.0°C and radiation. The V79 cells were more heat sensitive. Thermal radiosensitization occurred for all 3 cell lines for the combined heat and radiation treatments and was greatest for simultaneous treatment. Recovery occurred when the treatments were separated by an incubation interval at 37°C. For the V79 cells, recovery was much greater for X-rays preceding heat compared to X-rays following heat. This difference was not as great in the C3H-1OT 1/2 cell lines. The transformed C3H-1OT 1/2 cells were more sensitive compared to the normal for the simultaneous treatment or for heating followed by irradiation.For prolonged heating at 42.0°C, after which thermotolerance occurred in all 3 cell lines, the radiosensitivity still increased as a function of heating time even though no additional cell killing occurred from the heat treatment alone. For heating V79 cells at 41.0°C no further increase in radiosensitivity occurred, as cells became thermotolerant during prolonged heating. Also for the development of thermotolerance during incubation at 37°C between two heat treatments, thermal radiosensitization decreased demonstrating that thermotolerance can affect radiosensitization by hyperthermia.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 95%

Thermal radiosensitization in Chinese hamster (V79) and mouse C3H 10T 1/2 cells. The thermotolerance effect

Raaphorst¹,

Azzam²

1983

Br J Cancer

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“…30 The synergistic interaction between two different modalities is maximal when cells are irradiated during heating. 31 However, when the time interval between the two treatments is increased, the potentiated effect is rapidly reduced within 1 hour. 31 In our study, adenovirus -infected cells were irradiated 48 hours after heat treatment ( virus +heat in Fig 7A ).…”

Section: Discussionmentioning

confidence: 99%

“…31 However, when the time interval between the two treatments is increased, the potentiated effect is rapidly reduced within 1 hour. 31 In our study, adenovirus -infected cells were irradiated 48 hours after heat treatment ( virus +heat in Fig 7A ). Although heatinduced radiosensitization was not expected, we observed an increase in cell death by radiation.…”

Section: Discussionmentioning

confidence: 99%

Gene transfer into human prostate adenocarcinoma cells with an adenoviral vector: Hyperthermia enhances a double suicide gene expression, cytotoxicity and radiotoxicity

Lee¹,

Borrelli

2002

Cancer Gene Ther

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We have previously developed a recombinant adenovirus containing a fusion gene of Escherichia coli cytosine deaminase ( CD ) and herpes simplex virus type 1 thymidine kinase ( HSV -1 TK ) controlled by a cytomegalovirus ( CMV ) enhancer -promoter. This replication -incompetent adenovirus effectively transduced the CD -TK gene into human prostate adenocarcinoma DU -145 or PC -3 cells. Interestingly, heat shock at 418C for 4 hours elevated the level of CD -TK by approximately 5 -to 20 -fold at a multiplicity of infection ( MOI ) of 1. Heat -enhanced expression of CD -TK promoted cytotoxicity by 23 -, 9 -, or 47 -fold in the presence of 50 g / mL ganciclovir ( GCV ), 500 g / mL 5 -fluorocytosine ( 5 -FC ), or 50 g / mL GCV + 500 g / mL 5 -FC, respectively, at an MOI of 1. Moreover, there was an increase in radiosensitivity when adenovirus -infected cells were heated at 418C for 4 hours followed by irradiation in the presence of the prodrugs. Virus + heat + 1 g / mL GCV treatment increased radiosensitivity by a dose -modifying factor ( DMF ) of 2.2, whereas virus + heat + 10 g / mL 5 -FC exposure resulted in a DMF of 2.3. Radiosensitization was clearly enhanced as a result of combined prodrug exposure ( DMF = 4.4 ). Our results suggest that the efficiency in expression of suicide genes from an adenoviral vector used for cytotoxic anticancer therapy could be improved by combining heat treatment with radiation therapy.

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“…Hyperthermia (42-450C) enhances the cytotoxic effects of radiation towards tumour cells both in vitro (Sapareto et al, 1978) and in vivo (Mittal et al, 1984). In addition local hyperthermia shows promise for clinical use (for reviews see Arcangeli et al, 1988;Perez & Meyer, 1985).…”

mentioning

confidence: 99%

Effects of localised tumour hyperthermia on pimonidazole (Ro 03-8799) pharmacokinetics in mice

Walton

Bleehen²,

Workman³

1989

Br J Cancer

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Combined Effects of X Irradiation and Hyperthermia on CHO Cells for Various Temperatures and Orders of Application

Cited by 123 publications

References 20 publications

Thermal radiosensitization in Chinese hamster (V79) and mouse C3H 10T 1/2 cells. The thermotolerance effect

Thermal radiosensitization in Chinese hamster (V79) and mouse C3H 10T 1/2 cells. The thermotolerance effect

Gene transfer into human prostate adenocarcinoma cells with an adenoviral vector: Hyperthermia enhances a double suicide gene expression, cytotoxicity and radiotoxicity

Effects of localised tumour hyperthermia on pimonidazole (Ro 03-8799) pharmacokinetics in mice

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