2018
DOI: 10.1299/jbse.18-00137
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Combined automated culture system for tubular structure assembly and maturation for vascular tissue engineering

Abstract: Development of the robust yet practical tissue culture systems for vascular grafting is one of the major challenges for biomedical engineering. The following questions should be solved in priority: (1) flexibility of the tissue reservoir allowing dynamic stretching, (2) seeding of cells and extraction of tissues should be easy and safe, (3) system must allow morphological observation in real time, (4) maintenance of metabolic activity of tissues should be performed automatically. In our study, we attempted to … Show more

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Cited by 6 publications
(4 citation statements)
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“…However, the stacking of dense sheets of cells disrupts the supply of nutrients and oxygen to the interior layer 12 . Micro tissue aggregation is reliable method for tissue formation in required size and we previously developed a method and the bioreactor for its implementation to form tubular tissue of arbitrary diameter and length 13 . However, it is challenging to control the self-assembly of the cells, and no one has yet been able to reproduce a physiological vessel with a correct three-layered structure by this approach.…”
Section: Introductionmentioning
confidence: 99%
“…However, the stacking of dense sheets of cells disrupts the supply of nutrients and oxygen to the interior layer 12 . Micro tissue aggregation is reliable method for tissue formation in required size and we previously developed a method and the bioreactor for its implementation to form tubular tissue of arbitrary diameter and length 13 . However, it is challenging to control the self-assembly of the cells, and no one has yet been able to reproduce a physiological vessel with a correct three-layered structure by this approach.…”
Section: Introductionmentioning
confidence: 99%
“…The presented bioreactor allows cultivating three vessels in parallel. Baba et al [18] present a combined automated culture system for tubular structure assembly with enough space for dynamic stretching. The group achieved this by designing an in vivo-like culture chamber made of polydimethylsiloxane (PDMS).…”
Section: Technical Trends In Bioreactor Designmentioning
confidence: 99%
“…The design of an intelligent bioreactor system must feature various sensory elements, a robust system setup and the possibility to reuse the bioreactor containment for several cultivation processes of TEVGs. Different kinds of TEVG bioreactor setups were presented over the past decades, with perfusion bioreactors as the predominantly‐used model [ 16 , 17 , 18 , 19 , 20 ] Features such as specifically compact [ 18 ] and cost‐efficient [ 20 ] designs promote high scalability.…”
Section: Technical Trends In Bioreactor Designmentioning
confidence: 99%
“…However, the stacking of dense sheets of cells disrupt the supply of nutrients and oxygen to the interior layer 12 . Micro tissue aggregation is reliable method for tissue formation in required size and we previously developed a method and the bioreactor for its implementation to form tubular tissue of arbitrary diameter and length 13 . However, it is challenging to control the self-assembly of the cells, and no one has not yet been able to reproduce a natural vessel with a correct three-layered structure by this approach.…”
Section: Introductionmentioning
confidence: 99%