Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

Yazdani, Zahra; Mehrabanjoubani, Pooyan; Rafiei, Alireza; Biparva, Pourya; Kardan, Mostafa

doi:10.1155/2021/1969863

Cited by 14 publications

(15 citation statements)

References 70 publications

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“…The expression of BAX (P = 0.034), BCL2 (P = 0.042), and BAX/BCL2 ratio (P < 0.0001) was found significantly altered after combination therapy in B16-F10 cells in comparison with untreated cells. CAP and CUR treatments were found had no significant effects on the expression of apoptotic genes in L929 normal cells [54].…”

Section: Discussionmentioning

confidence: 78%

Effect of Methotrexate and Linoleic Acid on BAX/BCL2 Ratio in Human Hepatocyte Cell

Kabakçı

Kaplan

Şingirik

et al. 2021

JPRI

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Aims: To investigate the effect of methotrexate and linoleic acid on BAX/BCL 2 ratio in human hepatocyte cell. Study Design: Original Research Article. Place and Duration of Study: The study was carried out in partnership with the Department of Anatomy and Department of Medical Pharmacology of Çukurova University Faculty of Medicine, using the laboratory facilities of the Department of Medical Pharmacology. Methodology: Human hepatocyte cell line (CRL-11233) cells obtained from the American Type Culture Collection Organization (ATCC) were used. Expressions of apoptotic pathway markers, apoptosis inducing factor BAX, BCL2 and BAX/BCL2 were evaluated. All analyzes were examined in four groups (Group 1; control, Group 2; linoleic acid given, Group 3; methotrexate given and Group 4; linoleic acid and methotrexate given). Results: The mean ± standard error values of the obtained results as nanogram / milliliter (ng / ml) are in Group I, Group II, Group III and Group IV, respectively; BAX values, 0.900 ± 0.1864, 1.002 ± 0.2098, 8.352 ± 1.467 and 4.295 ± 1.522, BCL 2 values, 13.93 ± 1.198, 13.92 ± 1.739, 2.938 ± 1.059 and 9.250 ± 1.492 and BAX/BCL2 values 0.065, 0.072, 2.843 and 0.464. Conclusion: While BAX/BCL2 level increased in the group given methotrexate, it decreased in the group given linoleic acid and methotrexate.

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Section: Discussionmentioning

confidence: 78%

Effect of Methotrexate and Linoleic Acid on BAX/BCL2 Ratio in Human Hepatocyte Cell

Kabakçı

Kaplan

Şingirik

et al. 2021

JPRI

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“…The synthesized cDNA was directly used as a template for real‐time quantitative polymerase chain reaction (RT‐qPCR). The primer design was performed by Oligo 7.0 software for BAX, BCL2, and CASP3 genes and glyceraldehyde 3‐phosphate dehydogenase (GAPDH) as a reference housekeeping gene (Yazdani et al, 2021 ). It is noticed that only a specific reverse primer was used for cDNA synthesis.…”

Section: Methodsmentioning

confidence: 99%

“…The optimized PCR conditions were 95°C for 15 min, followed by 45 cycles of 95°C for 15 s, and 60°C for 1 min. The levels of mRNA expression were normalized to GAPDH and were calculated using the 2 −ΔΔC t method (Yazdani et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

The combined effect of fish oil containing Omega‐3 fatty acids and Lactobacillus plantarum on colorectal cancer

Sharifi

Yazdani

Najafi

et al. 2022

Food Science & Nutrition

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Colorectal cancer (CRC) is one of the deadliest malignancies. Recent attempts have indicated the role of diet in the etiology of CRC. Natural dietary compounds such as probiotics and Omega‐3 fatty acids that act synergistically can be beneficial in finding a tremendous solution against CRC. To date, the combined effect of fish oil containing Omega‐3 fatty acids (Omega‐3) and Lactobacillus plantarum (L. plantarum) on CRC has been left behind. We here evaluated the effects of co‐encapsulation of Omega‐3 and probiotic bacteria on CRC cell lines compared to normal cells. Omega‐3 and L. plantarum bacteria were co‐encapsulated in three ways, including gelatin–gum Arabic, gelatin–chitosan, and chitosan–gum Arabic complex coacervate microcapsules. After treatment of cells (Normal [L929] and colorectal [C26]) by L. plantarum, Omega‐3, and microcapsules, viability and growth capacity of cell lines were measured using the MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide) assay. Isolated total RNA was used to evaluate the expression profile of BCL2‐associated X protein (BAX), B‐cell lymphoma 2 (BCL‐2), and Caspase‐3 (CASP3) genes by real‐time polymerase chain reaction (PCR). Statistical analysis was performed with SPSS 25 software. A value of p < .05 was considered statistically significant. The results indicated a significant reduction in cell viability of C26 in a concentration‐dependent manner in the treated cells with all treatments, except gelatin–gum Arabic microcapsules. The messenger RNA (mRNA) expression level of the BAX and CASP3 genes in C26 cells being treated with all treatments significantly increased than in untreated cells, and the expression level of the anti‐apoptotic factor of the BCL‐2 gene decreased in C26 cells simultaneously (p < .05). Although, the combined effect of Omega‐3 and L. plantarum and microcapsulated treatments had no more effect on viability and apoptosis gene expression of cancer cells compared to Omega‐3 or L. plantarum. In conclusion, combination therapy with fish oil containing Omega‐3 and L. plantarum does not improve the anticancer effect of each alone.

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“…10 CAP induces oxidative stress and selective cytotoxicity responses in vitro and in vivo by preferential cell death mechanisms such as apoptosis, cell cycle arrest, autophagy, and necrosis in several cancer cell types, including glioblastoma, breast, cervical, melanoma, colorectal, pancreas, colon, head and neck, and lung. [11][12][13][14][15][16][17][18][19][20][21] A recent study revealed that the combination of CAP and dacarbazine in the melanoma mouse model selectively induces autophagy and apoptosis in melanoma and improved cancer treatment. 22 Another cutting-edge melanoma treatment already commonly used in Europe is electrochemotherapy (ECT).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies to date have shown the critical roles of ROS produced by cold atmospheric pressure plasma (CAP) treatment 10 . CAP induces oxidative stress and selective cytotoxicity responses in vitro and in vivo by preferential cell death mechanisms such as apoptosis, cell cycle arrest, autophagy, and necrosis in several cancer cell types, including glioblastoma, breast, cervical, melanoma, colorectal, pancreas, colon, head and neck, and lung 11–21 . A recent study revealed that the combination of CAP and dacarbazine in the melanoma mouse model selectively induces autophagy and apoptosis in melanoma and improved cancer treatment 22 .…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric pressure plasma treatment combined with starvation increases autophagy and apoptosis in melanoma in vitro and in vivo

Golpour

Alimohammadi

Sohbatzadeh

et al. 2022

Experimental Dermatology

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Despite advances in therapy, malignant melanoma remains a fatal disease. Among several emerging approaches to combat cancer, cold atmospheric pressure plasma (CAP) has shown promising results as a novel antitumor agent in preclinical models so far. The technology mainly relies on the emittance of various reactive oxygen and nitrogen species (ROS/RNS) that are tumor-toxic at high concentrations. Moreover, malignant melanoma has a metabolic dimension that can be targeted by mild starvation.To this end, we investigated the combined effect of starvation and CAP treatment on melanoma in vitro and in vivo. In vitro, starvation+CAP led to cell morphology changes, decreased metabolic activity and increased lipid peroxidation accompanied by apoptosis and DNA fragmentation in murine B16 melanoma cells but not murine non-malignant L929 fibroblasts. This was paralleled by increased apoptosis (Bax, Bcl-2 and Caspase-3) and autophagy (Lc3 and Atg5)-related gene expression. In vivo, starvation reduced tumor burden. Combination with CAP treatment augmented this effect significantly, albeit there was no difference of combination treatment to CAP exposure alone. Interestingly, there was an overall greater increase of Lc3 and Atg5 in the tumor tissue compared to CAP exposure alone, while starvation-induced autophagy-related gene expression was similar to in the combination group. These data collectively suggest that CAP-derived ROS/RNS treatment and autophagy-induction augment antitumor effects in malignant melanoma in vitro and in vivo.

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Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

Cited by 14 publications

References 70 publications

Effect of Methotrexate and Linoleic Acid on BAX/BCL2 Ratio in Human Hepatocyte Cell

Effect of Methotrexate and Linoleic Acid on BAX/BCL2 Ratio in Human Hepatocyte Cell

The combined effect of fish oil containing Omega‐3 fatty acids and Lactobacillus plantarum on colorectal cancer

Cold atmospheric pressure plasma treatment combined with starvation increases autophagy and apoptosis in melanoma in vitro and in vivo

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