Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation

Abstract

Soy isoflavones, genistein, daidzein and its metabolite equol, as well as β-carotene have been reported to be effective for maintaining bone health. However, it remains to be elucidated whether combining soy isoflavones with β-carotene is beneficial to bone formation. This study investigated the combined effect of soy isoflavones and β-carotene on the differentiation of MC3T3-E1 preosteoblastic cells. Daidzein and genistein alone did not affect cell growth but increased alkaline phosphatase (ALP) activity. Beta-carotene alone inhibited cell growth and markedly enhanced ALP activity. Soy isoflavones combined with β-carotene resulted in higher ALP activity than treatment with isoflavones or β-carotene alone. We observed significant main effects of β-carotene on the enhanced expression of Runx2, ALP, and ostepontin mRNA, whereas there was a significant main effect of soy isoflavones on the expression of osterix mRNA. To investigate how β-carotene affected osteoblast differentiation, MC3T3-E1 cells were treated with retinoic acid receptor (RAR) pan-antagonist combined with β-carotene. Osteopontin and ALP mRNA expression levels, which were increased following treatment with β-carotene, were significantly suppressed by the RAR pan-antagonist. This suggests treatment with β-carotene enhanced early osteoblastic differentiation, at least in part via RAR signaling. These results indicate that a combination of isoflavones and β-carotene may be useful for maintaining a positive balance of bone turnover by inducing osteoblast differentiation.

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

“…26,27 The ability of b-carotene to induce osteogenic differentiation of stem cells has been reported. [28][29][30] Although there are some recent articles about encapsulation of b-carotene within electrospun bers, [31][32][33] based on our literature survey, no research has been reported on the incorporation of b-carotene within electrospun scaffolds for tissue engineering applications.…”

“…RARs attach to DNA as heterodimers with the retinoid X receptors (RXRs) and consequently modulate retinoic acid-responsive target genes. 28,[39][40][41] Wang et al 2 reported that b-carotene exerted an inhibitory effect on osteoclastogenesis and resorption pit formation through the nuclear factor-kappa B (NF-kB) pathway. To study the osteoinductive effect of b-carotene-containing mats on the differentiation of MSCs, scaffolds (PCL/bC2% and PCL/bC4%, 0.5 Â 0.5 cm 2 ) were seeded with cells (10 4 cell per well) and kept in DMEM with 10% FBS as a non-differential medium for up to 21 days.…”

The effect of native propolis on serum biochemical parameters in weaned piglets

“…The ingredients of propolis that are responsible for elevated level of the ALP enzyme are isoflavonoids, such as daidzein and genistein. Isoflavonoids are natural endocrine active compounds generally considered to promote health, and prevent or slow the onset of certain chronic diseases, such as osteoporosis (NISCHIDE et al, 2015;ZHENG et al, 2017). A number of studies have reported that soy isoflavonoid dose-dependently inhibits bone loss in both female and male osteoporotic animal models (REINWALD and WEAVER, 2006).…”

Caracterização Das Plantas Medicinais E Medicamentos Fitoterápicos Para Tratamento Da Osteoporose Utilizados No Brasil

“…Um estudo mostrou que as isoflavonas da soja promovem a proliferação e diferenciação dos osteoblastos, diminuindo acentuadamente os níveis RANKL, aumentando os níveis de OPG em células osteoblásticas resultando em ativação da via β-catenina/ Wnt (YU et al, 2015). A associação das isoflavonas de soja com β-caroteno também mostrou benefício para a formação óssea com manutenção de um equilíbrio positivo na remodelação óssea, por indução da diferenciação de osteoblastos (NISHIDE et al, 2015). Segundo DIETZ et al (2016) evidências sugerem que os efeitos da isoflavona na saúde óssea pode variar em função da idade e de acordo com REINWALD; WEAVER (2006) sendo mais efetiva em mulheres jovens antes que ocorra a perda dos receptores estrogênicos observada na menopausa.…”