Combined effect of a natural flavonoid rutin from Citrus sinensis and conventional antibiotic gentamicin on Pseudomonas aeruginosa biofilm formation

Deepika, M; Thangam, Ramar; Sakthidhasan, Periasamy; Sridhar, Arun; Srinivasan, Sivasubramanian; Ramasamy, Thirumurugan

doi:10.1016/j.foodcont.2018.02.044

Cited by 67 publications

(33 citation statements)

References 56 publications

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“…Moreover, absorption at 824 cm −1 indicated a substituted aromatic ring. The assignments are consistent with those reported by Vu et al [30] and Deepika et al [31].…”

Section: Extraction and Characterization Of The Rut Flavonoidsupporting

confidence: 92%

“…Moreover, absorption at 824 cm −1 indicated a substituted aromatic ring. The assignments are consistent with those reported by Vu et al [30] and Deepika et al [31]. The Differential Scanning Calorimetry (DSC) curve of RUT isolated from D. gardneriana ( Figure 2) showed the presence of three endothermic peaks ranging from 134 to 185 °C, and two exothermic peaks at 230 and 244 °C.…”

Section: Extraction and Characterization Of The Rut Flavonoidsupporting

confidence: 90%

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Role of Rutin in 5-Fluorouracil-Induced Intestinal Mucositis: Prevention of Histological Damage and Reduction of Inflammation and Oxidative Stress

et al. 2020

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Intestinal mucositis, characterized by inflammatory and/or ulcerative processes in the gastrointestinal tract, occurs due to cellular and tissue damage following treatment with 5-fluorouracil (5-FU). Rutin (RUT), a natural flavonoid extracted from Dimorphandra gardneriana, exhibits antioxidant, anti-inflammatory, cytoprotective, and gastroprotective properties. However, the effect of RUT on inflammatory processes in the intestine, especially on mucositis promoted by antineoplastic agents, has not yet been reported. In this study, we investigated the role of RUT on 5-FU-induced experimental intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, RUT-50, RUT-100, RUT-200, Celecoxib (CLX), and CLX + RUT-200 groups. The mice were weighed daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis); malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) concentrations; mast and goblet cell counts; and cyclooxygenase-2 (COX-2) activity, as well as to perform immunohistochemical analyses. RUT treatment (200 mg/kg) prevented 5-FU-induced histopathological changes and reduced oxidative stress by decreasing MDA concentrations and increasing GSH concentrations. RUT attenuated the inflammatory response by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. These results suggest that the COX-2 pathway is one of the underlying protective mechanisms of RUT against 5-FU-induced intestinal mucositis.

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“…Moreover, absorption at 824 cm −1 indicated a substituted aromatic ring. The assignments are consistent with those reported by Vu et al [30] and Deepika et al [31].…”

Section: Extraction and Characterization Of The Rut Flavonoidsupporting

confidence: 92%

Section: Extraction and Characterization Of The Rut Flavonoidsupporting

confidence: 90%

Role of Rutin in 5-Fluorouracil-Induced Intestinal Mucositis: Prevention of Histological Damage and Reduction of Inflammation and Oxidative Stress

et al. 2020

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“…Although some of the extracts had a good potential to prevent attachment, none was able to get rid of pre-formed biofilms after 24 and 48 h. In contrast, most of the extracts had good minimum inhibitory activity against P. aeruginosa in the quantitative minimum inhibitory assay. Pseudomonas aeruginosa is a notorious biofilm producer and its biofilms cause the highest number of acute and chronic infections, especially in the excretory and respiratory systems [66, 67], and it can also colonize medical devices and body implants [12]. The enhancement of biofilm production by some of the extracts confirm initial reports that some natural compounds may promote the growth of microbes [68].…”

Section: Discussionmentioning

confidence: 65%

“…The activity of the plant extracts against biofilms at different stages of development highlights their potential usefulness in clinical applications. Such applications may help to enhance the immunological defense of infected hosts against bacterial cell populations, especially those in biofilms, and subsequent host clearance and reduction of disease symptoms [66]. Some of the active extracts may potentially be developed into herbal products which can be useful in alleviating diarrhoeal symptoms and enhancing productivity when added to livestock feed as replacement to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices

Famuyide

Aro

Fasina

et al. 2019

BMC Complement Altern Med

153

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Background Antimicrobial resistance (AMR) remains an important global health issue but the gap between AMR and development of new antimicrobials is increasing. Plant extracts may have good activity per se or may be sources of effective antimicrobial compounds which can act against planktonic and/or biofilms of pathogens. We determined the antimicrobial efficacy and cytotoxicity of some under-investigated plants from the Myrtaceae family endemic to South Africa. The ability of the plant extracts to inhibit or destroy pre-formed bacterial biofilms was also determined. Methods Based on previous preliminary in vitro screening and on chemotaxonomy, nine species from the Myrtaceae family were selected. The antimicrobial activity of the crude acetone leaf extracts was determined against six common nosocomial pathogens, namely: Gram-positive bacteria ( Bacillus cereus , Enterococcus faecalis , Staphylococcus aureus ), Gram-negative bacteria ( Escherichia coli , Pseudomonas aeruginosa , Salmonella Typhimurium) using a two-fold serial microdilution assay with p-iodonitrotetrazolium violet as growth indicator. The number of antimicrobial compounds present in extracts was determined by bioautography. Cytotoxicity of extracts was determined against Vero kidney cells using a colorimetric tetrazolium-based assay. The total antibacterial activity (TAA) in ml/g and selectivity index (LC 50 /MIC) of the plant extracts were calculated. A modified crystal violet assay was used to determine the antibiofilm activity of the extracts. Results Syzygium legatii , Syzygium masukuense , and Syzygium species A had the best activities against Gram-negative and Gram-positive bacteria (MIC) values ranging from 0.04–0.08 mg/ml. Eugenia erythrophylla had the best MIC (0.02 mg/ml) against Bacillus cereus . Many extracts had relatively low cytotoxicity (LC 50 > 20 μg/ml) leading to reasonable selectivity indices. Three leaf extracts ( Syzygium masukuense , Syzygium species A, and Eugenia natalitia ) were moderately cytotoxic (20 μg/ml < LC 50 < 100 μg/ml). The plant extracts had a good capacity to reduce biofilm formation and good to poor potential to destroy pre-formed biofilms. Conclusions The plant species examined in this study had varying degrees of antibacterial activity against bacterial planktonic and biofilm forms with some having good activity against both forms. Several of these selected species may be potential candidates for further investigation to isolate antimicrobial compounds and to determ...

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“…Moreover, phenolic acids, such as chlorogenic [40] and ferulic acids [41], are able to prevent and limit the formation of biofilms. In the case of cedar, there is a further consideration linked to the presence of rutin, which was reported for its inhibitory power on the biofilm formation of P. aeruginosa [42]. Here, the presence of rutin meant that especially peel of cedar was able to inhibit the formation of biofilm by Ps.…”

Section: Biofilm Formation and Metabolic Activity Of Biofilm Cellsmentioning

confidence: 99%

Polyphenols, Antioxidant, Antibacterial, and Biofilm Inhibitory Activities of Peel and Pulp of Citrus medica L., Citrus bergamia, and Citrus medica cv. Salò Cultivated in Southern Italy

et al. 2019

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The aim of this paper was to study the polyphenols of peel and pulp of three Citrus taxa-Citrus medica, Citrus bergamia, and Citrus medica cv. Salò-cultivated in the Cosenza province, Southern Italy, and to evaluate their antioxidant and antibacterial activity, performed against Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, and Pectobacterium carotovorum. Furthermore, we assessed the inhibitory effect of the extracts on bacterial capacity to form biofilm, and on the metabolic activity of the cells present therein. The results indicated that such extracts could find new potential applications in the field of natural antioxidant and anti-bacterial agents in pharmaceutics, agriculture, and food fields.

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Combined effect of a natural flavonoid rutin from Citrus sinensis and conventional antibiotic gentamicin on Pseudomonas aeruginosa biofilm formation

Cited by 67 publications

References 56 publications

Role of Rutin in 5-Fluorouracil-Induced Intestinal Mucositis: Prevention of Histological Damage and Reduction of Inflammation and Oxidative Stress

Role of Rutin in 5-Fluorouracil-Induced Intestinal Mucositis: Prevention of Histological Damage and Reduction of Inflammation and Oxidative Stress

Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices

Polyphenols, Antioxidant, Antibacterial, and Biofilm Inhibitory Activities of Peel and Pulp of Citrus medica L., Citrus bergamia, and Citrus medica cv. Salò Cultivated in Southern Italy

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