Combinatorial effect of stem cells derived from mandible and recombinant human bone morphogenetic protein-2

Park, Jun‐Beom; Kim, Ki‐Young; Lee, Won; Kim, Hee‐Sung; Kim, In-Soo

doi:10.1007/s13770-014-0038-3

Cited by 9 publications

(8 citation statements)

References 25 publications

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“…BMP-2 is the most common among the signaling molecules used for bone regeneration. BMP-2 is a powerful osteoinductive factor and has been clinically applied to treat fractures and bone defects [90,91]. In order to make BMP-2 function efficiently in the affected area, the performance of the scaffold is considered important.…”

Section: Biodegradable Scaffolds As Drug Delivery Systemsmentioning

confidence: 99%

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

Aoki

2020

Pharmaceutics

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Regenerative medicine has been widely researched for the treatment of bone defects. In the field of bone regenerative medicine, signaling molecules and the use of scaffolds are of particular importance as drug delivery systems (DDS) or carriers for cell differentiation, and various materials have been explored for their potential use. Although calcium phosphates such as hydroxyapatite and tricalcium phosphate are clinically used as synthetic scaffold material for bone regeneration, biodegradable materials have attracted much attention in recent years for their clinical application as scaffolds due their ability to facilitate rapid localized absorption and replacement with autologous bone. In this review, we introduce the types, features, and performance characteristics of biodegradable polymer scaffolds in their role as DDS for bone regeneration therapy.

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Section: Biodegradable Scaffolds As Drug Delivery Systemsmentioning

confidence: 99%

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

Aoki

2020

Pharmaceutics

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“…BMP-2 is the most well-known signaling molecule. BMP-2 exerts a potent bone anabolic effect and has been clinically applied for treating fractures and bone loss [82,83]. Scaffolds for osteogenesis also serve as carriers in the drug delivery system (DDS) of BMP-2, which is a powerful signaling molecule.…”

Section: Cells For Bone Regeneration and Signaling Moleculesmentioning

confidence: 99%

The Use of Electrospun Organic and Carbon Nanofibers in Bone Regeneration

2020

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There has been an increasing amount of research on regenerative medicine for the treatment of bone defects. Scaffolds are needed for the formation of new bone, and various scaffolding materials have been evaluated for bone regeneration. Materials with pores that allow cells to differentiate into osteocytes are preferred in scaffolds for bone regeneration, and porous materials and fibers are well suited for this application. Electrospinning is an effective method for producing a nanosized fiber by applying a high voltage to the needle tip containing a polymer solution. The use of electrospun nanofibers is being studied in the medical field, and its use as a scaffold for bone regeneration therapy has become a topic of growing interest. In this review, we will introduce the potential use of electrospun nanofiber as a scaffold for bone regenerative medicine with a focus on carbon nanofibers produced by the electrospinning method.

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“…Cell therapy applying mesenchymal stem cells with osteoinductive/osteoconductive scaffold has been used in clinical practices, including sinus augmentation procedures [8]. Moreover, combinatorial effect of stem cells and growth factor was tested in previous researches [9,10]. Additionally, it was suggested that mesenchymal stem cells can be combined with bone morphogenetic protein-2 to enhance bone formation in early healing period [10].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, combinatorial effect of stem cells and growth factor was tested in previous researches [9,10]. Additionally, it was suggested that mesenchymal stem cells can be combined with bone morphogenetic protein-2 to enhance bone formation in early healing period [10]. Insulin-like growth factors (IGFs) control various functions, including differentiation and mitogenesis, and IGFs are reported to regulate the bone growth and maintenance [11].…”

Section: Introductionmentioning

confidence: 99%

The Role of Insulin-Like Growth Factor-2 on the Cellular Viability and Differentiation to the Osteogenic Lineage and Mineralization of Stem Cells Cultured on Deproteinized Bovine Bone Mineral

Lee

Min

Park³

et al. 2020

Applied Sciences

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Insulin-like growth factors (IGFs) plays various roles, including differentiation and mitogenesis, and IGFs are reported to regulate the bone growth and maintenance. This study was performed to analyze the enhancing effects of IGF-2 on osteogenic differentiation and the mineralization of stem cells cultured on deproteinized bovine bone mineral. Stem cell loaded bone graft material was cultured in the presence of the IGF-2 at final concentrations of 10 and 100 ng/mL and the morphology of the cells was observed on Days 1, 3, and 7. The commercially available, two-color assay based on plasma membrane integrity and esterase activity was also used for qualitative analyses on Days 1, 3, and 7. The level of alkaline phosphatase activity and anthraquinone dye assay were used to evaluate osteogenic differentiation on Days 7 and 14. Real-time polymerase chain reaction was applied in order to identify the mRNA expression of BGLAP, Runx2, and β-catenin. The stem cells were well-attached with fibroblast morphology and most of the stem cells produced a high intensity of green fluorescence, indicating that there were live cells on Day 1. The relative cellular viability assay values for IGF-2 groups at 0, 10, and 100 ng/mL on Day 1 were 0.419 ± 0.015, 0.427 ± 0.013, and 0.500 ± 0.030, respectively (p < 0.05). The absorbance values at 405 nm for alkaline phosphatase activity on Day 7 for IGF-2 at 0, 10, and 100 ng/mL were 2.112 ± 0.152, 1.897 ± 0.144, and 2.067 ± 0.128, respectively (p > 0.05). The mineralization assay results at Day 7 showed significantly higher values for IGF-2 groups at 10 and 100 ng/mL concentration when compared to the control (p < 0.05). The application of IGF-2 groups of 10 and 100 ng/mL produced a significant increase of BGLAP. Conclusively, this study indicates that the use of IGF-2 on stem cell loaded bone graft increased cellular viability, Alizarin red staining, and BGLAP expression of stem cells. This report suggests the combined approach of stem cells and IGF-2 with scaffold may have synergistic effects on osteogenesis.

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Combinatorial effect of stem cells derived from mandible and recombinant human bone morphogenetic protein-2

Cited by 9 publications

References 25 publications

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

The Use of Electrospun Organic and Carbon Nanofibers in Bone Regeneration

The Role of Insulin-Like Growth Factor-2 on the Cellular Viability and Differentiation to the Osteogenic Lineage and Mineralization of Stem Cells Cultured on Deproteinized Bovine Bone Mineral

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